Preparative isolation of neomangiferin and mangiferin fromRhizoma anemarrhenaeby high-speed countercurrent chromatography using ionic liquids as a two-phase solvent system modifier

2010 ◽  
Vol 33 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Lili Xu ◽  
Aifeng Li ◽  
Ailing Sun ◽  
Renmin Liu
Keyword(s):  
Ionic Liquids ◽  
High Speed ◽  
Solvent System ◽  
Countercurrent Chromatography ◽  
Two Phase ◽  
Preparative Isolation

scholarly journals Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Gang Wang ◽  
Dan He ◽  
Fengchun Zhao ◽  
Junqiang Hu ◽  
Yin-Won Lee ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Countercurrent Chromatography ◽  
Macroporous Resin ◽  
Two Phase ◽  
Rice False Smut ◽  
Extraction And Purification ◽  
False Smut ◽  
Optimized Conditions

Abstract Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. Graphical abstract

Preparative Isolation of Anthocyanins from Japanese Purple Sweet Potato (Ipomoea batatas L.) Varieties by High-Speed Countercurrent Chromatography

2010 ◽  
Vol 58 (18) ◽  
pp. 9899-9904 ◽  
Author(s):  
Elyana Cuevas Montilla ◽  
Silke Hillebrand ◽  
Daniela Butschbach ◽  
Susanne Baldermann ◽  
Naoharu Watanabe ◽  
...  
Keyword(s):  
Sweet Potato ◽  
High Speed ◽  
Ipomoea Batatas ◽  
Countercurrent Chromatography ◽  
Preparative Isolation ◽  
Purple Sweet Potato
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