In vivo determination of fat content in Atlantic salmon (Salmo salar) with a mobile NMR spectrometer

2005 ◽  
Vol 85 (8) ◽  
pp. 1299-1304 ◽  
Author(s):  
Emil Veliyulin ◽  
Claas van der Zwaag ◽  
Wolfgang Burk ◽  
Ulf Erikson
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Fat Content ◽  
Atlantic Salmon Salmo Salar ◽  
Mobile Nmr

In vivo and in vitro effects of 17α,20β-dihydroxy-4-pregnene-3-one on testicular androgens in atlantic salmon,Salmo salar, mature parr

1997 ◽  
Vol 277 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Efthimia Antonopoulou ◽  
Staffan Jakobsson ◽  
Ian Mayer ◽  
Penny Swanson ◽  
Bertil Borg
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Atlantic Salmon Salmo Salar ◽  
Mature Parr ◽  
In Vitro Effects

Determination of Sexual Maturation Stages of Atlantic Salmon (Salmo salar) Captured at Sea

1981 ◽  
Vol 38 (4) ◽  
pp. 405-413 ◽  
Author(s):  
D. R. Idler ◽  
S. J. Hwang ◽  
L. W. Crim ◽  
D. Reddin
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Gonadosomatic Index ◽  
Gonadal Development ◽  
Maturation Stage ◽  
Female Fish ◽  
Histological Techniques ◽  
Atlantic Salmon Salmo Salar ◽  
Placentia Bay

Radioimmunoassay (RIA) of plasma vitellogenin (Vg), estradiol (E2), 11-ketotestosterone (11-keto), and gonadotropin (GtH), together with histological techniques were evaluated for determination of the maturation stage of Atlantic salmon (Salmo salar) at sea.Male salmon had lower plasma Vg, E2, and higher 11-keto levels and could be distinguished from females several months in advance of spawning. Six female salmon were tagged at sea in 1975 in Placentia Bay and samples of blood taken. When the fish were recovered in rivers the lowest plasma Vg value was 396 μg/mL. This formed the basis of a working hypothesis, that fish with Vg values in excess of 396 μg/mL (~5–6 mo before spawning) will spawn in the year of capture. This hypothesis was supported by the Vg values of an additional 19 tagged females recaptured in Newfoundland and mainland rivers and brackish water off mainland rivers in 1976.Significant correlations between stage of gonadal development and plasma Vg, stage of gonadal development and gonadosomatic index (GSI), stage of gonadal development and plasma E2, plasma Vg and plasma E2, GSI and plasma E2, and between plasma Vg and 11-keto values were found for the female fish. The gonadal development of female fish from Bonavista Bay ranged between oil globule stage and secondary yolk stage. All those females which had reached the primary yolk stage would almost certainly mature and may be considered spawners of the year; on this basis 91% of the females were spawners of the year. Based on the minimum plasma Vg values, at sea, for fish which subsequently returned to the rivers, there were 86% spawners of the year among female salmon taken in Bonavista Bay. Based on plasma Vg levels, spawners of the year ranged from essentially zero in Greenland (fish captured in Greenland during August–November are not spawners of the year, except for the very few that spawn in one Greenland river) to 100% for fish caught in several other fisheries.11-Ketotestosterone was higher in the male fish than in the female fish and there was a correlation between GSI and 11-keto for male fish.The plasma GtH content of fish taken in the sea was extremely low as measured by RIA.Key words: salmon, maturation, migration, radioimmunoassay, vitellogenin, estradiol, gonadotropin, 11-ketotestosterone, histology, gonadosomatic index

scholarly journals Oogenesis in Atlantic salmon (Salmo salar L.) occurs by zonagenesis preceding vitellogenesis in vivo and in vitro

1998 ◽  
Vol 158 (2) ◽  
pp. 259-266 ◽  
Author(s):  
T Celius ◽  
BT Walther
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Polyclonal Antiserum ◽  
Control Mechanisms ◽  
Zona Radiata ◽  
Atlantic Salmon Salmo Salar ◽  
Molecular Components ◽  
Hepatic Synthesis

