High performance liquid chromatography with a specific dual detection system for the determination of acetic anhydride: An effective approach to analytical confirmation

1992 ◽  
Vol 15 (7) ◽  
pp. 479-481
Author(s):  
Weh S. Wu ◽  
Virindar S. Gaind
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Acetic Anhydride ◽  
High Performance ◽  
Detection System ◽  
Dual Detection

Review: Determination of Vitamin D in Dairy Products by High Performance Liquid Chromatography

2005 ◽  
Vol 11 (6) ◽  
pp. 451-462 ◽  
Author(s):  
S. Perales ◽  
A. Alegría ◽  
R. Barberá ◽  
R. Farré
Keyword(s):  
Vitamin D ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dairy Products ◽  
High Performance ◽  
Detection System ◽  
Chromatographic Determination ◽  
Sample Treatment ◽  
Cold Saponification

This work reviews the methods used for the determination of vitamin D in some dairy products (milk and infant formulas) by high performance liquid chromatography (HPLC). The low vitamin D contents and the presence of interfering compounds require sample treatment and purification of the extract. The advantages and drawbacks of hot and cold saponification, direct extraction and different types of extract purification are also discussed, taking into account the lack of vitamin D stability by heating, exposure to light and oxidation. With respect to chromatographic determination, the mode (normal or reverse phase), type of column (microcolumn or conventional) and detection system (UV, electrochemical and mass spectrometry) are overviewed, considering the vitamers to be determined.

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