Electron microscopic localization of cell-surface markers in tissue sections using monoclonal and gold-conjugated antibodies

1986 ◽  
Vol 148 (1) ◽  
pp. 29-34 ◽  
Author(s):  
N. Rooney ◽  
C. Day ◽  
T. Gray ◽  
J. C. E. Underwood
Keyword(s):  
Cell Surface ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Electron Microscopic ◽  
Tissue Sections ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

Organization of stigma surface components in Brassica: a cytochemical study

1986 ◽  
Vol 82 (1) ◽  
pp. 203-216
Author(s):  
T. Gaude ◽  
C. Dumas
Keyword(s):  
Cell Surface ◽  
Adenylate Cyclase ◽  
Enzyme Activities ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Electron Microscopic ◽  
Cytochemical Study ◽  
Stigma Surface ◽  
Specific Labelling

The nature of secretion products forming the pellicle of the dry-type stigma surface has been investigated in Brassica by an electron-microscopic cytochemical study. In order to determine whether the pellicle is organized like a membrane, we used a series of cytochemical methods to visualize cell membranes. Three groups of techniques presenting different degrees of specificity in terms of cell surface markers have been used: non-specific labelling of cell-surface components, characterization of glycoconjugates and localization of enzymic activities. We demonstrated that the pellicle of Brassica stigmas, although not possessing a trilamellar structure, presents numerous characteristics encountered in biological membranes. In particular, it possesses cytochemically demonstrable enzyme activities, including ATPase and adenylate cyclase, whose role remains to be elucidated in relation to the pollen-stigma interactions.

The electron microscopic localization of nephrotoxic antibodies in isolated glomeruli

Pathology ◽  
1976 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
I.P. McCausland ◽  
R.N. Seelye ◽  
J.B. Gavin ◽  
P.B. Herdson
Keyword(s):  
Electron Microscopic ◽  
Isolated Glomeruli ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

Electron Microscopic Localization of Antigen in Experimental Bronchoconstriction in Guinea Pigs

CHEST Journal ◽  
1973 ◽  
Vol 63 (4) ◽  
pp. 44S ◽  
Author(s):  
J.B. Richardson ◽  
J.C. Hogg ◽  
T. Bouchard ◽  
D.L. Hall
Keyword(s):  
Guinea Pigs ◽  
Electron Microscopic ◽  
Microscopic Localization ◽  
Electron Microscopic Localization
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