Preparative isolation of caffeoylquinic acid isomers from Kuding tea by salt‐containing aqueous two‐phase extraction and purification by high‐speed countercurrent chromatography

2019 ◽  
Vol 2 (5) ◽  
pp. 170-177
Author(s):  
Dan Liu ◽  
Xinxin Liu ◽  
Xiaozhe Zhang
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography ◽  
Phase Extraction ◽  
Two Phase ◽  
Preparative Isolation ◽  
Caffeoylquinic Acid ◽  
Extraction And Purification ◽  
Aqueous Two Phase Extraction

Extraction and purification of Polyhydroxyalkanoates (PHAs): application of Thermoseparating aqueous two-phase extraction

2017 ◽  
Vol 24 (10) ◽  
Author(s):  
Yoong Kit Leong ◽  
Pau Loke Show ◽  
John Chi-Wei Lan ◽  
Hwei-San Loh ◽  
Yee Jiun Yap ◽  
...  
Keyword(s):  
Phase Extraction ◽  
Two Phase ◽  
Extraction And Purification ◽  
Aqueous Two Phase Extraction

Extraction and Purification of C-Phycocyanin from Spirulina Platensis Employing Aqueous Two Phase Systems

2007 ◽  
Vol 3 (4) ◽  
Author(s):  
AV Narayan ◽  
KSMS Raghavarao
Keyword(s):  
Sodium Sulfate ◽  
Spirulina Platensis ◽  
Potassium Phosphate ◽  
Volume Ratio ◽  
Phase Extraction ◽  
Two Phase ◽  
Extraction And Purification ◽  
Peg 4000 ◽  
Phosphate System ◽  
Aqueous Two Phase Extraction

Aqueous two phase extraction was employed directly to the cell homogenate of Spirulina platensis for the downstream processing of C-phycocyanin. This enables integrating the process steps of cell removal, extraction and concentration into a single unit operation of aqueous two phase extraction. The effect of different parameters such as molecular weight, tie line length, volume ratio and neutral salt (NaCl) were studied employing PEG 4000/potassium phosphate system and PEG 1000/sodium sulfate system to select the best system among the two. For PEG 4000/potassium phosphate system at 13.12% TLL, and volume ratio 0.45, 90.34% yield of phycocyanin with purity of 0.73 was obtained. In the case of PEG 1000 /Na2SO4 system at 34.98 % TLL and volume ratio of 1, 97.62 % yield of phycocyanin with a purity of 0.41 was achieved. Effect of NaCl concentration shows a significant decrease in K of phycocyanin in PEG 1000/Na2SO4 system as compared to PEG 4000/potassium phosphate system. PEG 4000 and potassium phosphate system was found to be better than PEG 1000 and sodium sulfate system for the extraction and purification of C-phycocyanin.

scholarly journals Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Gang Wang ◽  
Dan He ◽  
Fengchun Zhao ◽  
Junqiang Hu ◽  
Yin-Won Lee ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Inhibitory Effect ◽  
Countercurrent Chromatography ◽  
Macroporous Resin ◽  
Two Phase ◽  
Rice False Smut ◽  
Extraction And Purification ◽  
False Smut ◽  
Optimized Conditions

Abstract Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. Graphical abstract

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