Low-intensity pulsed ultrasound stimulation enhances TIMP-1 in nucleus pulposus cells and MCP-1 in macrophages in the rat

2008 ◽  
Vol 26 (6) ◽  
pp. 865-871 ◽  
Author(s):  
Hiroko Omi ◽  
Joji Mochida ◽  
Toru Iwashina ◽  
Raijiro Katsuno ◽  
Akihiko Hiyama ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Pulsed Ultrasound ◽  
Nucleus Pulposus Cells ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

scholarly journals Synergistic effect of low-intensity pulsed ultrasound on growth factor stimulation of nucleus pulposus cells

2007 ◽  
Vol 25 (12) ◽  
pp. 1574-1581 ◽  
Author(s):  
Akihiko Hiyama ◽  
Joji Mochida ◽  
Toru Iwashina ◽  
Hiroko Omi ◽  
Takuya Watanabe ◽  
...  
Keyword(s):  
Growth Factor ◽  
Synergistic Effect ◽  
Nucleus Pulposus ◽  
Pulsed Ultrasound ◽  
Nucleus Pulposus Cells ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Stimulation Of

scholarly journals Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuya Shimizu ◽  
Naomasa Fujita ◽  
Kiyomi Tsuji-Tamura ◽  
Yoshimasa Kitagawa ◽  
Toshiaki Fujisawa ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Transcriptional Control ◽  
Target Genes ◽  
Pulsed Ultrasound ◽  
Activated T Cells ◽  
In Vivo Models ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.

Low-intensity pulsed ultrasound stimulation for mandibular condyle osteoarthritis lesions in rats

Oral Diseases ◽  
2017 ◽  
Vol 24 (4) ◽  
pp. 600-610 ◽  
Author(s):  
A Kanaguchi Arita ◽  
I Yonemitsu ◽  
Y Ikeda ◽  
M Miyazaki ◽  
T Ono
Keyword(s):  
Mandibular Condyle ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation
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