Chemical binding of pyrrolidinyl peptide nucleic acid ( acpcPNA‐T9 ) probe with AuNPs toward label‐free monitoring of miRNA ‐21: A novel biosensing platform for biomedical analysis and POC diagnostics

2021 ◽  
Author(s):  
Nazanin Fathi ◽  
Arezoo Saadati ◽  
Mohammad Hasanzadeh ◽  
Mohammad Samiei
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Label Free ◽  
Biomedical Analysis ◽  
Chemical Binding

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating

2013 ◽  
Vol 18 (5) ◽  
pp. 057004 ◽  
Author(s):  
Alessandro Candiani ◽  
Alessandro Bertucci ◽  
Sara Giannetti ◽  
Maria Konstantaki ◽  
Alex Manicardi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Optical Fiber ◽  
Fiber Bragg Grating ◽  
Peptide Nucleic Acid ◽  
Dna Biosensor ◽  
Bragg Grating ◽  
Label Free ◽  
Microstructured Optical Fiber ◽  
Free Dna ◽  
Optical Fiber Bragg Grating

scholarly journals Rapid label-free visual detection of KRAS mutations using peptide nucleic acid and unmodified gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (77) ◽  
pp. 48554-48560 ◽  
Author(s):  
Xihong Zhao ◽  
Chii-Wann Lin
Keyword(s):  
Colorectal Cancer ◽  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Visual Detection ◽  
Label Free ◽  
Kras Mutations

Colorectal cancer (CRC) is among the most commonly diagnosed cancers worldwide.

scholarly journals Label-Free Detection of Zeptomol miRNA via Peptide Nucleic Acid Hybridization Using Novel Cyclic Voltammetry Method

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 836
Author(s):  
Shintaro Takase ◽  
Kouta Miyagawa ◽  
Hisafumi Ikeda
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Microelectrode Array ◽  
Pcr Amplification ◽  
Nucleic Acid Hybridization ◽  
Detection Sensitivity ◽  
Cyclic Voltammograms ◽  
Dna Duplexes ◽  
Label Free ◽  
Label Free Detection

To harness the applicability of microribonucleic acid (miRNA) as a cancer biomarker, the detection sensitivity of serum miRNA needs to be improved. This study evaluated the detection sensitivity of miRNA hybridization using cyclic voltammograms (CVs) and microelectrode array chips modified with peptide nucleic acid (PNA) probes and 6-hydroxy-1-hexanethiol. We investigated the PNA probe modification pattern on array chips using fluorescently labeled cDNA. The pattern was not uniformly spread over the working electrode (WE) and had a one-dimensional swirl-like pattern. Accordingly, we established a new ion-channel sensor model wherein the WE is negatively biased through the conductive π–π stacks of the PNA/DNA duplexes. This paper discusses the mechanism underlying the voltage shift in the CV curves based on the electric double-layer capacitance. Additionally, the novel hybridization evaluation parameter ΔE is introduced. Compared to conventional evaluation using oxidation current changes, ΔE was more sensitive. Using ΔE and a new hybridization system for ultrasmall amounts of aqueous solutions (as low as 35 pL), 140 zeptomol label-free miRNA were detected without polymerase chain reaction (PCR) amplification at an adequate sensitivity. Herein, the differences in the target molar amount and molar concentration are elucidated from the viewpoint of hybridization sensitivity.

scholarly journals Hybridization-Modulated Ion Fluxes through Peptide-Nucleic-Acid- Functionalized Gold Nanotubes. A New Approach to Quantitative Label-Free DNA Analysis

Nano Letters ◽  
2007 ◽  
Vol 7 (6) ◽  
pp. 1609-1612 ◽  
Author(s):  
Gyula Jágerszki ◽  
Róbert E. Gyurcsányi ◽  
Lajos Höfler ◽  
Ernö Pretsch
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Dna Analysis ◽  
Ion Fluxes ◽  
Label Free ◽  
New Approach ◽  
Free Dna

Label-free peptide nucleic acid biosensor for visual detection of multiple strains of influenza A virus suitable for field applications

2020 ◽  
Vol 1093 ◽  
pp. 123-130 ◽  
Author(s):  
Naveen Kumar ◽  
Sandeep Bhatia ◽  
Atul Kumar Pateriya ◽  
Richa Sood ◽  
S. Nagarajan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Influenza A Virus ◽  
Peptide Nucleic Acid ◽  
Influenza A ◽  
Visual Detection ◽  
Label Free ◽  
Free Peptide ◽  
Multiple Strains
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