Fish oogenesis represents pleiotropic cytodifferentiative programs including hepatic synthesis of the molecular components for both the eggshell and the oocytic energy deposits. Both hepatic processes are directly controlled by plasma levels of estradiol (E2), and injected E2 induces both biogenetic processes in prepubertal fish of both sexes. This work compares the temporal pattern of E2-induced biosynthesis of zona radiata proteins (zr-proteins) and vitellogenin in Atlantic salmon (Salmo salar L.) in vivo and in vitro. We monitored the presence of plasma zr-proteins and vitellogenin, using homologous polyclonal antiserum to zr-proteins and a monoclonal antibody to vitellogenin. Zr-proteins were induced by all E2 concentrations (0.001-1.1 mg/kg body weight (bw)) within one week of exposure while vitellogenin was not induced until two weeks post-injection and then only in plasma from fish injected with high E2 concentrations (0.4 mg or 1.1 mg/kg bw). After E2 treatment, hepatocytes isolated from male fish synthesized zr-proteins and vitellogenin in vitro. However, zr-proteins were secreted into the medium two days before vitellogenin, as measured by ELISA. The data indicate a preferential induction of zr-proteins compared with vitellogenin, both with regard to E2 sensitivity and response time to E2 treatment. These findings suggest an obligate sequence in salmon oogenesis. During sexual maturation low E2 levels at first induce only zonagenesis, while increasing levels of E2 subsequently induce both zonagenesis and vitellogenesis. In nature, the interval between zonagenesis and vitellogenesis may, therefore, be considerable. The data suggest new control mechanisms in fish oogenesis.

Development of an in vivo assay to assess attachment of Neoparamoeba sp. (an amphizoic gymnamoeba) to the gills of Atlantic salmon, Salmo salar L.

2007 ◽  
Vol 30 (3) ◽  
pp. 187-190 ◽  
Author(s):  
P B B Crosbie ◽  
M B Adams ◽  
M G Attard ◽  
B F Nowak
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Atlantic Salmon Salmo Salar ◽  
In Vivo Assay

scholarly journals In vitro adaptation of SAV3 in cell culture correlates with reduced in vivo replication capacity and virulence to Atlantic salmon (Salmo salar L.) parr

2015 ◽  
Vol 96 (10) ◽  
pp. 3023-3034 ◽  
Author(s):  
Elin Petterson ◽  
Tz-Chun Guo ◽  
Øyvind Haugland ◽  
Øystein Evensen ◽  
Aase B. Mikalsen
Keyword(s):  
Cell Culture ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Replication Capacity ◽  
Atlantic Salmon Salmo Salar

scholarly journals Regional mtDNA SNP differentiation in European Atlantic salmon (Salmo salar): an assessment of potential utility for determination of natal origin

2012 ◽  
Vol 69 (9) ◽  
pp. 1625-1636 ◽  
Author(s):  
Eric Verspoor ◽  
Sonia Consuegra ◽  
Olafur Fridjonsson ◽  
Sigridur Hjorleifsdottir ◽  
David Knox ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Microsatellite Locus ◽  
Resolving Power ◽  
Restriction Enzyme Digestion ◽  
Potential Utility ◽  
Natal Origin ◽  
Atlantic Salmon Salmo Salar ◽  
Mtdna Snp

Abstract Verspoor, E., Consuegra, S., Fridjonsson, O., Hjorleifsdottir, S., Knox, D., Olafsson, K., Tompsett, S., Wennevik, V., and Garciá de Leániz, C. 2012. Regional mtDNA SNP differentiation in European Atlantic salmon (Salmo salar): an assessment of potential utility for determination of natal origin. – ICES Journal of Marine Science, 69: 1625–1636. The Atlantic salmon, Salmo salar, shows geographically structured differentiation at various classes of molecular genetic variation, among and within river stocks. Nuclear microsatellite locus variation at multiple loci has been exploited for more than a decade as a marker for the continental origin of fish caught at sea in distant-water fisheries. However, a simpler, more cost-effective, but still accurate, assignment can be obtained using a single microsatellite locus in combination with a mitochondrial DNA (mtDNA) single-nucleotide polymorphism (SNP) detected by restriction enzyme digestion. Following on from this, a preliminary study was made of the potential for using mtDNA SNP variation to enhance the resolving power and cost-effectiveness of within-continent assignment of European salmon as determined using microsatellites. Variation in 20 mtDNA regions, encompassing ∼43% of this genome, in 330 salmon from 29 rivers across Europe, was analysed. High levels of inter-individual and inter-river variation were found, as well as evidence of regional differentiation paralleling observed microsatellite differentiation. The observations indicate scope for using mtDNA SNPs along with microsatellites for genetically based assignment of European salmon to region and river of natal origin, but further study is needed.

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