Structural features of forming and developing blood capillaries of the enamel organ of rat molar tooth germs observed by light and electron microscopy

2005 ◽  
Vol 265 (3) ◽  
pp. 335-342 ◽  
Author(s):  
E. Manzke ◽  
E. Katchburian ◽  
F.P. Faria ◽  
E. Freymüller
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Structural Features ◽  
Molar Tooth ◽  
Enamel Organ ◽  
Blood Capillaries ◽  
Tooth Germs ◽  
Rat Molar

An autoradiographic study of calcium movement in the enamel organ of rat molar tooth germs

1990 ◽  
Vol 35 (11) ◽  
pp. 899-906 ◽  
Author(s):  
M. Hanawa ◽  
Y. Takano ◽  
M. Wakita
Keyword(s):  
Autoradiographic Study ◽  
Molar Tooth ◽  
Enamel Organ ◽  
Tooth Germs ◽  
Rat Molar

Correlated 3D Light and Electron Microscopy of Large, Complex Structures: Analysis of Transverse Tubules in Heart Failure

1998 ◽  
Vol 4 (S2) ◽  
pp. 440-441
Author(s):  
Maryann E. Martone ◽  
Andrea Thor ◽  
Stephen J. Young ◽  
Mark H. Ellisman.
Keyword(s):  
Electron Microscopy ◽  
Electron Tomography ◽  
Light And Electron Microscopy ◽  
Structural Features ◽  
Electron Microscopic Level ◽  
Specimen Preparation ◽  
Large Sample Size ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Light microscopic imaging has experienced a renaissance in the past decade or so, as new techniques for high resolution 3D light microscopy have become readily available. Light microscopic (LM) analysis of cellular details is desirable in many cases because of the flexibility of staining protocols, the ease of specimen preparation and the relatively large sample size that can be obtained compared to electron microscopic (EM) analysis. Despite these advantages, many light microscopic investigations require additional analysis at the electron microscopic level to resolve fine structural features.High voltage electron microscopy allows the use of relatively thick sections compared to conventional EM and provides the basis for excellent new methods to bridge the gap between microanatomical details revealed by LM and EM methods. When combined with electron tomography, investigators can derive accurate 3D data from these thicker specimens. Through the use of correlated light and electron microscopy, 3D reconstructions of large cellular or subcellular structures can be obtained with the confocal microscope,

Changes of lectin staining pattern of the Golgi stack during differentiation of the ameloblast in developing rat molar tooth germs

1993 ◽  
Vol 236 (2) ◽  
pp. 355-365 ◽  
Author(s):  
Saburou Matsuo ◽  
Hiroyuki Ichikawa ◽  
Kojiro Kurisu ◽  
Satoshi Wakisaka ◽  
Ken-Ichi Kiyomiya ◽  
...  
Keyword(s):  
Staining Pattern ◽  
Molar Tooth ◽  
Golgi Stack ◽  
Lectin Staining ◽  
Tooth Germs ◽  
Developing Rat ◽  
Rat Molar

Localization of two distinct acid phosphatases in secretory ameloblasts of rat molar tooth germs

1989 ◽  
Vol 34 (8) ◽  
pp. 599-608 ◽  
Author(s):  
S. Matsuo ◽  
H. Nakahara ◽  
Y. Takano ◽  
H. Ichikawa ◽  
S. Wakisaka ◽  
...  
Keyword(s):  
Molar Tooth ◽  
Acid Phosphatases ◽  
Tooth Germs ◽  
Rat Molar

scholarly journals Altered Hypoxia-Induced and Heat Shock Protein Immunostaining in Secondary Hair Follicles Associated with Changes in Altitude and Temperature in Tibetan Cashmere Goats

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2798
Author(s):  
Yanyu He ◽  
Xiu Liu ◽  
Jie De ◽  
Saihong Kang ◽  
John S. Munday
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Structural Features ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Gansu Province ◽  
Outer Root Sheath ◽  
Average Temperature ◽  
Root Sheath ◽  
Cashmere Goats

This experiment compared secondary hair follicles (SFs) in Tibetan cashmere goats from two different steppes that were at different altitudes and had different temperatures. Twenty-four 2-year-old goats were studied. Twelve goats were from Rikaze in Tibet which is at an altitude of above 5000 m with an average temperature of 0 °C. The other 12 studied goats were from Huan County of Gansu Province which is around 2000 m above sea level with an average temperature of 9.2 °C. The structural features of SFs were assessed using light microscopy and transmission electron microscopy. The presence of HIF-1a, HIF-2a, HIF-3a, HSP27, and HOXC13 proteins was studied using immunohistochemistry and immunofluorescence. Light and electron microscopy revealed that the SFs of the Tibetan cashmere goats that lived in the Rikaze Steppe were in the proanagen stage in May. However, the SFs of the goats from the lower warmer Huan County were in the anagen stage at the same time. Immunohistochemistry revealed intense immunostaining for HIF-1a protein in the inner root sheath (IRS) and hair shaft (HS); immunostaining against HIF-2a in the outer root sheath (ORS) and IRS; HIF-3a protein immunostaining in the ORS; HSP27 immunostaining in the ORS, IRS, and HS; and HOXC13 immunostaining in the ORS and HS. HIF-1a protein expression in the IRS and HS was higher than the expression in the ORS (p < 0.05) while the expression of HIF-2a protein was higher in the ORS and IRS than the HS (p < 0.05). The expression of HIF-3a protein was higher in the ORS than in the IRS (p < 0.05). Expression of HOXC13 protein was higher in the ORS than in the IRS and HS (p < 0.05). Immunostaining of HIF-1a, HIF-2a, and HSP27 protein was significantly higher in SFs from cashmere goats from Rikaze than in goats from Huan (p < 0.05). In contrast, HOX13 protein immunostaining was significantly higher in cashmere goats from Huan than from Rikaze (p < 0.05). Significant differences were observed in the SFs of cashmere goats from two locations that differ in altitude and temperature. This suggests the differences in the secondary hair follicles could be due to the hypoxia and lower temperatures experienced by the goats in Rikaze. These results are useful in understanding how altitude and temperature influence SF development. Hair produced by the SFs are used for down fiber. Therefore, understanding of the factors that influence SF development will allow the production and harvest of these valuable fibers to be maximized.

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