Packed capillary column solvating gas chromatography using neat water mobile phase and flame ionization detection

2001 ◽  
Vol 13 (2) ◽  
pp. 41-47 ◽  
Author(s):  
Naijun Wu ◽  
Qinglin Tang ◽  
J. Andreas Lippert ◽  
Milton L. Lee
Keyword(s):  
Gas Chromatography ◽  
Mobile Phase ◽  
Capillary Column ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Packed Capillary Column ◽  
Flame Ionization ◽  
Solvating Gas Chromatography ◽  
Neat Water

Simultaneous determination of methanol, acetaldehyde, acetone, and ethanol in human blood by gas chromatography with flame ionization detection

2013 ◽  
Vol 33 (1) ◽  
pp. 74-80 ◽  
Author(s):  
J Schlatter ◽  
F Chiadmi ◽  
V Gandon ◽  
P Chariot
Keyword(s):  
Gas Chromatography ◽  
Human Blood ◽  
Capillary Column ◽  
Sodium Tungstate ◽  
Biological Fluids ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Linear Correlations

Background: Methanol, acetaldehyde, acetone, and ethanol, which are commonly used as biomarkers of several diseases, in acute intoxications, and forensic settings, can be detected and quantified in biological fluids. Gas chromatography (GC)–mass spectrometry techniques are complex, require highly trained personnel and expensive materials. Gas chromatographic determinations of ethanol, methanol, and acetone have been reported in one study with suboptimal accuracy. Our objective was to improve the assessment of these compounds in human blood using GC with flame ionization detection. Methods: An amount of 50 µl of blood was diluted with 300 µl of sterile water, 40 µl of 10% sodium tungstate, and 20 µl of 1% sulphuric acid. After centrifugation, 1 µl of the supernatant was inje-cted into the gas chromatograph. We used a dimethylpolysiloxane capillary column of 30 m × 0.25 mm × 0.25 µm. Results: We observed linear correlations from 7.5 to 240 mg/l for methanol, acetaldehyde, and acetone and from 75 to 2400 mg/l for ethanol. Precision at concentrations 15, 60, and 120 mg/l for methanol, acetaldehyde, and acetone and 150, 600, and 1200 mg/ml for ethanol were 0.8–6.9%. Ranges of accuracy were 94.7–98.9% for methanol, 91.2–97.4% for acetaldehyde, 96.1–98.7% for acetone, and 105.5–111.6% for ethanol. Limits of detection were 0.80 mg/l for methanol, 0.61 mg/l for acetaldehyde, 0.58 mg/l for acetone, and 0.53 mg/l for ethanol. Conclusion: This method is suitable for routine clinical and forensic practices.

Study of Endocrine Disruptors in the Sediment of a South Brazilian River

2015 ◽  
Vol 1120-1121 ◽  
pp. 862-866
Author(s):  
Irajá Do Nascimento ◽  
Nathália Christine Vieceli ◽  
Michele Schmitz ◽  
Fernanda Glaeser
Keyword(s):  
Gas Chromatography ◽  
Bisphenol A ◽  
Endocrine Disruptors ◽  
River Sediment ◽  
Flame Ionization Detection ◽  
River Contamination ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Organic Extracts ◽  
Ethylhexyl Phthalate

This study investigated the occurrence of di (2-ethylhexyl) phthalate (DEHP) di-n-butylphthalate (DBP) and bisphenol A (BPA) in river sediment. The samples were collected from three selected points and extracted by sonication, using n-hexane and ethanol. The organic extracts were analyzed by gas chromatography with flame ionization detection (GC/FID). DBP and BPA were not detected. The average concentrations of DEHP range from 0.72 (±0.04) to 27.90 (± 3.05) ng g-1 of sediment. The best solvent for the extractions was n-hexane. However ethanol also shows good extraction yields of DEHP. These results showed an important anthropic contribution for the river contamination by endocrine disruptors.

Analysis of light weight fractions of coal-based crude oil by gas chromatography combined with mass spectroscopy and flame ionization detection

Fuel ◽  
2019 ◽  
Vol 241 ◽  
pp. 392-401 ◽  
Author(s):  
Wen-ying Li ◽  
Wei Wang ◽  
Hai Mu ◽  
Wang Li ◽  
Cui-ping Ye ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Crude Oil ◽  
Mass Spectroscopy ◽  
Flame Ionization Detection ◽  
Light Weight ◽  
Ionization Detection ◽  
Flame Ionization

scholarly journals Quantitative Analysis of Urinary O-Cresol by Gas Chromatography - Flame Ionization Detection for the Monitoring of Population Exposed to Toluene

2018 ◽  
Vol 3 (2) ◽  
pp. 1-5
Author(s):  
Julio Navoni ◽  
Adriana Ridolfi ◽  
Mónica Olivera ◽  
Gloria Álvarez ◽  
Edda Villaamil Lepori
Keyword(s):  
Gas Chromatography ◽  
Quantitative Analysis ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization

The secretion of ibuprofen metabolites interferes with the capillary chromatography of urinary homovanillic acid and 4-hydroxy-3-methoxymandelic acid in neuroblastoma diagnosis

2005 ◽  
Vol 43 (2) ◽  
Author(s):  
Isabella Levreri ◽  
Ubaldo Caruso ◽  
Franca Deiana ◽  
Antonella Buoncompagni ◽  
Bruno De Bernardi ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Capillary Gas Chromatography ◽  
Homovanillic Acid ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Catecholamine Metabolism ◽  
Flame Ionization Detection ◽  
Trimethylsilyl Ester ◽  
Ionization Detection ◽  
Flame Ionization

AbstractNeuroblastoma is the most common extracranial solid tumor in children. Abnormal secretion of catechol-amines in tissues and body fluids allows for the differential diagnosis of neuroblastoma from other neoplasms and its distinction from non-neoplastic inflammatory diseases. This is achieved by assaying homovanillic acid and 4-hydroxy-3-methoxymandelic acid, the catabolites of catecholamine metabolism. In the course of an evaluation of children with suspected neuroblastoma, homovanillic acid and 4-hydroxy-3-methoxymandelic acid were analyzed in urine samples by capillary gas chromatography with flame ionization detection after extraction and derivatization of these compounds as trimethylsilyl derivatives. In three urine samples a significant increase in biogenic amines was observed, but these results were not confirmed by thin-layer chromatography. Patient history revealed that these children had been treated with ibuprofen, an analgesic and anti-inflammatory drug. To verify how ibuprofen or its metabolites may have interfered with capillary gas chromatography with flame ionization detection, we analyzed the same samples by capillary gas chromatography-mass spectrometry. In urine samples from patients on the drug, the presence of a peak identified as the trimethylsilyl ester of hydroxyibuprofen, which had the same retention time as 4-hydroxy-3-methoxymandelic acid, was found to interfere with the capillary gas chromatography with flame ionization detection analysis of the metabolite. This interference must be taken into account during the laboratory diagnosis of neuroblastoma.

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