Discovery of behaviorally active semiochemicals in Aenasius bambawalei using a reverse chemical ecology approach

2021 ◽  
Author(s):  
Chong Xu ◽  
Fuxiang Yang ◽  
Shuanggang Duan ◽  
Dongzhen Li ◽  
Lei Li ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
Reverse Chemical Ecology

scholarly journals A reverse chemical ecology approach to explore wood natural durability

2020 ◽  
Vol 13 (5) ◽  
pp. 1673-1677 ◽  
Author(s):  
Thomas Perrot ◽  
Guillaume Salzet ◽  
Nadine Amusant ◽  
Jacques Beauchene ◽  
Philippe Gérardin ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
Natural Durability ◽  
Reverse Chemical Ecology

scholarly journals Reverse chemical ecology-based approach leading to the accidental discovery of repellents for Rhodnius prolixus, a vector of Chagas diseases refractory to DEET

2018 ◽  
Vol 103 ◽  
pp. 46-52 ◽  
Author(s):  
Thiago A. Franco ◽  
Pingxi Xu ◽  
Nathália F. Brito ◽  
Daniele S. Oliveira ◽  
Xiaolan Wen ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
Rhodnius Prolixus ◽  
Reverse Chemical Ecology ◽  
Accidental Discovery

Optimization of reverse chemical ecology method: false positive binding of Aenasius bambawalei odorant binding protein 1 caused by uncertain binding mechanism

2018 ◽  
Vol 27 (3) ◽  
pp. 305-318 ◽  
Author(s):  
Q.L. Li ◽  
S.C. Yi ◽  
D.Z. Li ◽  
X.P. Nie ◽  
S.Q. Li ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
False Positive ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Binding Mechanism ◽  
Odorant Binding ◽  
Reverse Chemical Ecology

scholarly journals Reverse chemical ecology at the service of conservation biology

2017 ◽  
Vol 114 (46) ◽  
pp. 12094-12096 ◽  
Author(s):  
Walter S. Leal
Keyword(s):  
Conservation Biology ◽  
Chemical Ecology ◽  
Reverse Chemical Ecology

scholarly journals Reverse chemical ecology in a moth: machine learning on odorant receptors identifies new behaviorally active agonists

2021 ◽  
Author(s):  
Gabriela Caballero-Vidal ◽  
Cédric Bouysset ◽  
Jérémy Gévar ◽  
Hayat Mbouzid ◽  
Céline Nara ◽  
...  
Keyword(s):  
Machine Learning ◽  
Chemical Ecology ◽  
Rational Design ◽  
Crop Protection ◽  
Odorant Receptors ◽  
Pest Insect ◽  
Crop Pest ◽  
Y Maze ◽  
Highly Sensitive ◽  
Reverse Chemical Ecology

AbstractThe concept of reverse chemical ecology (exploitation of molecular knowledge for chemical ecology) has recently emerged in conservation biology and human health. Here, we extend this concept to crop protection. Targeting odorant receptors from a crop pest insect, the noctuid moth Spodoptera littoralis, we demonstrate that reverse chemical ecology has the potential to accelerate the discovery of novel crop pest insect attractants and repellents. Using machine learning, we first predicted novel natural ligands for two odorant receptors, SlitOR24 and 25. Then, electrophysiological validation proved in silico predictions to be highly sensitive, as 93% and 67% of predicted agonists triggered a response in Drosophila olfactory neurons expressing SlitOR24 and SlitOR25, respectively, despite a lack of specificity. Last, when tested in Y-maze behavioral assays, the most active novel ligands of the receptors were attractive to caterpillars. This work provides a template for rational design of new eco-friendly semiochemicals to manage crop pest populations.

scholarly journals Reverse chemical ecology approach for the identification of an oviposition attractant for Culex quinquefasciatus

2018 ◽  
Vol 115 (4) ◽  
pp. 714-719 ◽  
Author(s):  
Young-Moo Choo ◽  
Pingxi Xu ◽  
Justin K. Hwang ◽  
Fangfang Zeng ◽  
Kaiming Tan ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
Culex Quinquefasciatus ◽  
Management Strategies ◽  
Practical Applications ◽  
Oviposition Attractant ◽  
Wide Range ◽  
Vector Management ◽  
Electroantennogram Recordings ◽  
Olfactory Proteins ◽  
Reverse Chemical Ecology

Pheromones and other semiochemicals play a crucial role in today’s integrated pest and vector management strategies. These semiochemicals are typically discovered by bioassay-guided approaches. Here, we applied a reverse chemical ecology approach; that is, we used olfactory proteins to lead us to putative semiochemicals. Specifically, we used 7 of the top 10 odorant receptors (ORs) most expressed in the antennae of the southern house mosquito, Culex quinquefasciatus, and which are yet to be deorphanized. We expressed these receptors in the Xenopus oocyte recording system and challenged them with a panel of 230 odorants, including physiologically and behaviorally active compounds. Six of the ORs were silent either because they are not functional or a key odorant was missing. CquiOR36, which showed the highest transcript levels of all OR genes in female antennae, was also silent to all odorants in the tested panel, but yielded robust responses when it was accidentally challenged with an old sample of nonanal in ethanol. After confirming that fresh samples were inactive and through a careful investigation of all possible “contaminants” in the old nonanal samples, we identified the active ligand as acetaldehyde. That acetaldehyde is activating CquiOR36 was further confirmed by electroantennogram recordings from antennae of fruit flies engineered to carry CquiOR36. Antennae of female mosquitoes also responded to acetaldehyde. Cage oviposition and dual-choice assays demonstrated that acetaldehyde is an oviposition attractant in a wide range of concentrations and thus of potential practical applications.

scholarly journals A reverse chemical ecology approach to explore wood natural durability

2019 ◽  
Author(s):  
Perrot Thomas ◽  
Salzet Guillaume ◽  
Amusant Nadine ◽  
Beauchene Jacques ◽  
Gérardin Philippe ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
Abiotic Factors ◽  
Antimicrobial Properties ◽  
White Rot ◽  
Glutathione Transferases ◽  
Natural Durability ◽  
Model Combining ◽  
Wood Extracts ◽  
Detoxification Systems ◽  
Reverse Chemical Ecology

SummaryThe natural durability of wood species, defined as their inherent resistance to wood-destroying agents is a complex phenomenon depending of many biotic and abiotic factors. Besides the presence of recalcitrant polymers, the presence of compounds with antimicrobial properties is known to be important to explain wood durability. Based on the advancement in our understanding of fungal detoxification systems, a reverse chemical ecology approach was proposed to explore wood natural durability. A set of six glutathione transferases from the white-rot Trametes versicolor was used as targets to test wood extracts from seventeen French Guiana neotropical species. Fluorescent thermal shift assays allowed to quantify interactions between fungal glutathione transferases and these extracts. From these data, a model combining this approach and wood density predicts significantly wood natural durability of the tested species previously estimated by long-term soil bed tests. Overall, our findings confirm that detoxification systems could be used to explore the chemical environment encountered by wood decaying fungi and then wood natural durability.

Reverse chemical ecology guides the screening for Grapholita molesta pheromone synergists

2021 ◽  
Author(s):  
Jiyuan Liu ◽  
Tong Zhou ◽  
Chaoxia Li ◽  
Ruichi Li ◽  
Xuan Ye ◽  
...  
Keyword(s):  
Chemical Ecology ◽  
Grapholita Molesta ◽  
Reverse Chemical Ecology
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