scholarly journals Comparison of Luminex xTAG® RVP fast assay and real time RT-PCR for the detection of respiratory viruses in adults with community-acquired pneumonia

2016 ◽  
Vol 88 (7) ◽  
pp. 1173-1179 ◽  
Author(s):  
Vivian Luchsinger ◽  
Yara Prades ◽  
Mauricio Ruiz ◽  
Rolando Pizarro ◽  
Patricio Rossi ◽  
...  
Keyword(s):  
Real Time ◽  
Respiratory Viruses ◽  
Community Acquired Pneumonia ◽  
Rt Pcr

scholarly journals Self-Sampling of Oropharyngeal Swabs Among Healthcare Workers for Molecular Detection of Respiratory Viruses: A Valuable Approach for Epidemiological Studies and Surveillance Programs

2020 ◽  
Vol 8 ◽  
Author(s):  
Cristina Galli ◽  
Laura Pellegrinelli ◽  
Gabriele Del Castillo ◽  
Giovanni Forni ◽  
Cecilia Eugenia Gandolfi ◽  
...  
Keyword(s):  
Real Time ◽  
Healthcare Workers ◽  
Molecular Detection ◽  
Respiratory Viruses ◽  
Epidemiological Studies ◽  
Influenza Viruses ◽  
Rt Pcr ◽  
Surveillance Programs ◽  
Group 2 ◽  
Group 1

This study aimed at assessing the validity of self-collected (self-sampled) oropharyngeal (OP) swabs among healthcare workers compared to those collected by trained sentinel general practitioners (GP-sampled) from individuals with influenza-like illness (ILI), to be implemented in epidemiological studies and/or surveillance programs of viral pathogens involved in community respiratory infections. In our study, OP swabs were collected from adults (>18 years) with ILI during the 2018–2019 influenza season. Two groups of samples were considered: group 1−131 self-sampled OP swabs collected by healthcare workers after being trained on the sampling procedure; group 2−131 GP-sampled OP swabs collected from outpatients by sentinel GPs operating within the Italian Influenza Surveillance Network. To assess swabbing quality, following RNA extraction, each sample was tested for the presence of the human ribonuclease P gene (RNP) by in-house real-time reverse transcriptase–polymerase chain reaction (RT-PCR). Samples with a cycle threshold (Ct) <35 were considered adequate for further virological analysis. Influenza viruses (IVs), respiratory syncytial virus (RSV), and rhinovirus (RV) genomes were detected by in-house real-time RT-PCR. All samples were positive to RNP detection with Ct <35. The mean Ct value was similar in the two groups (group 1 vs. group 2: 25.93 ± 2.22 vs. 25.46 ± 2.40; p = 0.10). IVs, RSV, and RV positivity rates were 26.7 vs. 52.7% (p < 0.01), 7.6 vs. 9.9% (p = 0.52), and 21.4 vs. 19.9% (p = 0.76), respectively. Self-sampled OP swabs resulted as valid as GP-sampled OP swabs for molecular detection of respiratory viruses. Self-swabbing can thus be a worthwhile strategy for sample collection to implement molecular surveillance of respiratory pathogens and carry out epidemiological studies, easily reaching a larger population size.

scholarly journals Detection of Common Respiratory Viruses in Patients with Acute Respiratory Infections Using Multiplex Real-Time RT-PCR

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Niloofar Neisi ◽  
Samaneh Abbasi ◽  
Manoochehr Makvandi ◽  
Shokrollah Salmanzadeh ◽  
Somayeh Biparva ◽  
...  
Keyword(s):  
Real Time ◽  
Respiratory Infections ◽  
Respiratory Viruses ◽  
Acute Respiratory Infections ◽  
Rt Pcr

scholarly journals A multiplex real-time RT-PCR for simultaneous detection of four most common avian respiratory viruses

Virology ◽  
2018 ◽  
Vol 515 ◽  
pp. 29-37 ◽  
Author(s):  
Nacira Laamiri ◽  
Rim Aouini ◽  
Boutheina Marnissi ◽  
Abdeljelil Ghram ◽  
Issam Hmila
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Respiratory Viruses ◽  
Rt Pcr

scholarly journals Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens

2020 ◽  
Vol 21 (7) ◽  
pp. 2574 ◽  
Author(s):  
Cyril Chik-Yan Yip ◽  
Chi-Chun Ho ◽  
Jasper Fuk-Woo Chan ◽  
Kelvin Kai-Wang To ◽  
Helen Shuk-Ying Chan ◽  
...  
Keyword(s):  
Real Time ◽  
Limit Of Detection ◽  
Respiratory Viruses ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Clinical Specimens ◽  
Coronavirus Infection ◽  
Polymerase Chain ◽  
Novel Coronavirus

