scholarly journals Preparation of highly specific monoclonal antibodies against SARS‐CoV‐2 nucleocapsid protein and the preliminary development of antigen detection test strips

2021 ◽  
Author(s):  
Chengzuo Xie ◽  
Haojie Ding ◽  
Jianzu Ding ◽  
Yangji Xue ◽  
Shaohong Lu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Antigen Detection ◽  
Test Strips

scholarly journals Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests

2021 ◽  
pp. 100311
Author(s):  
Yutaro Yamaoka ◽  
Kei Miyakawa ◽  
Sundararaj Stanleyraj Jeremiah ◽  
Rikako Funabashi ◽  
Koji Okudela ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Antigen Detection ◽  
Epitope Identification

scholarly journals Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein

2016 ◽  
Vol 10 (4) ◽  
pp. e0004595 ◽  
Author(s):  
Aiko Fukuma ◽  
Shuetsu Fukushi ◽  
Tomoki Yoshikawa ◽  
Hideki Tani ◽  
Satoshi Taniguchi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Virus Antigen ◽  
Antigen Detection ◽  
Severe Fever

SARS Coronavirus Nucleocapsid Protein Monoclonal Antibodies Developed Using a Prokaryotic Expressed Protein

Hybridoma ◽  
2011 ◽  
Vol 30 (5) ◽  
pp. 481-485 ◽  
Author(s):  
Juan Zhang ◽  
Ding Wang ◽  
Yue Li ◽  
Qing Zhao ◽  
Ailong Huang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Sars Coronavirus

scholarly journals Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19

2021 ◽  
Vol 22 (22) ◽  
pp. 12412
Author(s):  
Ruei-Min Lu ◽  
Shih-Han Ko ◽  
Wan-Yu Chen ◽  
Yu-Ling Chang ◽  
Hsiu-Ting Lin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Limit Of Detection ◽  
Wide Spectrum ◽  
Mitigation Strategies ◽  
Respiratory Pathogens ◽  
Wild Type ◽  
Human Coronavirus ◽  
Spectrum Detection ◽  
Clinical Detection

Mitigation strategies of the coronavirus disease 2019 (COVID-19) pandemic have been greatly hindered by the continuous emergence of SARS-CoV-2 variants. New sensitive, rapid diagnostic tests for the wide-spectrum detection of viral variants are needed. We generated a panel of 41 monoclonal antibodies against the SARS-CoV-2 nucleocapsid protein (NP) by using mice hybridoma techniques. Of these mAbs, nine exhibited high binding activities and were applied in latex-based lateral flow immunoassays (LFIAs). The LFIAs utilizing NP-mAb-7 and -40 had the best sensitivity and lowest limit of detection: 8 pg for purified NP and 625 TCID50/mL for the authentic virus (hCoV-19/Taiwan/4/2020). The specificity tests showed that the NP-mAb-40/7 LFIA strips did not cross-react with five human coronavirus strains or 20 other common respiratory pathogens. Importantly, we found that 10 NP mutants, including alpha (B.1.1.7), beta (B.1.351), gamma (P.1), and delta (B.1.617.2) variants, could be detected by NP-mAb-40/7 LFIA strips. A clinical study (n = 60) of the NP-mAb-40/7 LFIA strips demonstrated a specificity of 100% and sensitivity of 90% in infected individuals with cycle threshold (Ct) values < 29.5. These anti-NP mAbs have strong potential for use in the clinical detection of SARS-CoV-2 infection, whether the virus is wild-type or a variant of concern.

Development of monoclonal antibodies to Rift Valley Fever Virus and their application in antigen detection and indirect immunofluorescence

2018 ◽  
Vol 460 ◽  
pp. 36-44 ◽  
Author(s):  
Claudia Mroz ◽  
Kristina M. Schmidt ◽  
Sven Reiche ◽  
Martin H. Groschup ◽  
Martin Eiden
Keyword(s):  
Monoclonal Antibodies ◽  
Indirect Immunofluorescence ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Antigen Detection ◽  
Fever Virus ◽  
Valley Fever

Production and Characterization of Monoclonal Antibodies That React to the Nucleocapsid Protein of Avian Metapneumovirus Subtype C

2006 ◽  
Vol 50 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Qingzhong Yu ◽  
Carlos N. Estevez ◽  
Darrell R. Kapczynski
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Subtype C ◽  
Avian Metapneumovirus

scholarly journals Development, Characterization, and Application of Monoclonal Antibodies against Severe Acute Respiratory Syndrome Coronavirus Nucleocapsid Protein

2010 ◽  
Vol 17 (12) ◽  
pp. 2033-2036 ◽  
Author(s):  
Dipankar Das ◽  
Sriram Kammila ◽  
Mavanur R. Suresh
Keyword(s):  
Monoclonal Antibodies ◽  
Severe Acute Respiratory Syndrome ◽  
Western Blotting ◽  
Nucleocapsid Protein ◽  
Epitope Mapping ◽  
High Specificity ◽  
Diagnostic Assays ◽  
Hybridoma Technology ◽  
Recombinant Nucleocapsid Protein

ABSTRACT Five monoclonal antibodies (MAbs) against recombinant nucleocapsid protein (NP) of severe acute respiratory syndrome (SARS)-causing coronavirus (CoV) were developed by hybridoma technology. Epitope mapping by Western blotting showed that these anti-SARS-CoV NP MAbs bind to distinct domains of NP. These anti-SARS-CoV NP MAbs, with their high specificity, are potentially ideal candidates for developing early and sensitive diagnostic assays for SARS-CoV.

scholarly journals Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies.

1979 ◽  
Vol 76 (3) ◽  
pp. 1438-1442 ◽  
Author(s):  
M. Herlyn ◽  
Z. Steplewski ◽  
D. Herlyn ◽  
H. Koprowski
Keyword(s):  
Monoclonal Antibodies ◽  
Colorectal Carcinoma ◽  
Specific Antigen ◽  
Antigen Detection
Sign in / Sign up

Export Citation Format

Share Document