scholarly journals Distribution of slow-cycling cells in epiphyseal cartilage and requirement of β-catenin signaling for their maintenance in growth plate

2014 ◽  
Vol 32 (5) ◽  
pp. 661-668 ◽  
Author(s):  
Maria Elena Candela ◽  
Leslie Cantley ◽  
Rika Yasuaha ◽  
Masahiro Iwamoto ◽  
Maurizio Pacifici ◽  
...  
Keyword(s):  
Growth Plate ◽  
Epiphyseal Cartilage ◽  
Slow Cycling

PTH/PTHrP receptor mRNA is down-regulated in epiphyseal cartilage growth plate of uraemic rats

1996 ◽  
Vol 11 (10) ◽  
pp. 2008-2016 ◽  
Author(s):  
P. Urena ◽  
A. Ferreira ◽  
C. Morieux ◽  
T. Drueke ◽  
M. Christine de Vernejoul
Keyword(s):  
Growth Plate ◽  
Epiphyseal Cartilage ◽  
Cartilage Growth ◽  
Receptor Mrna ◽  
Pthrp Receptor ◽  
Cartilage Growth Plate

scholarly journals Chondrocytes in the resting zone of the growth plate are maintained in a Wnt-inhibitory environment

2020 ◽  
Author(s):  
Shawn A. Hallett ◽  
Wanida Ono ◽  
Yuki Matsushita ◽  
Naoko Sakagami ◽  
Koji Mizuhashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Plate ◽  
Cell Cycle Progression ◽  
Postnatal Growth ◽  
Rna Seq ◽  
Cycle Progression ◽  
Parathyroid Hormone Related Protein ◽  
Significant Enrichment ◽  
Slow Cycling ◽  
Canonical Wnt

AbstractChondrocytes in the resting zone of the postnatal growth plate are characterized by slow cell cycle progression, and encompass a population of parathyroid hormone-related protein (PTHrP)-expressing skeletal stem cells that contribute to the formation of columnar chondrocytes. However, how these chondrocytes are maintained in the resting zone remains undefined. We undertook a genetic pulse-chase approach to isolate slow cycling, label-retaining chondrocytes (LRCs) from the growth plate using a chondrocyte-specific doxycycline-controllable Tet-Off system regulating expression of histone 2B-linked GFP. Comparative RNA-seq analysis identified significant enrichment of inhibitors and activators for Wnt/β-catenin signaling in LRCs and non-LRCs, respectively. Activation of Wnt/β-catenin signaling in PTHrP+ resting chondrocytes using Pthrp-creER and Apc-floxed allele impaired their ability to form columnar chondrocytes. Therefore, slow-cycling chondrocytes are maintained in a canonical Wnt-inhibitory environment within the resting zone, unraveling a novel mechanism regulating maintenance and differentiation of PTHrP+ skeletal stem cells of the postnatal growth plate.

scholarly journals Analisis Pertumbuhan Kartilago Epifisialis Os Tibia Fetus Mencit (Mus musculus L.) Swiss Webster Setelah Induksi Ochratoxin A Selama Periode Organogenesis

2018 ◽  
Vol 4 (1) ◽  
pp. 25-31
Author(s):  
Arum Setiawan ◽  
Mammed Sagi ◽  
Widya Asmara ◽  
Istriyati Istriyati
Keyword(s):  
Key Words ◽  
Ochratoxin A ◽  
Growth Plate ◽  
Mus Musculus ◽  
Epiphyseal Cartilage ◽  
Maturation Zone ◽  
Proliferative Zone ◽  
Key Words Ochratoxin A ◽  
Pregnant Mice ◽  
Tibial Growth Plate

The aims of this study were determined the effects of Ochratoxin A (OTA) on growth of fetus tibia epiphyseal cartilage during organogenesis period. Twenty four pregnant mice were divided randomly into 4 groups of 6. Ochratoxin A was dissolved in sodium bicarbonateand administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining were used as control. The fetal tibia was taken after the 18 th day of pregnancy. The growth of tibia epiphyseal cartilages were observed histologically using Erlich’s Haematoxylin-Eosin Stain. The result of this study indicated that OTA caused decreased thickness of the rest zone, proliferative zone, maturation zone and calsification zone of the fetus tibial growth plate significantly. Key words: Ochratoxin A, tibia, cartilage, and thickness.

Patterns of proteoglycan degradation by a neutral protease from human growth-plate epiphyseal cartilage.

1982 ◽  
Vol 64 (9) ◽  
pp. 1350-1354 ◽  
Author(s):  
M G Ehrlich ◽  
A L Armstrong ◽  
R G Neuman ◽  
M W Davis ◽  
H J Mankin
Keyword(s):  
Growth Plate ◽  
Human Growth ◽  
Epiphyseal Cartilage ◽  
Neutral Protease ◽  
Proteoglycan Degradation

scholarly journals Chondrocytes in the resting zone of the growth plate are maintained in a Wnt-inhibitory environment

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Shawn A Hallett ◽  
Yuki Matsushita ◽  
Wanida Ono ◽  
Naoko Sakagami ◽  
Koji Mizuhashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Plate ◽  
Cell Cycle Progression ◽  
Postnatal Growth ◽  
Related Protein ◽  
Rna Seq ◽  
Cycle Progression ◽  
Parathyroid Hormone Related Protein ◽  
Significant Enrichment ◽  
Slow Cycling