The pandemic novel coronavirus infection, Coronavirus Disease 2019 (COVID-19), has affected at least 190 countries or territories, with 465,915 confirmed cases and 21,031 deaths. In a containment-based strategy, rapid, sensitive and specific testing is important in epidemiological control and clinical management. Using 96 SARS-CoV-2 and 104 non-SARS-CoV-2 coronavirus genomes and our in-house program, GolayMetaMiner, four specific regions longer than 50 nucleotides in the SARS-CoV-2 genome were identified. Primers were designed to target the longest and previously untargeted nsp2 region and optimized as a probe-free real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. The new COVID-19-nsp2 assay had a limit of detection (LOD) of 1.8 TCID50/mL and did not amplify other human-pathogenic coronaviruses and respiratory viruses. Assay reproducibility in terms of cycle threshold (Cp) values was satisfactory, with the total imprecision (% CV) values well below 5%. Evaluation of the new assay using 59 clinical specimens from 14 confirmed cases showed 100% concordance with our previously developed COVID-19-RdRp/Hel reference assay. A rapid, sensitive, SARS-CoV-2-specific real-time RT-PCR assay, COVID-19-nsp2, was developed.

scholarly journals Comparison of the conventional multiplex RT-PCR, real time RT-PCR and Luminex xTAG®RVP fast assay for the detection of respiratory viruses

2015 ◽  
Vol 88 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Manohar L. Choudhary ◽  
Siddharth P. Anand ◽  
Shamal A. Tikhe ◽  
Atul M. Walimbe ◽  
Varsha A. Potdar ◽  
...  
Keyword(s):  
Real Time ◽  
Respiratory Viruses ◽  
Rt Pcr

Detection of respiratory viruses and Legionella spp. by real-time polymerase chain reaction in patients with community acquired pneumonia

2009 ◽  
Vol 41 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Bram M.W. Diederen ◽  
Menno M. Van Der Eerden ◽  
Fer Vlaspolder ◽  
Wim G. Boersma ◽  
Jan A.J.W. Kluytmans ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Respiratory Viruses ◽  
Community Acquired Pneumonia ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses

2013 ◽  
Vol 57 (4) ◽  
pp. 338-342 ◽  
Author(s):  
Chi Hyun Cho ◽  
Bayarjavkhlan Chulten ◽  
Chang Kyu Lee ◽  
Myung Hyun Nam ◽  
Soo Young Yoon ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Respiratory Viruses ◽  
Rt Pcr

Evaluation of the incidence of identified types of respiratory viruses in a selected population of inhabitants of the kuyavian-pomeranian voivodeship

2018 ◽  
Vol 54 (3) ◽  
pp. 151-158
Author(s):  
Małgorzata Smoguła ◽  
Marta Pawłowska ◽  
Celestyna Mila-Kierzenkowska ◽  
Joanna Malinowska ◽  
Jerzy Kasprzak ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Real Time ◽  
Genetic Material ◽  
Respiratory Viruses ◽  
Influenza B Virus ◽  
Influenza B ◽  
Rt Pcr ◽  
Syncytial Virus ◽  
Tract Infections ◽  
Epidemiological Situation

Introduction: Respiratory tract infections are caused by various factors including respiratory viruses, for example influenza virus and respiratory syncytial virus (RSV). Surveillance of influenza and other respiratory viruses is carried out as part of the SENTINEL and NON SENTINEL programs in Poland.<br>Aim of study: Determination of the frequency of selected types of influenza and RSV viruses using real-time reverse transcription polymerase chain reaction method (real-time RT-PCR) in a selected population of inhabitants of the kuyavian-pomeranian voivodeship in the epidemic season 2017/2018, i.e. from 1 October 2017 to 30 April 2018.<br>Material and methods: 201 throat and nasal swabs were tested using real-time RT-PCR method for the detection of the presence of genetic material RNA of influenza virus and RSV. The study population was divided into 7 groups depending on age.<br>Results. Positive samples accounted for 48.26% of the swabs tested. The genetic material RNA of influenza virus was found in 95 (47.26) samples and RSV in 2 (1,00%) samples. Influenza B virus was most frequently isolated. The most prevalence of infections was observed in people under 25 years of age.<br>Conclusions: Surveillance of influenza in the SENTINEL and NON SENTINEL programs constitute an important role in assessing the virological and epidemiological situation prevailing in a given area. The results cover part of the population, hence the data may be underestimated. It is necessary to continue studies and observe the virological and epidemiological situation in the polish population.

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