scholarly journals Fluorescent probe for high-throughput screening of membrane protein expression

2013 ◽  
Vol 22 (8) ◽  
pp. 1124-1132 ◽  
Author(s):  
A. E. Backmark ◽  
N. Olivier ◽  
A. Snijder ◽  
E. Gordon ◽  
N. Dekker ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Fluorescent Probe ◽  
High Throughput ◽  
High Throughput Screening ◽  
Membrane Protein Expression

Generation and evaluation of mammalian secreted and membrane protein expression libraries for high-throughput target discovery

2011 ◽  
Vol 79 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Tadas Panavas ◽  
Jin Lu ◽  
Xuesong Liu ◽  
Ann-Marie Winkis ◽  
Gordon Powers ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
High Throughput ◽  
Target Discovery ◽  
Membrane Protein Expression ◽  
Expression Libraries

High‐throughput Cell‐free Screening of Eukaryotic Membrane Protein Expression in Lipidic Mimetics

2021 ◽  
Author(s):  
Renato Bruni ◽  
Aisha Laguerre ◽  
Anna‐Maria Kaminska ◽  
Sean McSweeney ◽  
Wayne A. Hendrickson ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
High Throughput ◽  
Membrane Protein Expression ◽  
Eukaryotic Membrane Protein

scholarly journals A Near‐Infrared Organic Fluorescent Probe for Broad Applications for Blood Vessels Imaging by High‐Throughput Screening via 3D‐Blood Vessel Models (Small Methods 8/2021)

Small Methods ◽  
2021 ◽  
Vol 5 (8) ◽  
pp. 2170036
Author(s):  
Muhammad Asri Abdul Sisak ◽  
Fiona Louis ◽  
Ichio Aoki ◽  
Sun Hyeok Lee ◽  
Young‐Tae Chang ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Fluorescent Probe ◽  
High Throughput ◽  
High Throughput Screening ◽  
Near Infrared

Quantitative Membrane Protein Expression at the Blood–Brain Barrier of Adult and Younger Cynomolgus Monkeys

2011 ◽  
Vol 100 (9) ◽  
pp. 3939-3950 ◽  
Author(s):  
Katsuaki Ito ◽  
Yasuo Uchida ◽  
Sumio Ohtsuki ◽  
Sanshiro Aizawa ◽  
Hirotaka Kawakami ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Cynomolgus Monkeys ◽  
Membrane Protein Expression ◽  
Blood Brain

scholarly journals Sex Hormones Regulate Rat Hepatic Monocarboxylate Transporter Expression and Membrane Trafficking

2017 ◽  
Vol 20 (1) ◽  
pp. 435 ◽  
Author(s):  
Jieyun Cao ◽  
Michael Ng ◽  
Melanie A Felmlee
Keyword(s):  
Sex Differences ◽  
Membrane Protein ◽  
Protein Expression ◽  
Estrous Cycle ◽  
Sex Hormones ◽  
Membrane Trafficking ◽  
Drug Disposition ◽  
Membrane Expression ◽  
Membrane Protein Expression ◽  
Estrous Cycle Stage

Purpose: Monocarboxylate transporters (MCTs) are involved in the transport of monocarboxylates such as ketone bodies, lactate, and pharmaceutical agents. CD147 functions as an ancillary protein for MCT1 and MCT4 for plasma membrane trafficking. Sex differences in MCT1 and MCT4 have been observed in muscle and reproductive tissues; however, there is a paucity of information on MCT sex differences in tissues involved in drug disposition. The objective of the present study was to quantify hepatic MCT1, MCT4 and CD147 mRNA, total cellular and membrane protein expression in males, over the estrous cycle in females and in ovariectomized (OVX) females. Method: Liver samples were collected from females at the four estrous cycle stages (proestrus, estrus, metestrus, diestrus), OVX females and male Sprague-Dawley rats (N = 3 – 5). Estrus cycle stage of females was determined by vaginal lavage. mRNA and protein (total and membrane) expression of MCT1, MCT4 and CD147 was evaluated by qPCR and western blot analysis. Results: MCT1 mRNA and membrane protein expression varied with estrous cycle stage, with OVX females having higher expression than males, indicating that female sex hormones may play a role in MCT1 regulation. MCT4 membrane expression varied with estrous cycle stage with expression significantly lower than males. MCT4 membrane expression in OVX females was also lower than males, suggesting that androgens play a role in membrane expression of MCT4. Males had higher membrane CD147 expression, whereas there was no difference in whole cell protein and mRNA levels suggesting that androgens are involved in regulating CD147 membrane localization. Conclusions: This study demonstrates hepatic expression and membrane localization of MCT1, MCT4 and CD147 are regulated by sex hormones. Sex differences in hepatic MCT expression may lead to altered drug disposition, so it is critical to elucidate the underlying mechanisms in the sex hormone-dependent regulation of MCT expression. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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