A combining signal amplification of atom transfer radical polymerization and redox polymerization for visual biomolecules detection

Poly(methyl methacrylate)-b-poly(N-isopropylacrylamide) [PMMA-b-PNIPAM] block copolymers were obtained by a combination of redox polymerization and atom transfer radical polymerization (ATRP) methods in two steps. For this purpose, PMMA macroinitator (ATRP-macroinitiator) was synthesized by redox polymerization of methyl methacrylate and 3-bromo-1-propanol using Ce(NH4)2(NO3)6 as a catalyst. The synthesis of PMMA-b-PNIPAM block copolymers was carried out by means of ATRP of ATRP-macroinitiator and NIPAM at 60 °C. The block copolymers were obtained in high yield and high molecular weight. The characterization of products was accomplished by using multi instruments and methods such as nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, gel permeation chromatography, and thermogravimetric analysis.

Download Full-text

High sensitive electrochemical methamphetamine detection in serum and urine via atom transfer radical polymerization signal amplification

Talanta ◽

10.1016/j.talanta.2021.123026 ◽

2021 ◽

pp. 123026

Author(s):

Haobo Sun ◽

Jingliang Liu ◽

Yunliang Qiu ◽

Jinming Kong ◽

Xueji Zhang

Keyword(s):

Atom Transfer Radical Polymerization ◽

Radical Polymerization ◽

Signal Amplification ◽

Atom Transfer ◽

Serum And Urine

Download Full-text

Electrochemiluminescence immunosensor for cytokeratin fragment antigen 21-1 detection using electrochemically mediated atom transfer radical polymerization

Microchimica Acta ◽

10.1007/s00604-020-04677-x ◽

2021 ◽

Vol 188 (4) ◽

Author(s):

Lihe Jian ◽

Xiaolan Wang ◽

Lulu Hao ◽

Yanju Liu ◽

Huaixia Yang ◽

...

Keyword(s):

Lung Cancer ◽

Atom Transfer Radical Polymerization ◽

Radical Polymerization ◽

Signal Amplification ◽

Limit Of Detection ◽

Tumor Biomarker ◽

Atom Transfer ◽

Sandwich Type ◽

Diagnosis Of Lung Cancer ◽

Antigen Antibody

AbstractThe cytokeratin fragment antigen 21-1 (CYFRA 21-1) protein is a critical tumor biomarker tightly related to non-small cell lung cancer (NSCLC). Herein, we prepared an effective electrochemiluminescence (ECL) immunosensor for CYFRA 21-1 detection using electrochemically mediated atom transfer radical polymerization (eATRP). The CYFRA 21-1 antigen was fixed on the electrode surface by constructing a sandwich type antibody-antigen-antibody immune system. The sensitivity of ECL was improved by using the eATRP reaction. In this method, eATRP was applied to CYFRA 21-1 detection antibody with N-acryloyloxysuccinimide as functional monomer. This is the first time that ECL and eATRP signal amplification technology had been combined. Under the optimized testing conditions, the immunosensor showed a good linear relation in the range from 1 fg mL−1 to 1 μg mL−1 at a limit of detection of 0.8 fg mL−1 (equivalent to ~ 134 molecules in a 10 μL sample). The ECL immunosensing system based on eATRP signal amplification technology provided a new way for rapid diagnosis of lung cancer by detecting CYFRA 21-1. Graphical abstract

Download Full-text