Comprehensive identification of erythrocyte membrane protein deficiency by 2D gel electrophoresis based proteomic analysis in hereditary elliptocytosis and spherocytosis

2012 ◽  
Vol 6 (7-8) ◽  
pp. 403-411 ◽  
Author(s):  
Duygu Ozel Demiralp ◽  
Selen Peker ◽  
Burhan Turgut ◽  
Nejat Akar
Keyword(s):  
Membrane Protein ◽  
Erythrocyte Membrane ◽  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Protein Deficiency ◽  
2D Gel Electrophoresis ◽  
Erythrocyte Membrane Protein ◽  
Hereditary Elliptocytosis ◽  
2D Gel

scholarly journals Hereditary elliptocytosis with protein band 4.1 deficiency in the dog

Blood ◽  
1983 ◽  
Vol 61 (2) ◽  
pp. 373-377 ◽  
Author(s):  
JE Smith ◽  
K Moore ◽  
M Arens ◽  
GA Rinderknecht ◽  
A Ledet
Keyword(s):  
Animal Model ◽  
Membrane Protein ◽  
Erythrocyte Membrane ◽  
Protein Band ◽  
Erythrocyte Deformability ◽  
Membrane Stability ◽  
Osmotic Sensitivity ◽  
Hereditary Elliptocytosis ◽  
Membrane Cytoskeleton ◽  
Glutathione Deficiency

Abstract A dog with persistent elliptocytosis was studied. The dog had membrane protein band 4.1 deficiency, microcytosis, shortened erythrocyte lifespan, increased osmotic sensitivity, and a mild glutathione deficiency. Erythrocyte deformability and membrane stability were adversely effected. The dog's parents had decreased band 4.1, decreased stability, and some elliptocytosis. This disorder in dogs closely resembles human patients with band 4.1 deficiency and should provide a valuable animal model to study the erythrocyte membrane cytoskeleton.

Identification of nuclei associated proteins by 2D-gel electrophoresis and mass spectrometry

2001 ◽  
Vol 109 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Jonas Bergquist ◽  
Johan Gobom ◽  
Anders Blomberg ◽  
Peter Roepstorff ◽  
Rolf Ekman
Keyword(s):  
Mass Spectrometry ◽  
Gel Electrophoresis ◽  
2D Gel Electrophoresis ◽  
Associated Proteins ◽  
2D Gel
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