Effect of pancreatic allografts on vascular basement membrane thickness in the diabetic Rat

1. Microvascular blood flow responses to injury and capillary ultrastructure were assessed by laser Doppler flowmetry and detailed light and electron microscopy respectively in skin biopsied from 28 patients with insulin-dependent diabetes and 17 control subjects. 2. The hyperaemic response induced by biopsy (P < 0.001) and heating to 44°C (P < 0.001) was significantly lower in the diabetic patients and showed progressive impairment with the severity of complications (P < 0.001). 3. Skin capillary basement membrane thickness was significantly increased in the diabetic patients (P < 0.001) and also increased with the severity of complications (P < 0.002). Both the luminal area (P < 0.001) and the endothelial cell outer perimeter (P < 0.002), measures of luminal and capillary size, respectively, were significantly reduced in all diabetic patients. 4. Basement membrane thickness was related significantly to the impaired hyperaemic response to both biopsy (P < 0.01) and thermal injury (P < 0.01). 5. Our findings support the hypothesis that structural abnormalities, which are characterized by an early reduction in capillary size and later thickening of basement membrane, form an important mechanism for the impaired hyperaemic response in diabetic patients.

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The Relationship of Reticular Basement Membrane Thickness to Airway Wall Remodeling in Asthma

American Journal of Respiratory and Critical Care Medicine ◽

10.1164/rccm.2108069 ◽

2002 ◽

Vol 166 (12) ◽

pp. 1590-1595 ◽

Cited By ~ 100

Author(s):

Alan L. James ◽

Peta S. Maxwell ◽

Gladys Pearce-Pinto ◽

John G. Elliot ◽

Neil G. Carroll

Keyword(s):

Basement Membrane ◽

Membrane Thickness ◽

Airway Wall ◽

Basement Membrane Thickness ◽

Relationship Of ◽

The Relationship

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Dependency of Muscle Capillary Basement Membrane Thickness on the Duration of Diabetes

Diabetes Care ◽

10.2337/diacare.2.6.472 ◽

1979 ◽

Vol 2 (6) ◽

pp. 472-478 ◽

Cited By ~ 13

Author(s):

E. A. Siess ◽

H. E. Nathke ◽

T. Dexel ◽

M. Haslbeck ◽

H. Mehnert ◽

...

Keyword(s):

Basement Membrane ◽

Membrane Thickness ◽

Basement Membrane Thickness ◽

Duration Of Diabetes ◽

Capillary Basement Membrane

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Effects of Aminoguanidine on Glomerular Basement Membrane Thickness and Anionic Charge in a Diabetic Rat Model

International Journal of Experimental Diabetes Research ◽

10.1155/edr.2001.225 ◽

2001 ◽

Vol 2 (3) ◽

pp. 225-232 ◽

Cited By ~ 4

Author(s):

Dilek Gogas Yavuz ◽

Halil Önder Ersöz ◽

Mürvet Tuncel ◽

Mustafa F. Sargon ◽

Belgin Küçükkaya ◽

...

Keyword(s):

Heparan Sulfate ◽

Tap Water ◽

Diabetic Rats ◽

Urinary Albumin ◽

Diabetic Rat ◽

Membrane Thickness ◽

Water Control ◽

Basement Membrane Thickness ◽

Water Group ◽

Anionic Charge

We investigated the effect of aminoguanidine (AG) administration on GBM thickness, glomerular heparan sulfate (HS) content, and urinary albumin and HS excretion in diabetic rats. After induction of diabetes, female Wistar rats were divided into 2 groups: Group AGDM (n=11) received 1g/L aminoguanidine bicarbonate in drinking water, group DC (n=12) was given only tap water. Control rats received AG (group AGH, n=8) or tap water (group HC, n=8). At the end of a period of 8 weeks, urinary albumin and glycosaminoglycan (GAG) excretion was detected. GBM heparan sulfate distribution and count was determined under the electron microscope. The AGDM group had lower urinary albumin and GAG excretion than diabetic controls. GBM thickness was increased in diabetic rats compared to groups of AGDM and HC. In AGDM group alcian blue stained particle distribution and count in the GBM was similar to healthy controls. In conclusion AG prevents the decrease of anionic charged molecules in the GBM and GBM thickening. This can be one of the mechanisms by which AG decreases albuminuria in diabetic rats.

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Influences of physical conditioning and deconditioning on coronary vasculature of dogs

Journal of Applied Physiology ◽

10.1152/jappl.1978.45.4.619 ◽

1978 ◽

Vol 45 (4) ◽

pp. 619-625 ◽

Cited By ~ 67

Author(s):

H. L. Wyatt ◽

J. Mitchell

Keyword(s):

Basement Membrane ◽

Capillary Density ◽

Cross Sectional Area ◽

Membrane Thickness ◽

Physical Conditioning ◽

Sectional Area ◽

Circumflex Artery ◽

Cross Sectional ◽

Coronary Vasculature ◽

Basement Membrane Thickness

To investigate the effects of physical conditioning and deconditioning on the coronary vasculature, eight dogs were exercised by treadmill running. Five dogs were deconditioned by confinement in cages following the conditioning period. A technique was developed and validated for measuring circumflex coronary artery diameter from magnified projections of standardized coronary angiograms. Myocardial capillary density, perimeter, and basement membrane thickness were determined from electron microscopy of serial ventricular septal biopsy samples. Physical conditioning caused a small but statistically significant increase in cross-sectional area of the circumflex artery. Although physical conditioning caused no statistically significant changes in the myocardial capillaries, trends were apparent for increases in density and perimeter of myocardial capillaries and a decrease in basement membrane thickness. Physical deconditioning caused statistically significant reductions in cross-sectional area of the circumflex artery and in myocardial capillary density but little change in perimeter or basement membrane thickness of myocardial capillaries. The results suggest that physical conditioning may be associated with an improvement in coronary vascular capacity which may regress rapidly with deconditioning.

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