peptide concentration
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PROTEOMICS ◽  
2021 ◽  
pp. 2100129
Author(s):  
Peter R. Mosen ◽  
Robert Hardt ◽  
Dominic Winter

2021 ◽  
Vol 11 (2) ◽  
pp. 37-39
Author(s):  
Irina Ostroukhova ◽  
Olga Rudko ◽  
Artemii Tretiakov

Migraine is diagnosed in approximately 15% of the population in the developed countries. This disease affects not only patient’s well-being, but also economy and social sphere. Despite this significant impact, little is known on the genetic causes of migraine. Several migraine symptoms, including tiredness, yawning, drowsiness, and the desire to eat certain foods, give an idea of migraine connection to orexin system. This system includes orexines – peptides, predominantly synthesized in the lateral hypothalamic area and involved in wake and sleep cycle and many other neurological functions; and their two receptors HCRTR1 and HCRTR2. Here we summarize known data on orexin system polymorphisms and changes in peptide concentration in patients with migraine.


2020 ◽  
Vol 27 (5) ◽  
pp. 53-59
Author(s):  
D. A. Bahrij ◽  
O. L. Starzhynska ◽  
V. M. Zhebel

The aim – to determine the place of soluble ST2 (sST2) as a possible biomarker of remodeling and heart dysfunction in patients with essential hypertension of varying severity.Materials and methods. Examined 150 men with confirmed essential hypertension (EН) of varying severity, including 50 people with EН complicated by heart failure (HF) IIA stage according to the classification of UAHF (groups were representative by age, mean age 50.17±0.48 y.о.), 70 men in the control group (mean age 48.82±0.78 y.о.) The following inclusion criteria were used: age 40 to 60 years, male, verified diagnosis of EH. The structure and function of the heart were studied using echocardiography with Doppler. The serum level of sST2 was determined by immuno assay.Results and discussion. The serum level of sST2 in patients of the control group was 22.14±0.86 ng/ml. It was found, that the peptide concentration in plasma of male patients without cardiovascular disease does not significantly correlate with physical or age parameters, renal function, the condition of systemic or intracardiac hemodynamics. It was found, that for patients with EH the serum level of sST2 was significantly higher than in the control group (p<0.05). The formation of left ventricular hypertrophy (LVH) leads to a significant increase of the peptide concentration in plasma in patients with EH. However, in patients with different types of LVH – concentric and eccentric – the indicator does not differ significantly (26.87±1.04 ng/ml vs. 29.15±1.15 ng/ml, p≥0.05). It was determined, that in case of confirmed diastolic dysfunction (DD) without LVH, the level of the peptide does not increase significantly. And only in patients with a combination of DD and LVH the serum level of sST2 is significantly higher (27.64±1.17 ng/ml, p≥0.05). Also in patients with EH and left ventricular ejection fraction (LV EF) less than 40 %, the serum level of peptide is significantly higher, than in patients with preserved LV EF (25.65±1.14 ng/ml vs. 29.07±1.22 ng/ml, p≥0.01).Conclusions. Thus, in male patients with EH of varying severity, the serum level of sST2 is significantly higher than in people without cardiovascular disease. Peptide concentration in plasma in both cases is not related to age or physical parameters. In patients with uncomplicated EH, significantly higher serum level of sST2 ​​is associated with the development of LVH, regardless of its type, and impaired diastolic function of the heart. In case of HF on the background of EH, the serum level of sST2 also is significantly higher, especially in case of the formation of systolic heart dysfunction with LV FE < 40 %.


2020 ◽  
Vol 24 (10) ◽  
pp. 1827-1834
Author(s):  
P.O. Oyedoh ◽  
C.E. Oshoma ◽  
M.J. Ikenebumeh

Lactic acid bacteria elective habitat is food matrix, where they release encrypted metabolites from several parent proteins as a result of their proteolytic activity in the matrix. These metabolites when decrypted confer different bioactive activity thus improving public health. This study was aimed at producing peptide containing metabolites with antibacterial efficacy from defatted cowpea and peanut using Lactobacillus spp. Lactobacillus spp. isolated from spoilt yoghurt were identified using culture dependent and independent method. The isolates were screened for proteolytic ability on skimmed milk agar. The selected isolate with highest proteolytic activity was used for metabolites production through fermentation of defatted cowpea and peanut at 37 oC for 72 h. Parameters analyzed during fermentation were pH, Lactobacillus count, protease activity, peptide concentration and antibacterial activity. The crude peptides produced were assayed for antibacterial activity against bacteria isolated from spoilt meat. From the identified Lactobacillus spp., L. plantarum CAU4347 had the highest proteolytic activity with clear zone of 24.50 ± 0.707 mm. During fermentation the highest and lowest Lactobacillus counts were from cowpea and peanut media with values 294.2 ± 0.21 ×107 and 0.60 ± 0.4 ×107 cfu/ml respectively. Cowpea medium inoculated with L. plantarum CAU4347, had the highest peptide concentration of 79.92 ± 0.01 µg/ml. Consequently, peanut medium showed higher antibacterial activity of 18 mm against Escherichia coli. This result finding suggests that encrypted peptide metabolites from cowpea and peanut flour can confer antibacterial activity against meat spoilage bacteria thus could be utilized as a potential bio-preservative. Keywords: Metabolites, Cowpea, Peanut, Bioactive, Fermentation, Lactobacillus plantarum


Membranes ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 90 ◽  
Author(s):  
Rémi Przybylski ◽  
Laurent Bazinet ◽  
Loubna Firdaous ◽  
Mostafa Kouach ◽  
Jean-François Goossens ◽  
...  

The fractionation of bioactive peptides from hydrolysate is a main challenge to produce efficient alternative for synthetic additives. In this work, electrodialysis with ultrafiltration membrane (EDUF) was proposed to increase the purity of one antimicrobial peptide from slaughterhouse by-product hydrolysate. This targeted-peptide, α137–141 (653 Da, TSKYR), inhibits a large spectrum of microbial growths and delays meat rancidity; therefore, if concentrated, it could be used as food antimicrobial. In this context, three pH values were investigated during EDUF treatment to increase the α137–141 purity: 4.7, 6.5, and 9. pH 9 showed the highest purity increase—75-fold compared to the initial hydrolysate. Although the whole hydrolysate contains more than 100 peptides, only six peptides were recovered at a significant concentration. In this fraction, the α137–141 peptide represented more than 50% of the recovered total peptide concentration. The EDUF α137–141-enriched fraction obtained in this optimized condition would be a promising natural preservative to substitute synthetic additives used to protect food.


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