Chondrocytes in the resting zone of the postnatal growth plate are characterized by slow cell cycle progression, and encompass a population of parathyroid hormone-related protein (PTHrP)-expressing skeletal stem cells that contribute to the formation of columnar chondrocytes. However, how these chondrocytes are maintained in the resting zone remains undefined. We undertook a genetic pulse-chase approach to isolate slow cycling, label-retaining chondrocytes (LRCs) using a chondrocyte-specific doxycycline-controllable Tet-Off system regulating expression of histone 2B-linked GFP. Comparative RNA-seq analysis identified significant enrichment of inhibitors and activators for Wnt signaling in LRCs and non-LRCs, respectively. Activation of Wnt/β-catenin signaling in PTHrP+ resting chondrocytes using Pthlh-creER and Apc-floxed allele impaired their ability to form columnar chondrocytes. Therefore, slow-cycling chondrocytes are maintained in a Wnt-inhibitory environment within the resting zone, unraveling a novel mechanism regulating maintenance and differentiation of PTHrP+ skeletal stem cells of the postnatal growth plate.

scholarly journals IRON DEPOSITION IN THE GROWTH PLATE OF LONG BONES OF THE OFFSPRING WHEN GIVEN DURING PREGNANCY IN RAT MODEL

2021 ◽  
Vol 71 (4) ◽  
pp. 1223-26
Author(s):  
Faiza Umbreen ◽  
Khadija Qamar ◽  
Sadia Shaukat ◽  
Maimoona Khan ◽  
Sana Malik ◽  
...  
Keyword(s):  
Growth Plate ◽  
Rat Model ◽  
Iron Supplementation ◽  
Epiphyseal Cartilage ◽  
Iron Deposition ◽  
Epiphyseal Growth Plate ◽  
Adult Rats ◽  
Pregnant Rats ◽  
Male Adult ◽  
Pregnancy And Lactation

Objective: To evaluate histologically the deposition of iron in the epiphyseal cartilage of offspring’s of dams given iron supplementation during pregnancy and lactation in rat model. Study Design: Laboratory based experimental study. Place and Duration of Study: Department of Anatomy, Army Medical College, Rawalpindi and National Institute of Health (NIH) Islamabad, from Mar to Nov 2016. Methodology: In this study, 16 female and 4 male adult rats were chosen for breading. After confirmation of pregnancy, pregnant rats were separated in two groups. One group was given oral iron supplementation for four weeks till delivery and half of the pups fed by mothers who were given iron during lactation. The other group was kept on normal lab diet. Deposition of iron in the epiphyseal cartilage of newborn rats after four weeks was evaluated histologically in pups. Results: Iron deposition was maximum in group C i.e. 1.30 ± 0.48; in group B it was 0.20 ± 0.44. Statistically significant iron deposition (p<0.001) was observed in the growth plate of off springs when mothers were given iron supplements during pregnancy and lactation. Conclusion: Present study proves that injudicious iron supplementation through pregnancy results in deposition of iron in epiphyseal growth plate of the fetus and it can have damaging effects on bones of fetus.

scholarly journals Ultrastructural localization of collagen types II, IX, and XI in the growth plate of human rib and fetal bovine epiphyseal cartilage: type XI collagen is restricted to thin fibrils.

1995 ◽  
Vol 43 (10) ◽  
pp. 967-979 ◽  
Author(s):  
D R Keene ◽  
J T Oxford ◽  
N P Morris
Keyword(s):  
Growth Plate ◽  
Ultrastructural Localization ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Collagen Types ◽  
Amino Terminal ◽  
Proliferative Zone ◽  
Human Newborn ◽  
Fetal Bovine ◽  
Type Xi Collagen

The collagen fibrils of hyaline cartilage vary in diameter depending on developmental stage and location within the tissue. In general, growth plates and fetal epiphyseal cartilages contain fibrils with diameters of less than approximately 25 nm, whereas the permanent cartilage of adult tissues contains fibrils of approximately 30-200 nm. The interstitial collagen fibrils of fetal cartilage are complex, having at least three collagen types as integral components. Type XI, a member of the fibrillar collagen class, has been proposed to limit fibril diameter. To test this proposition we sought to determine if Type XI collagen was preferentially associated with fibrils of smaller diameter. We focused our study on human juvenile rib growth plate, which has thin fibrils in the hypertrophic zone, thick fibrils in the resting zone or permanent cartilage, and a mixture of thin and thick fibrils in the proliferative zone. Tissues were examined by immunoelectron microscopy with antipeptide antibodies to the carboxyl telopeptide and to the amino terminal non-triple-helical domains of alpha 1 (XI). These studies showed that (a) both epitopes of Type XI collagen were readily accessible to antibodies at the fibrillar surface, (b) Type XI collagen was associated predominantly with fibrils < 25 nm in diameter, (c) Type XI collagen was not found in thick fibrils even after disruption with chaotropic agents, and (d) collagen Types II and IX were associated with fibrils of all sizes. These studies were extended to human newborn epiphyseal cartilage and to fetal calf cartilage, with the same result.

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