Cis-acting elements (E-box and NBE) in the promoter region of three maternal genes (Histone H1oo, Nucleoplasmin 2, andZygote Arrest 1) are required for oocyte-specific gene expression in the mouse

The molecular basis of adipocyte-specific gene expression is not well understood. We have previously identified a 518-bp enhancer from the adipocyte P2 gene that stimulates adipose-specific gene expression in both cultured cells and transgenic mice. In this analysis of the enhancer, we have defined and characterized a 122-bp DNA fragment that directs differentiation-dependent gene expression in cultured preadipocytes and adipocytes. Several cis-acting elements have been identified and shown by mutational analysis to be important for full enhancer activity. One pair of sequences, ARE2 and ARE4, binds a nuclear factor (ARF2) present in extracts derived from many cell types. Multiple copies of these elements stimulate gene expression from a minimal promoter in preadipocytes, adipocytes, and several other cultured cell lines. A second pair of elements, ARE6 and ARE7, binds a separate factor (ARF6) that is detected only in nuclear extracts derived from adipocytes. The ability of multimers of ARE6 or ARE7 to stimulate promoter activity is strictly adipocyte specific. Mutations in the ARE6 sequence greatly reduce the activity of the 518-bp enhancer. These data demonstrate that several cis- and trans-acting components contribute to the activity of the adipocyte P2 enhancer and suggest that ARF6, a novel differentiation-dependent factor, may be a key regulator of adipogenic gene expression.

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Nonlinear partial differential equations and applications: Pineal expression-promoting element (PIPE), a cis-acting element, directs pineal-specific gene expression in zebrafish

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.232444199 ◽

2002 ◽

Vol 99 (24) ◽

pp. 15456-15461 ◽

Cited By ~ 33

Author(s):

Y. Asaoka ◽

H. Mano ◽

D. Kojima ◽

Y. Fukada

Keyword(s):

Gene Expression ◽

Partial Differential Equations ◽

Differential Equations ◽

Nonlinear Partial Differential Equations ◽

Specific Gene ◽

Partial Differential ◽

Cis Acting ◽

Specific Gene Expression

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Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression.

Molecular and Cellular Biology ◽

10.1128/mcb.12.3.1202 ◽

1992 ◽

Vol 12 (3) ◽

pp. 1202-1208 ◽

Cited By ~ 83

Author(s):

R A Graves ◽

P Tontonoz ◽

B M Spiegelman

Keyword(s):

Gene Expression ◽

Cultured Cells ◽

Mutational Analysis ◽

Cell Types ◽

Specific Gene ◽

Enhancer Activity ◽

Cis Acting ◽

Specific Gene Expression ◽

Nuclear Extracts ◽

Adipogenic Gene

The molecular basis of adipocyte-specific gene expression is not well understood. We have previously identified a 518-bp enhancer from the adipocyte P2 gene that stimulates adipose-specific gene expression in both cultured cells and transgenic mice. In this analysis of the enhancer, we have defined and characterized a 122-bp DNA fragment that directs differentiation-dependent gene expression in cultured preadipocytes and adipocytes. Several cis-acting elements have been identified and shown by mutational analysis to be important for full enhancer activity. One pair of sequences, ARE2 and ARE4, binds a nuclear factor (ARF2) present in extracts derived from many cell types. Multiple copies of these elements stimulate gene expression from a minimal promoter in preadipocytes, adipocytes, and several other cultured cell lines. A second pair of elements, ARE6 and ARE7, binds a separate factor (ARF6) that is detected only in nuclear extracts derived from adipocytes. The ability of multimers of ARE6 or ARE7 to stimulate promoter activity is strictly adipocyte specific. Mutations in the ARE6 sequence greatly reduce the activity of the 518-bp enhancer. These data demonstrate that several cis- and trans-acting components contribute to the activity of the adipocyte P2 enhancer and suggest that ARF6, a novel differentiation-dependent factor, may be a key regulator of adipogenic gene expression.

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Proximal promoter region of the wheat histone H3 gene confers S phase-specific gene expression in transformed rice cells

Plant Molecular Biology ◽

10.1007/bf00019303 ◽

1993 ◽

Vol 23 (3) ◽

pp. 553-565 ◽

Cited By ~ 15

Author(s):

Norihiro Ohtsubo ◽

Takuya Nakayama ◽

Rie Terada ◽

Ko Shimamoto ◽

Masaki Iwabuchi

Keyword(s):

Gene Expression ◽

Promoter Region ◽

Histone H3 ◽

S Phase ◽

Proximal Promoter ◽

Specific Gene ◽

Proximal Promoter Region ◽

Specific Gene Expression ◽

Histone H3 Gene

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Characterization of cis-acting sequences regulating root-specific gene expression in tobacco.

The Plant Cell ◽

10.1105/tpc.3.4.371 ◽

1991 ◽

Vol 3 (4) ◽

pp. 371-382 ◽

Cited By ~ 90

Author(s):

Y T Yamamoto ◽

C G Taylor ◽

G N Acedo ◽

C L Cheng ◽

M A Conkling

Keyword(s):

Gene Expression ◽

Specific Gene ◽

Cis Acting ◽

Specific Gene Expression

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Characterization of cis-Acting Sequences Regulating Root-Specific Gene Expression in Tobacco

The Plant Cell ◽

10.2307/3869212 ◽

1991 ◽

Vol 3 (4) ◽

pp. 371 ◽

Cited By ~ 1

Author(s):

Yuri T. Yamamoto ◽

Christopher G. Taylor ◽

Gregoria N. Acedo ◽

Chi-Lien Cheng ◽

Mark A. Conkling

Keyword(s):

Gene Expression ◽

Specific Gene ◽

Cis Acting ◽

Specific Gene Expression

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Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis

Development ◽

10.1242/dev.109.3.705 ◽

1990 ◽

Vol 109 (3) ◽

pp. 705-713 ◽

Cited By ~ 2

Author(s):

D. Twell ◽

J. Yamaguchi ◽

S. McCormick

Keyword(s):

Gene Expression ◽

Promoter Region ◽

Male Gametophyte ◽

Regulation Of Gene Expression ◽

Gus Activity ◽

Transgenic Tomato ◽

Specific Gene ◽

Gene Promoters ◽

Specific Expression ◽

Specific Gene Expression

To investigate the regulation of gene expression during male gametophyte development, we analyzed the promoter activity of two different genes (LAT52 and LAT59) from tomato, isolated on the basis of their anther-specific expression. In transgenic tomato, tobacco and Arabidopsis plants containing the LAT52 promoter region fused to the beta-glucuronidase (GUS) gene, GUS activity was restricted to pollen. Transgenic tomato, tobacco and Arabidopsis plants containing the LAT59 promoter region fused to GUS also showed very high levels of GUS activity in pollen. However, low levels of expression of the LAT59 promoter construct were also detected in seeds and roots. With both constructs, the appearance of GUS activity in developing anthers was correlated with the onset of microspore mitosis and increased progressively until anthesis (pollen shed). Our results demonstrate co-ordinate regulation of the LAT52 and LAT59 promoters in developing microspores and suggest that the mechanisms that regulate pollen-specific gene expression are evolutionarily conserved.

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