Qualitative and Quantitative Analysis ofPotentilla fulgensRoots by NMR, Matrix-assisted Laser Desorption/Ionisation with Time-of-Flight MS, Electrospray Ionisation MS/MS and HPLC/UV

2014 ◽  
Vol 26 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Alka Choudhary ◽  
Manukonda Radhika ◽  
Anupam Chatterjee ◽  
Uttam Chand Banerjee ◽  
Inder Pal Singh
Keyword(s):  
Quantitative Analysis ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Electrospray Ionisation ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Critical factors determining the quantification capability of matrix-assisted laser desorption/ionization– time-of-flight mass spectrometry

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150371 ◽  
Author(s):  
Chia-Chen Wang ◽  
Yin-Hung Lai ◽  
Yu-Meng Ou ◽  
Huan-Tsung Chang ◽  
Yi-Sheng Wang
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Maldi Tof Ms ◽  
Desorption Ionization ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Quantitative analysis with mass spectrometry (MS) is important but challenging. Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) MS offers superior sensitivity, resolution and speed, but such techniques have numerous disadvantages that hinder quantitative analyses. This review summarizes essential obstacles to analyte quantification with MALDI-TOF MS, including the complex ionization mechanism of MALDI, sensitive characteristics of the applied electric fields and the mass-dependent detection efficiency of ion detectors. General quantitative ionization and desorption interpretations of ion production are described. Important instrument parameters and available methods of MALDI-TOF MS used for quantitative analysis are also reviewed. This article is part of the themed issue ‘Quantitative mass spectrometry’.

Quantitative Analysis of Peptides by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry

1993 ◽  
Vol 47 (8) ◽  
pp. 1091-1092 ◽  
Author(s):  
Arkady Ia. Gusev ◽  
William R. Wilkinson ◽  
Andrew Proctor ◽  
David M. Hercules
Keyword(s):  
Quantitative Analysis ◽  
Laser Energy ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Sample Surface ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Relative Standard ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Quantitative analysis of peptides using internal standards was carried out with the use of matrix-assisted laser desorption/ionization (MALDI) on a time-of-flight mass spectrometer in a ferulic acid matrix. Spectra were collected and averaged over several areas of the sample surface at constant laser energy, and a defocused beam was used to improve reproducibility. A calibration curve was plotted, showing a correlation coefficient of 0.992 and a relative standard deviation of 1.83%. The accuracy and precision of this technique were sensitive to the crystal structure of the matrix and saturation effects of the instrument's detector.

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