Cell surface markers for immunophenotyping human pluripotent stem cell-derived cardiomyocytes

2021 ◽  
Author(s):  
Kenneth R. Boheler ◽  
Ellen Ngar-Yun Poon
Keyword(s):  
Stem Cell ◽  
Cell Surface ◽  
Pluripotent Stem Cell ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Human Pluripotent Stem Cell

Metabolic glycan labeling-assisted discovery of cell-surface markers for primary neural stem and progenitor cells

2018 ◽  
Vol 54 (43) ◽  
pp. 5486-5489
Author(s):  
Qing-Ran Bai ◽  
Lu Dong ◽  
Yi Hao ◽  
Xing Chen ◽  
Qin Shen
Keyword(s):  
Mass Spectrometry ◽  
Stem Cell ◽  
Cell Surface ◽  
Progenitor Cells ◽  
Neural Stem Cell ◽  
Surface Markers ◽  
Metabolic Labeling ◽  
Cell Surface Markers ◽  
Stem And Progenitor Cells

Metabolic labeling with azidosugars in a neural stem cell (NSC)-enriched endothelial coculture followed by mass-spectrometry profiling identifies sialoglycoproteins on NSCs.

Identification of Human Embryonic Stem Cell Surface Markers by Combined Membrane-Polysome Translation State Array Analysis and Immunotranscriptional Profiling

Stem Cells ◽  
2009 ◽  
Vol 27 (10) ◽  
pp. 2446-2456 ◽  
Author(s):  
Gabriel Kolle ◽  
Mirabelle Ho ◽  
Qi Zhou ◽  
Hun S. Chy ◽  
Keerthana Krishnan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Surface ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Array Analysis ◽  
Translation State

scholarly journals Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

2020 ◽  
Author(s):  
Alexander Patera Nugraha ◽  
Fedik Abdul Rantam ◽  
Ida Bagus Narmada ◽  
Diah Savitri Ernawati ◽  
Igo Syaiful Ihsan
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Cell Surface ◽  
Osteogenic Differentiation ◽  
Oryctolagus Cuniculus ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Alizarin Red ◽  
Regenerative Dentistry

Abstract Objective This study aims to confirm whether the GDMSCs isolated from rabbit’s (Oryctolagus cuniculus) gingiva are mesenchymal stem cells (MSCs). Materials and Methods This study design was partly quasi-experimental with an observational design. GDMSCs were isolated from the gingiva of healthy male rabbits (O. cuniculus) (n = 2), 6 months old, and 3 to 5 kg of body weight. The specific cell surface markers of MSCs; clusters of differentiation (CD), namely, CD44, CD73, CD90, CD105, and CD200 expressions; and hematopoietic stem cell surface markers CD34 and CD45 were examined using flow cytometry and immunohistochemistry with immunofluorescence. The osteogenic differentiation of isolated GDMSCs was examined using alizarin red staining. Results GDMSCs in the fourth passage showed a spindle-like formation and fibroblast-like cells that attached to the base of the culture plate. GDMSCs were MSCs that positively expressed CD44, CD73, CD90, CD105, and CD200 but did not express CD34 and CD45 when examined using flow cytometry and immunohistochemical analysis. GDMSCs had osteogenic differentiation confirmed by calcified deposits in vitro with a red–violet and brownish color after alizarin red staining. Conclusion GDMSCs isolated from the rabbits (O. cuniculus) were confirmed as MSCs in vitro documented using immunohistochemistry and flow cytometry. GDMSCs can differentiate into osteogenic lineage in vitro that may be suitable for regenerative dentistry.

scholarly journals A Human Pluripotent Stem Cell Surface N-Glycoproteome Resource Reveals Markers, Extracellular Epitopes, and Drug Targets

2014 ◽  
Vol 3 (1) ◽  
pp. 185-203 ◽  
Author(s):  
Kenneth R. Boheler ◽  
Subarna Bhattacharya ◽  
Erin M. Kropp ◽  
Sandra Chuppa ◽  
Daniel R. Riordon ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Surface ◽  
Drug Targets ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell

