Sensitive Radioimmunoassay and Enzyme-Linked Immunosorbent Assay for the Simultaneous Determination of Chloroquine and Its Metabolites in Biological Fluids

1990 ◽  
Vol 79 (1) ◽  
pp. 23-27 ◽  
Author(s):  
C. Escande ◽  
P. Chevalier ◽  
F. Verdier ◽  
R. Bourdon
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Biological Fluids ◽  
Enzyme Linked Immunosorbent Assay

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

2012 ◽  
Vol 6 (3) ◽  
pp. 767-774 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Greta Nölke ◽  
Jing Zhang ◽  
Lanlan Niu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Food Matrices

scholarly journals Simultaneous Determination of Florfenicol and Its Metabolite Florfenicol Amine in Swine Muscle Tissue by a Heterologous Enzyme-Linked Immunosorbent Assay

2009 ◽  
Vol 92 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Pengjie Luo ◽  
Haiyang Jiang ◽  
Zhanhui Wang ◽  
Caimao Feng ◽  
Fangyang He ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Muscle Tissue ◽  
Immunosorbent Assay ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A sensitive and heterologous enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of florfenicol (FF) and its metabolite florfenicol amine (FFA) in swine muscle tissue was developed. FFA was conjugated to bovine serum albumin by a formaldehyde coupling method as an immunogen to immunize rabbits. FFA, thiamphenicol glycinate, and modified FF were conjugated to ovalbumin as coating antigens. The effect of different types of hapten heterology on the sensitivity and specificity of the ELISA was evaluated. Using FF glutaric anhydride ester as a coating hapten and antibody raised against modified FFA, an ELISA was developed that showed an IC50 value of 0.48 ng/mL. The antibody showed a cross-reactivity of 100 with FFA, 97 with FF, 6 with thiamphenicol, and a negligible value with chloramphenicol. From fortified swine muscle samples at levels of 4320 ng/g, the average recoveries of FF and FFA ranged from 58.2 to 96.8, with coefficients of variation less than 14. Analysis of incurred samples by the ELISA gave similar results to those by a previously developed liquid chromatographic method. The ELISA could be used as a rapid method for the simultaneous determination of FF and FFA in swine muscle tissue.

Simultaneous Determination of 13 Fluoroquinolone and 22 Sulfonamide Residues in Milk by a Dual-Colorimetric Enzyme-Linked Immunosorbent Assay

2013 ◽  
Vol 85 (4) ◽  
pp. 1995-1999 ◽  
Author(s):  
Wenxiao Jiang ◽  
Zhanhui Wang ◽  
Ross C. Beier ◽  
Haiyang Jiang ◽  
Yongning Wu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sulfonamide Residues

Simultaneous Determination of (Fluoro)quinolone Antibiotics in Kidney, Marine Products, Eggs, and Muscle by Enzyme-Linked Immunosorbent Assay (ELISA)

2006 ◽  
Vol 54 (8) ◽  
pp. 2822-2827 ◽  
Author(s):  
Anne-Catherine Huet ◽  
Caroline Charlier ◽  
Sheryl A. Tittlemier ◽  
Gurmit Singh ◽  
Samuel Benrejeb ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Quinolone Antibiotics ◽  
Marine Products

A monoclonal antibody-based enzyme-linked immunosorbent assay of alpha 1-acid glycoprotein.

1987 ◽  
Vol 33 (12) ◽  
pp. 2275-2277 ◽  
Author(s):  
N H Fraeyman ◽  
E J Van de Velde ◽  
F H De Smet
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Biological Fluids ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rabbit Antibody ◽  
Acid Glycoprotein ◽  
Microtiter Plates ◽  
Sandwich Type ◽  
Human Sera

Abstract A "sandwich"-type enzyme-linked immunosorbent assay for determining concentrations of human alpha 1-acid glycoprotein (AGP) is described. Microtiter plates coated with a polyclonal rabbit antibody to human AGP were subsequently incubated with the antigen, with a specific murine monoclonal antibody, and with goat anti-mouse immunoglobulins conjugated to alkaline phosphatase. To evaluate the method for assay of AGP in human sera, we compared it with single radial immunodiffusion and "rocket" electroimmunoassay. The respective correlations were r = 0.988 (n = 45) and r = 0.973 (n = 47). Repeated assays of a human serum sample with an average AGP concentration of 859 mg/L yielded within-day and between-day CVs of 1.4% (n = 5) and 6.3% (n = 10), respectively. Because of its low detection limit (4.4 micrograms/L), this assay is also suitable for determination of AGP concentrations in other biological fluids, such as dialysates of patients being treated by continuous ambulatory peritoneal dialysis.

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