Detection and typing of respiratory adenoviruses in a single-tube multiplex polymerase chain reaction

The present study was conducted to investigate the occurrence of Vibrio spp. from selected rivers in Kuching,Sarawak (Malaysia) using Multiplex Polymerase Chain Reaction (m-PCR). During the 6-month study period,19 samples were collected monthly from 7 rivers, followed by simultaneous detection of three Vibrio spp.,Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus, in a single tube PCR reaction. Three sets ofprimers targeting the thermolabile (tl), outer membrane protein (ompW) and hemolysin/cytolysin genes(vulCulsl) of V. parahaemolyticus, V. cholerae and V. vulnificus, respectively, were used. The results indicatedthat V. parahaemolyticus was the predominant species, occurring approximately 60.9% throughout thesampling period, followed by V. cholerae (23.1%) and V. vulnificus (16.0%). The months of July andDecember were found to be the months where all three Vibrio spp. were found to be at higher frequencies inthe river samples. Results analyzed also indicated that the rivers with the highest prevalence of the three Vibriospp. were Tambak Sejingkat, followed by Sungai Jernang and Sungai Tabuan. We conclude that m-PCR is apowerful and useful tool for the rapid and simultaneous detection of V. parahaemolyticus, V. cholerae and V.vulnificus from the riverine environments without the need for isolation and culturing. Furthermore, thismethod is highly specific, and could be applied in diagnostic laboratories for larger scale epidemiologicalinvestigations of Vibrio spp.

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Single tube multiplex polymerase chain reaction genotype analysis of GSTM1, GSTT1 and GSTP1: relation of genotypes to TP53 tumor status and clinicopathological variables in breast cancer patients

Pharmacogenetics ◽

10.1097/00008571-199810000-00009 ◽

1998 ◽

Vol 8 (5) ◽

pp. 441-447 ◽

Cited By ~ 60

Author(s):

Vessela Nedelcheva Kristensen ◽

Tone Ikdahl Andersen ◽

Bjorn Krikstein ◽

Gry Geitvik ◽

Eva Skovlund ◽

...

Keyword(s):

Breast Cancer ◽

Polymerase Chain Reaction ◽

Cancer Patients ◽

Multiplex Polymerase Chain Reaction ◽

Breast Cancer Patients ◽

Chain Reaction ◽

Single Tube ◽

Genotype Analysis ◽

Polymerase Chain ◽

Clinicopathological Variables

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Evaluation of a Single-Tube Multiplex Polymerase Chain Reaction Screen for Detection of Common alpha-Thalassemia Genotypes in a Clinical Laboratory

American Journal of Clinical Pathology ◽

10.1309/3vk2-ucj1-5gbj-qv8q ◽

2002 ◽

Vol 118 (1) ◽

pp. 18-24 ◽

Cited By ~ 8

Author(s):

Alana K. Bergstrome Jones ◽

Annette Poon

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Clinical Laboratory ◽

Chain Reaction ◽

Single Tube ◽

Alpha Thalassemia ◽

Polymerase Chain

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Molecular Detection of alpha Thalassemia: A Review of Prevalent Techniques

Medeniyet Medical Journal ◽

10.5222/mmj.2021.14603 ◽

2021 ◽

Author(s):

Divashini Vijian ◽

Wan Suriana Wan Ab Rahman ◽

Kannan Thirumulu Ponnuraj ◽

Zefarina Zulkafli ◽

Noor Haslina Mohd Noor

Keyword(s):

Polymerase Chain Reaction ◽

Multiplex Polymerase Chain Reaction ◽

Complete Blood Count ◽

Globin Gene ◽

Molecular Techniques ◽

Chain Reaction ◽

Single Tube ◽

Advantages And Disadvantages ◽

Alpha Thalassemia ◽

Polymerase Chain

Alpha thalassemia (α-thalassemia) is an autosomal recessive disorder due to the reduction or absence of α globin chain production. Laboratory diagnosis of α-thalassemia requires molecular analysis for the confirmatory diagnosis. A screening test, comprising complete blood count, blood smear and hemoglobin quantification by high performance liquid chromatography and capillary electrophoresis, may not possibly detect all the thalassemia diseases. This review focused on the molecular techniques used to detect α-thalassemia, and the advantages and disadvantages of each technique were highlighted. Multiplex gap-polymerase chain reaction, single-tube multiplex polymerase chain reaction, multiplex ligation-dependent probe amplification, and loop-mediated isothermal amplification were used to detect common deletion of α-thalassemia. Furthermore, the reverse dot blot analysis and a single tube multiplex polymerase chain reaction could detect non-deletion mutation of the α-globin gene. Sanger sequencing is widely used to detect non-deletion mutations of α-thalassemia. Recently, next-generation sequencing was introduced in the diagnosis of both deletion and point mutations of α-thalassemia. Despite the advantages and disadvantages of different techniques, the routine method employed in the laboratory should be based on the facility, expertise, available equipment, and economic conditions.

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