Breast cancer stem cells: A novel therapy for breast cancer.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e13030-e13030
Author(s):  
Soma Mukhopadhyay ◽  
Sudeshna Gangopadhyay ◽  
Swati Dasgupta ◽  
Saubhik Sengupta ◽  
Ujjal Kanti Ray ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cell Surface ◽  
Surface Markers ◽  
Breast Cancer Stem Cells ◽  
Cell Surface Markers ◽  
Primary Tumors ◽  
Free Survival

e13030 Background: Simple BRCA screening is insufficient for ‘event-free survival’ as breast cancer is clinically and pathologically an extremely heterogeneous disease. Targeting Breast Cancer Stem Cells (BCSCs) present in bone marrow and breast tissues is a lucrative alternative. Identification of BCSCs is salient aspect of our research. Invasive and mesenchymal property of BCSCs with CD44+/CD24low/ALDH1+ phenotype has made them a promising target for eliminating metastatic capacity of primary tumors. We hypothesize that ability to therapeutically attack stem cell hinges upon identifying unique targets like cell surface markers and this will decide development of specific target therapies. Methods: A total of 10 early chemo-naive patients with biopsy proven triple-negative metastatic breast cancer in the age group of 18-36 yr.s (mean age 28 yr.s) were selected randomly and tested for CD44/CD24 cell surface markers following immunosorting using magnetic cell sorter and immunophenotyping by flowcytometric analysis. Isolated BCSCs were cultured for in vitro drug sensitivity towards platinum, anthracycline and docetaxel. Correlation was drawn between cell differentiation, % of stem cells and drug response. Accordingly chemotherapy was designed for a particular patient. % of BCSCs in pre- and post-chemotherapeutic condition was further compared. Results: We have detected BCSCs in 90% of cases. Among positive samples, 89% patients showed platinum sensitivity and rest were found to be anthracycline sensitive. No sensitivity to docetaxel was observed. In lieu of this, cisplatin was applied in vivo and % of BCSCs came down to 6.58% from initial 11.16% (for a representative case). Conclusions: Thus primary aim to target BCSCs at the onset of tumors in breast cancer patients to control metastasis and relapse of disease was somewhat obtained. We further plan to correlate ratio of selected markers present in patients in pre- and post-chemotherapeutic condition with time to recurrence, mortality, morbidity and progression-free survival. Finally, if no BCSCs prevail after chemotherapy, then patients would be kept under observation and if traces are found, we would proceed to targeted therapy trial like PARP inhibitor or autologous stem cell replacement.

Examination of Adipose Tissue-derived Mesenchymal Stem Cell Surface Markers in a Hypoxic Environment

2020 ◽  
Vol 14 (5) ◽  
pp. 325-331
Author(s):  
Gulsemin Çiçek ◽  
Emine Utlu Ozen ◽  
Fatma Oz Bagcı ◽  
Selcuk Duman ◽  
T. Murad Aktan ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Surface ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Hypoxic Environment

The quest of cell surface markers for stem cell therapy

2020 ◽  
Author(s):  
Anna Meyfour ◽  
Sara Pahlavan ◽  
Mehdi Mirzaei ◽  
Jeroen Krijgsveld ◽  
Hossein Baharvand ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Surface ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Surface Markers ◽  
Cell Surface Markers

scholarly journals Developmental stage-specific gene expression distinguishes the murine γδ T-cell lineage

2019 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
T Cell ◽  
Cell Surface ◽  
Γδ T Cells ◽  
Specific Gene ◽  
Intermediate Cell ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Γδ T Cell

It remains unclear whether γδ T-cell fate is instructed or reinforced by the γδTCR. Prospective isolation of a multi-potent γδ cell progenitor (“the γδ stem cell”) and precisely assessing when the γδTCR is displayed in relation to developmental emergence of multi-lineage reconstitution could resolve this question but there is a lack of reliable cell surface markers for the γδ stem cell. The double-negative 3a stage (“DN3a”) is a critical intermediate cell-state during the transition from non-committed T-lymphocyte progenitor to mature γδ or 𝛂β T-cell, as γδTcr rearrangements and β-selection can theoretically temporally co-exist during this time in lymphocyte development. In this study I compared the transcriptomes of murine stage DN3a thymocytes to that of γδ T-cells (Tγδ) to illustrate the key transcriptional differences during this developmental transition. The analyses here revealed a series of cell surface markers, transcription factors, cytokine receptors, cell adhesion genes and non-coding RNA that represent some of the major transcriptional differences between γδ T-cells and the cells that they might arise from.

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