Determination of glyoxylyl-peptide concentration using oxime chemistry and RP-HPLC analysis

2006 ◽  
Vol 12 (11) ◽  
pp. 734-738 ◽  
Author(s):  
Jean-Michel Garcia ◽  
Samia Far ◽  
Eric Diesis ◽  
Oleg Melnyk
Keyword(s):  
Hplc Analysis ◽  
Peptide Concentration ◽  
Rp Hplc

Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection

2015 ◽  
Vol 98 (4) ◽  
pp. 939-945 ◽  
Author(s):  
Işil Gazioğlu ◽  
Ufuk Kolak
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Aflatoxin B1 ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Immunoaffinity Column ◽  
Rp Hplc

Abstract Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol–water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.

scholarly journals HPLC Analysis of Kaempherol and Quercetin Derivatives Isolated by Different Extraction Techniques from Plant Matrix

2011 ◽  
Vol 94 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Krystyna Skalicka-Woźniak ◽  
Janusz Szypowski ◽  
Kazimierz Głowniak
Keyword(s):  
Hplc Analysis ◽  
Soxhlet Extraction ◽  
Array Detector ◽  
Extraction Techniques ◽  
Ultrasound Extraction ◽  
Quercetin Derivatives ◽  
Rp Hplc ◽  
Photodiode Array Detector ◽  
Photodiode Array

Abstract Soxhlet extraction, ultrasound extraction, and accelerated solvent extraction (ASE), followed by RP-HPLC with a photodiode array detector was used for the determination of flavonoids in fruits of Peucedanum alsaticum. Three compounds were identified: a kaempherol derivative (astragalin), and two quercetin derivatives (quercitrin and hiperoside). The highest extraction yields of the selected compounds were obtained by use of exhaustive ASE.

Quantitative determination of three pentacyclic triterpenes from fiveSwertiaL. species endemic to Western Ghats, India, using RP-HPLC analysis

2015 ◽  
Vol 29 (19) ◽  
pp. 1783-1788 ◽  
Author(s):  
Parthraj R. Kshirsagar ◽  
Sandeep R. Pai ◽  
Mansingraj S. Nimbalkar ◽  
Nikhil B. Gaikwad
Keyword(s):  
Quantitative Determination ◽  
Western Ghats ◽  
Hplc Analysis ◽  
Pentacyclic Triterpenes ◽  
Rp Hplc

Development and Validation of a RP-HPLC Method for Simultaneous Determination of Levofloxacin and Moxifloxacin in Pharmaceutical Dosage Forms

2014 ◽  
Vol 12 (2) ◽  
Author(s):  
Farjahan Nur ◽  
Asma Rahman ◽  
Md. Zakir Sultan ◽  
Md. Gias Uddin ◽  
Mohammad A Rashid
Keyword(s):  
Simultaneous Determination ◽  
Dosage Forms ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Rp Hplc ◽  
Development And Validation

RP-HPLC method development for simultaneous determination of the drugs Ramipril and Amlodipine

2012 ◽  
Vol 2 (2) ◽  
pp. 364-367 ◽  
Author(s):  
Saida Naik Dheeravath ◽  
◽  
Kasani Ramadevi ◽  
Zilla Saraswathi ◽  
Dheeravath Maniklal ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Method Development ◽  
Hplc Method ◽  
Rp Hplc ◽  
Hplc Method Development

RP-HPLC analysis of withanolides in the flowers, leaves, and roots ofWithania somnifera

2010 ◽  
Vol 22 (3) ◽  
pp. 473-480 ◽  
Author(s):  
N. W. Ali ◽  
S. Abouzid ◽  
A. Nasib ◽  
S. Khan ◽  
J. Qureshi ◽  
...  
Keyword(s):  
Hplc Analysis ◽  
Rp Hplc ◽  
Leaves And Roots
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