S. P. Colowick and N. O. Kaplan (Editors-In-Chief), Methods in Enzymology Volume 121: Immunochemical Techniques, Part I, Hybridoma Technology and Monoclonal Antibodies (Editors: J. J. LANGONE, H. VAN VUNAKIS). XIX + 947 S., 131 Tab., 215 Abb. Orlando-San Diego-New York-Austin-London-Montal-Sydney-Tokyo-Toronto 1986. $ 85.00. ISBN: 0-12-182021-1

1987 ◽  
Vol 27 (7) ◽  
pp. 368-368
Author(s):  
L. Wollweber
Keyword(s):  
New York ◽  
Monoclonal Antibodies ◽  
San Diego ◽  
Hybridoma Technology ◽  
Immunochemical Techniques

PERSPECTIVES OF APPLICATION OF ANTIBODIES AGAINST ENDOGLIN (CD105) FOR VISUALIZATION AND ANTI-ANGIOGENE THERAPY OF TUMORS

2018 ◽  
Vol 64 (4) ◽  
pp. 504-507
Author(s):  
Vladimir Klimovich ◽  
Natalya Vartanyan ◽  
Anastasiya Stolbovaya ◽  
Lidiya Terekhina ◽  
Olga Shashkova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Vascular Endothelium ◽  
Immune Complexes ◽  
Targeted Delivery ◽  
Biological Fluids ◽  
Therapeutic Drugs ◽  
Cultured Endothelial Cells ◽  
Hybridoma Technology

During last years monoclonal antibodies (MAB) directed against vascular endothelium markers demonstrated their efficiency for visualization and targeted delivery of therapeutic drugs to tumors. Endoglin (CD105) which serves as a key element that determines endothelial cells quiescence or activation is one of such markers. Endoglin is highly expressed on the vascular endothelium of growing tumors. A first panel of MAB against endoglin in our country was produced at the hybridoma technology laboratory of RRC RST named after A.M. Granov. On the basis of these MAB ELISA was created allowing detection of endoglin in human plasma and other biological fluids. Several MAB had been shown to bind endoglin on the membrane of the cultured endothelial cells and to persist there for several hours. During the first 30 min after binding some of the immune complexes “endoglin-MAB” were internalized into the cytoplasm and were found included in the endosomes. In future these MAB can be used to create the reagents for the addressed delivery of isotope tags both on the membrane and into the cytoplasm of endothelial cells.

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