Novel culture strategy for human stem cell proliferation and neuronal differentiation

2007 ◽  
Vol 85 (16) ◽  
pp. 3557-3566 ◽  
Author(s):  
Margarida Serra ◽  
Sofia B. Leite ◽  
Catarina Brito ◽  
Júlia Costa ◽  
Manuel J.T. Carrondo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Neuronal Differentiation ◽  
Human Stem Cell ◽  
Stem Cell Proliferation ◽  
Culture Strategy

scholarly journals Zinc Promotes Adipose-Derived Mesenchymal Stem Cell Proliferation and Differentiation towards a Neuronal Fate

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Mi-Young Moon ◽  
Hyun Jung Kim ◽  
Bo Young Choi ◽  
Min Sohn ◽  
Tae Nyoung Chung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Neurite Outgrowth ◽  
Neuronal Differentiation ◽  
Essential Element ◽  
Treated Group ◽  
Multipotent Stem Cells ◽  
Stem Cell Proliferation ◽  
Proliferation And Differentiation

Zinc is an essential element required for cell division, migration, and proliferation. Under zinc-deficient conditions, proliferation and differentiation of neural progenitors are significantly impaired. Adipose-derived mesenchymal stem cells (AD-MSCs) are multipotent stem cells that can differentiate into neurons. The aim of this study was to evaluate the effect of zinc on AD-MSC proliferation and differentiation. We initially examined the effect of zinc on stem cell proliferation at the undifferentiated stage. AD-MSCs showed high proliferation rates on day 6 in 30 μM and 100 μM of ZnCl2. Zinc chelation inhibited AD-MSC proliferation via downregulation of ERK1/2 activity. We then assessed whether zinc was involved in cell migration and neurite outgrowth during differentiation. After three days of neuronal differentiation, TUJ-1-positive cells were observed, implying that AD-MSCs had differentiated into early neuron or neuron-like cells. Neurite outgrowth was increased in the zinc-treated group, while the CaEDTA-treated group showed diminished, shrunken neurites. Furthermore, we showed that zinc promoted neurite outgrowth via the inactivation of RhoA and led to the induction of neuronal gene expression (MAP2 and nestin) in differentiated stem cells. Taken together, zinc promoted AD-MSC proliferation and affected neuronal differentiation, mainly by increasing neurite outgrowth.

scholarly journals CCL2 induces neural stem cell proliferation and neuronal differentiation in Niemann-Pick type C mice

2015 ◽  
Vol 77 (6) ◽  
pp. 693-699 ◽  
Author(s):  
Yu Ri HONG ◽  
Hyun LEE ◽  
Min Hee PARK ◽  
Jong Kil LEE ◽  
Ju Youn LEE ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Neuronal Differentiation ◽  
Neural Stem Cell ◽  
Stem Cell Proliferation ◽  
Neural Stem Cell Proliferation ◽  
Type C ◽  
Niemann Pick

scholarly journals Methotrexate released in vitro from bone cement inhibits human stem cell proliferation in S/G2 phase

2009 ◽  
Vol 34 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Egon Prochazka ◽  
Tomas Soukup ◽  
Milos Hroch ◽  
Leos Fuksa ◽  
Eva Brcakova ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Bone Cement ◽  
Human Stem Cell ◽  
Stem Cell Proliferation ◽  
G2 Phase

Regulators of human stem cell proliferation and engraftment

2002 ◽  
Vol 76 (S1) ◽  
pp. 159-159
Author(s):  
Allen Eaves ◽  
Jody Cashman ◽  
Connie Eaves
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Human Stem Cell ◽  
Stem Cell Proliferation

scholarly journals DICER- and AGO3-dependent generation of retinoic acid–induced DR2 Alu RNAs regulates human stem cell proliferation

2012 ◽  
Vol 19 (11) ◽  
pp. 1168-1175 ◽  
Author(s):  
QiDong Hu ◽  
Bogdan Tanasa ◽  
Michele Trabucchi ◽  
Wenbo Li ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Retinoic Acid ◽  
Human Stem Cell ◽  
Stem Cell Proliferation
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