Expression, purification, and molecular characterization of a full-length thermostable alkaline protease gene from Bacillus subtilis DMA-09

Biologia ◽  
2020 ◽  
Author(s):  
Dilara Abbas Bukhari ◽  
Amina Barkat ◽  
Abdul Rehman
Keyword(s):  
Bacillus Subtilis ◽  
Molecular Characterization ◽  
Alkaline Protease ◽  
Full Length ◽  
Protease Gene ◽  
Alkaline Protease Gene

scholarly journals Involvement of Stringent Factor RelA in Expression of the Alkaline Protease Gene aprE in Bacillus subtilis

2001 ◽  
Vol 183 (15) ◽  
pp. 4648-4651 ◽  
Author(s):  
Michihiro Hata ◽  
Mitsuo Ogura ◽  
Teruo Tanaka
Keyword(s):  
Bacillus Subtilis ◽  
Alkaline Protease ◽  
Double Mutant ◽  
Regulatory System ◽  
Protease Gene ◽  
Wild Type ◽  
Double Mutation ◽  
Two Component ◽  
In The Wild ◽  
Alkaline Protease Gene

ABSTRACT Expression of Bacillus subtilis aprE, encoding an extracellular alkaline protease, is positively regulated by phosphorylated DegU, the regulator of a two-component regulatory system, DegS-DegU. We found that the expression of anaprE′-′lacZ fusion was greatly reduced in a disruption mutant with a mutation of relA, which encodes the stringent factor RelA. The level of DegU in the relA mutant was similar to that in the wild-type cell. A relA degU double mutation did not result in a further decrease of theaprE′-′lacZ level found in a degU single mutant. The expression of the aprE′-′lacZ fusion in therelA mutant was stimulated by multicopy degR or the degU32(Hy) and degS200(Hy) mutations that cause the stabilization of phosphorylated DegU. Furthermore, the expression of sacB′-′lacZ, which is also dependent on phosphorylated DegU, was stimulated by the relA mutation, and this stimulation was not seen in the relA degU double mutant. These results show that RelA (or its product guanosine-3′,5′-bisdiphosphate [pp Gpp]) does not affect the phosphorylation of DegU and suggest that it participates in the expression of aprE and sacB through the regulation of DegU-dependent transcription.

scholarly journals Molecular Characterization of Alkaline Protease Gene Isolated from Aeromonas hydrophila strain AH10

2020 ◽  
Vol 8 (2) ◽  
pp. 154-160
Author(s):  
N.G. Ramesh Babu ◽  
Anupa Mary Aji
Keyword(s):  
Aeromonas Hydrophila ◽  
Alkaline Protease ◽  
Recovery Process ◽  
Protease Gene ◽  
Gene Encoding ◽  
Silver Recovery ◽  
Ph Optimum ◽  
Protease Enzyme ◽  
Alkaline Protease Gene

  Alkaline protease enzymes are enzymes which can catalyze the process of proteolysis between the pH ranges from 8 to 12.  Extracellular alkaline proteases are used as additives in detergent powders. In the present study, source of the organism was from a detergent contaminated area. The study has been carried out in Aeromonas hydrophila AH10 strain that produces protease enzyme with an alkaline pH optimum. The organism was a gram-negative rod with a protease enzyme activity of 0.385 ml/min. purification of the protease enzyme from the bacteria was carried out. This protease is suitable for use in alkaline detergent powders as well as in silver recovery process. The Aeromonas hydrophila strain AH10 gene encoding this high-alkaline protease was cloned and characterized.   Int. J. Appl. Sci. Biotechnol. Vol 8(2): 154-160

Isolation and Characterization of the Alkaline Protease Gene ofAspergillus oryzae

1991 ◽  
Vol 55 (11) ◽  
pp. 2807-2811 ◽  
Author(s):  
Kohji Murakami ◽  
Yutaka Ishida ◽  
Atsushi Masaki ◽  
Hiroki Tatsumi ◽  
Seiji Murakami ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Protease Gene ◽  
Isolation And Characterization ◽  
Alkaline Protease Gene

scholarly journals Isolation and Characterization of the Alkaline Protease Gene of Aspergillus oryzae.

1991 ◽  
Vol 55 (11) ◽  
pp. 2807-2811 ◽  
Author(s):  
Kohji MURAKAMI ◽  
Yutaka ISHIDA ◽  
Atsushi MASAKI ◽  
Hiroki TATSUMI ◽  
Seiji MURAKAMI ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Alkaline Protease ◽  
Protease Gene ◽  
Isolation And Characterization ◽  
Alkaline Protease Gene

Purification and characterization of cloned alkaline protease gene ofGeobacillus stearothermophilus

2014 ◽  
Vol 55 (2) ◽  
pp. 160-171 ◽  
Author(s):  
Irfana Iqbal ◽  
Muhammad Nauman Aftab ◽  
Mohammed Afzal ◽  
Asad Ur-Rehman ◽  
Saima Aftab ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Protease Gene ◽  
Purification And Characterization ◽  
Alkaline Protease Gene

Molecular characterization of rhesus monkey Mhc-DR region: full length cDRA/cDRB analysis

2003 ◽  
Vol 64 (10) ◽  
pp. S164
Author(s):  
Nanine de Groot ◽  
Gaby G.M. Doxiadis ◽  
Natasja G. de Groot ◽  
Nel Otting ◽  
Corrine M.C. Heijmans ◽  
...  
Keyword(s):  
Rhesus Monkey ◽  
Molecular Characterization ◽  
Full Length
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