Purification and characterization of cloned alkaline protease gene ofGeobacillus stearothermophilus

2014 ◽  
Vol 55 (2) ◽  
pp. 160-171 ◽  
Author(s):  
Irfana Iqbal ◽  
Muhammad Nauman Aftab ◽  
Mohammed Afzal ◽  
Asad Ur-Rehman ◽  
Saima Aftab ◽  
...  
2020 ◽  
Vol 8 (2) ◽  
pp. 154-160
Author(s):  
N.G. Ramesh Babu ◽  
Anupa Mary Aji

  Alkaline protease enzymes are enzymes which can catalyze the process of proteolysis between the pH ranges from 8 to 12.  Extracellular alkaline proteases are used as additives in detergent powders. In the present study, source of the organism was from a detergent contaminated area. The study has been carried out in Aeromonas hydrophila AH10 strain that produces protease enzyme with an alkaline pH optimum. The organism was a gram-negative rod with a protease enzyme activity of 0.385 ml/min. purification of the protease enzyme from the bacteria was carried out. This protease is suitable for use in alkaline detergent powders as well as in silver recovery process. The Aeromonas hydrophila strain AH10 gene encoding this high-alkaline protease was cloned and characterized.   Int. J. Appl. Sci. Biotechnol. Vol 8(2): 154-160


1991 ◽  
Vol 55 (11) ◽  
pp. 2807-2811 ◽  
Author(s):  
Kohji Murakami ◽  
Yutaka Ishida ◽  
Atsushi Masaki ◽  
Hiroki Tatsumi ◽  
Seiji Murakami ◽  
...  

1991 ◽  
Vol 55 (11) ◽  
pp. 2807-2811 ◽  
Author(s):  
Kohji MURAKAMI ◽  
Yutaka ISHIDA ◽  
Atsushi MASAKI ◽  
Hiroki TATSUMI ◽  
Seiji MURAKAMI ◽  
...  

1994 ◽  
Vol 77 (5) ◽  
pp. 554-556 ◽  
Author(s):  
Kazuo Saeki ◽  
Junko Iwata ◽  
Yasuo Watanabe ◽  
Youichi Tamai

2005 ◽  
Vol 1075 (1-2) ◽  
pp. 103-108 ◽  
Author(s):  
Anshu Gupta ◽  
Ipsita Roy ◽  
R.K. Patel ◽  
S.P. Singh ◽  
S.K. Khare ◽  
...  

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