Zinc Promotes Adipose-Derived Mesenchymal Stem Cell Proliferation and Differentiation towards a Neuronal Fate

Zinc is an essential element required for cell division, migration, and proliferation. Under zinc-deficient conditions, proliferation and differentiation of neural progenitors are significantly impaired. Adipose-derived mesenchymal stem cells (AD-MSCs) are multipotent stem cells that can differentiate into neurons. The aim of this study was to evaluate the effect of zinc on AD-MSC proliferation and differentiation. We initially examined the effect of zinc on stem cell proliferation at the undifferentiated stage. AD-MSCs showed high proliferation rates on day 6 in 30 μM and 100 μM of ZnCl2. Zinc chelation inhibited AD-MSC proliferation via downregulation of ERK1/2 activity. We then assessed whether zinc was involved in cell migration and neurite outgrowth during differentiation. After three days of neuronal differentiation, TUJ-1-positive cells were observed, implying that AD-MSCs had differentiated into early neuron or neuron-like cells. Neurite outgrowth was increased in the zinc-treated group, while the CaEDTA-treated group showed diminished, shrunken neurites. Furthermore, we showed that zinc promoted neurite outgrowth via the inactivation of RhoA and led to the induction of neuronal gene expression (MAP2 and nestin) in differentiated stem cells. Taken together, zinc promoted AD-MSC proliferation and affected neuronal differentiation, mainly by increasing neurite outgrowth.

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TET2 deficiency in mesenchymal stem cells alters their ability to support hematopoietic stem cell proliferation and differentiation

Experimental Hematology ◽

10.1016/j.exphem.2017.06.087 ◽

2017 ◽

Vol 53 ◽

pp. S55-S56

Author(s):

Rong Li ◽

Zhigang Zhao ◽

Zizhen Chen ◽

Wen Xing ◽

Weiping Yuan ◽

...

Keyword(s):

Stem Cells ◽

Cell Proliferation ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Hematopoietic Stem Cell ◽

Hematopoietic Stem ◽

Stem Cell Proliferation ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

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Bone Marrow Mesenchymal Stem Cells Stimulate Cardiac Stem Cell Proliferation and Differentiation

Circulation Research ◽

10.1161/circresaha.110.222703 ◽

2010 ◽

Vol 107 (7) ◽

pp. 913-922 ◽

Cited By ~ 519

Author(s):

Konstantinos E. Hatzistergos ◽

Henry Quevedo ◽

Behzad N. Oskouei ◽

Qinghua Hu ◽

Gary S. Feigenbaum ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Cell Proliferation ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cardiac Stem Cell ◽

Stem Cell Proliferation ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation ◽

Marrow Mesenchymal Stem Cells

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Nanomaterial integration into the scaffolding materials for nerve tissue engineering: a review

Reviews in the Neurosciences ◽

10.1515/revneuro-2020-0008 ◽

2020 ◽

Vol 31 (8) ◽

pp. 843-872

Author(s):

Hamidreza Arzaghi ◽

Bashir Adel ◽

Hossein Jafari ◽

Shaghayegh Askarian-Amiri ◽

Amin Shiralizadeh Dezfuli ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Cell Proliferation ◽

Nervous System ◽

Stem Cell ◽

Complex Network ◽

Neural Regeneration ◽

Stem Cell Proliferation ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

AbstractThe nervous system, which consists of a complex network of millions of neurons, is one of the most highly intricate systems in the body. This complex network is responsible for the physiological and cognitive functions of the human body. Following injuries or degenerative diseases, damage to the nervous system is overwhelming because of its complexity and its limited regeneration capacity. However, neural tissue engineering currently has some capacities for repairing nerve deficits and promoting neural regeneration, with more developments in the future. Nevertheless, controlling the guidance of stem cell proliferation and differentiation is a challenging step towards this goal. Nanomaterials have the potential for the guidance of the stem cells towards the neural lineage which can overcome the pitfalls of the classical methods since they provide a unique microenvironment that facilitates cell–matrix and cell–cell interaction, and they can manipulate the cell signaling mechanisms to control stem cells’ fate. In this article, the suitable cell sources and microenvironment cues for neuronal tissue engineering were examined. Afterward, the nanomaterials that impact stem cell proliferation and differentiation towards neuronal lineage were reviewed.

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Pivotal role of Pten in the balance between proliferation and differentiation of hematopoietic stem cells in zebrafish

Blood ◽

10.1182/blood-2013-05-501544 ◽

2014 ◽

Vol 123 (2) ◽

pp. 184-190 ◽

Cited By ~ 27

Author(s):

Suma Choorapoikayil ◽

Rianne Kers ◽

Philippe Herbomel ◽

Karima Kissa ◽

Jeroen den Hertog

Keyword(s):

Stem Cells ◽

Cell Proliferation ◽

Stem Cell ◽

Zebrafish Embryos ◽

Hematopoietic Stem ◽

Stem Cell Proliferation ◽

Key Points ◽

Proliferation And Differentiation ◽

Excellent Tool

Key Points Loss of the tumor suppressor, PTEN, results in enhanced blood stem cell proliferation and arrested differentiation, hallmarks of leukemia. Pten mutant zebrafish embryos display defective hematopoiesis and constitute an excellent tool to assess drug treatment.

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