Engineering zinc finger protein transcription factors to downregulate the epithelial glycoprotein-2 promoter as a novel anti-cancer treatment

2007 ◽  
Vol 46 (5) ◽  
pp. 391-401 ◽  
Author(s):  
Willemijn M. Gommans ◽  
Pamela M.J. McLaughlin ◽  
Beatrice I. Lindhout ◽  
David J. Segal ◽  
D.J. Wiegman ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Cancer Treatment ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Anti Cancer ◽  
Protein Transcription

scholarly journals 1000. Down-Regulating the Epithelial Glycoprotein-2 Promoter Utilizing Engineered Zinc Finger Protein Transcription Factors

2006 ◽  
Vol 13 ◽  
pp. S385
Author(s):  
Willemijn M. Gommans ◽  
Pamela M.J. McLaughlin ◽  
Robbert Cool ◽  
Beatrice I. Lindhout ◽  
Bert van der Zaal ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein ◽  
Protein Transcription

scholarly journals Regulation of endogenous gene expression using small molecule-controlled engineered zinc-finger protein transcription factors

Gene Therapy ◽  
2007 ◽  
Vol 14 (18) ◽  
pp. 1362-1369 ◽  
Author(s):  
C L Dent ◽  
G Lau ◽  
E A Drake ◽  
A Yoon ◽  
C C Case ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Zinc Finger ◽  
Small Molecule ◽  
Zinc Finger Protein ◽  
Endogenous Gene ◽  
Finger Protein ◽  
Protein Transcription

Gene regulation in planta by plant-derived engineered zinc finger protein transcription factors

2005 ◽  
Vol 57 (3) ◽  
pp. 411-423 ◽  
Author(s):  
Rachel Holmes-Davis ◽  
Guofu Li ◽  
Andrew C. Jamieson ◽  
Edward J. Rebar ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Transcription Factors ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
In Planta ◽  
Finger Protein ◽  
Protein Transcription

scholarly journals Persistent repression of tau in the brain using engineered zinc finger protein transcription factors

2021 ◽  
Vol 7 (12) ◽  
pp. eabe1611
Author(s):  
Susanne Wegmann ◽  
Sarah L. DeVos ◽  
Bryan Zeitler ◽  
Kimberly Marlen ◽  
Rachel E. Bennett ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Messenger Rna ◽  
Zinc Finger Protein ◽  
Neuronal Damage ◽  
Brain Diseases ◽  
Transcriptional Level ◽  
Finger Protein ◽  
Model Of Alzheimer’S Disease ◽  
Protein Transcription

Neuronal tau reduction confers resilience against β-amyloid and tau-related neurotoxicity in vitro and in vivo. Here, we introduce a novel translational approach to lower expression of the tau gene MAPT at the transcriptional level using gene-silencing zinc finger protein transcription factors (ZFP-TFs). Following a single administration of adeno-associated virus (AAV), either locally into the hippocampus or intravenously to enable whole-brain transduction, we selectively reduced tau messenger RNA and protein by 50 to 80% out to 11 months, the longest time point studied. Sustained tau lowering was achieved without detectable off-target effects, overt histopathological changes, or molecular alterations. Tau reduction with AAV ZFP-TFs was able to rescue neuronal damage around amyloid plaques in a mouse model of Alzheimer’s disease (APP/PS1 line). The highly specific, durable, and controlled knockdown of endogenous tau makes AAV-delivered ZFP-TFs a promising approach for the treatment of tau-related human brain diseases.

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