scholarly journals Diamide insecticide resistance in transgenic Drosophila and Sf9‐cells expressing a full‐length diamondback moth ryanodine receptor carrying an I4790M mutation

2021 ◽  
Author(s):  
Ewan Richardson ◽  
Rafael A Homem ◽  
Bartlomiej J Troczka ◽  
Christopher H George ◽  
Ulrich Ebbinghaus‐Kintscher ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Ryanodine Receptor ◽  
Diamondback Moth ◽  
Full Length ◽  
Sf9 Cells ◽  
Transgenic Drosophila ◽  
Diamide Insecticide

Ryanodine receptor mutations (G4946E and I4790K) differentially responsible for diamide insecticide resistance in diamondback moth, Plutella xylostella L.

2020 ◽  
Vol 118 ◽  
pp. 103308 ◽  
Author(s):  
Akiya Jouraku ◽  
Seigo Kuwazaki ◽  
Kazuhisa Miyamoto ◽  
Makoto Uchiyama ◽  
Tatsuki Kurokawa ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Ryanodine Receptor ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Diamide Insecticide

Origin, selection, and spread of diamide insecticide resistance allele in field populations of diamondback moth in east and southeast Asia

2020 ◽  
Vol 77 (1) ◽  
pp. 313-324
Author(s):  
Ryuji Uesugi ◽  
Akiya Jouraku ◽  
Suprada Sukonthabhirom na Pattalung ◽  
Norihide Hinomoto ◽  
Seigo Kuwazaki ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Insecticide Resistance ◽  
Diamondback Moth ◽  
Resistance Allele ◽  
East And Southeast Asia ◽  
Diamide Insecticide

Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae)

2017 ◽  
Vol 80 ◽  
pp. 11-20 ◽  
Author(s):  
Emmanouil Roditakis ◽  
Denise Steinbach ◽  
Gerald Moritz ◽  
Emmanouil Vasakis ◽  
Marianna Stavrakaki ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Ryanodine Receptor ◽  
Point Mutations ◽  
Tuta Absoluta ◽  
Tomato Leafminer ◽  
Diamide Insecticide

Identification of the ryanodine receptor mutation I4743M and its contribution to diamide insecticide resistance in Spodoptera exigua (Lepidoptera: Noctuidae)

2019 ◽  
Vol 27 (4) ◽  
pp. 791-800 ◽  
Author(s):  
Ya‐Yun Zuo ◽  
Huan‐Huan Ma ◽  
Wen‐Jie Lu ◽  
Xing‐Liang Wang ◽  
Shu‐Wen Wu ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Ryanodine Receptor ◽  
Spodoptera Exigua ◽  
Lepidoptera Noctuidae ◽  
Diamide Insecticide

Varying contributions of three ryanodine receptor point mutations to diamide insecticide resistance in Plutella xylostella

2021 ◽  
Author(s):  
Dong Jiang ◽  
Cheng Qian ◽  
Danhui Wang ◽  
Falong Wang ◽  
Shan Zhao ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Ryanodine Receptor ◽  
Plutella Xylostella ◽  
Point Mutations ◽  
Diamide Insecticide

scholarly journals Gut Microbiota Mediate Insecticide Resistance in the Diamondback Moth, Plutella xylostella (L.)

2018 ◽  
Vol 9 ◽  
Author(s):  
Xiaofeng Xia ◽  
Botong Sun ◽  
Geoff M. Gurr ◽  
Liette Vasseur ◽  
Minqian Xue ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Insecticide Resistance ◽  
Plutella Xylostella ◽  
Diamondback Moth

scholarly journals Role of the G-Protein-Coupled Receptor Signaling Pathway in Insecticide Resistance

2019 ◽  
Vol 20 (17) ◽  
pp. 4300 ◽  
Author(s):  
Ting Li ◽  
Nannan Liu
Keyword(s):  
Insecticide Resistance ◽  
Cell Line ◽  
Signaling Pathway ◽  
G Protein ◽  
Cell Lines ◽  
Sf9 Cells ◽  
Camp Production ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

The G-protein-coupled receptor (GPCR) regulated intracellular signaling pathway is known to be involved in the development of insecticide resistance in the mosquito, Culex quinquefasciatus. To elucidate the specific role of each effector in the GPCR regulating pathway, we initially expressed a GPCR, G-protein alpha subunit (Gαs), adenylate cyclase (AC), and protein kinase A (PKA) in insect Spodoptera frugiperda (Sf9) cells and investigated their regulation function on cyclic AMP (cAMP) production and PKA activity. GPCR, Gαs, and AC individually expressed Sf9 cells showed higher cAMP production as the expression of each effector increased. All the effector-expressed cell lines showed increased PKA activity however. Moreover, Sf9 cytochrome P450 gene expression and cell tolerance to permethrin were examined. The relative expression of CYP9A32gene in Sf9 cells tested was significantly increased in all effector-expressed cell lines compared to a control cell line; these effector-expressed cell lines also showed significantly higher tolerance to permethrin. Inhibitor treatments on each effector-expressed cell line revealed that Bupivacaine HCl and H89 2HCl robustly inhibited cAMP production and PKA activity, respectively, resulting in decreased tolerance to permethrin in all cell lines. The synergistic functions of Bupivacaine HCl and H89 2HCl with permethrin were further examined in Culex mosquito larvae, providing a valuable new information for mosquito control strategies.

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