Regulation of insulin action by an extract of Artemisia dracunculus L. in primary human skeletal muscle culture: A proteomics approach

2010 ◽  
Vol 24 (9) ◽  
pp. 1278-1284 ◽  
Author(s):  
Indu Kheterpal ◽  
Liana Coleman ◽  
Ginger Ku ◽  
Zhong Q. Wang ◽  
David Ribnicky ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Action ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture ◽  
Proteomics Approach

scholarly journals Bioactives of Artemisia dracunculus L enhance cellular insulin signaling in primary human skeletal muscle culture

Metabolism ◽  
2008 ◽  
Vol 57 ◽  
pp. S58-S64 ◽  
Author(s):  
Zhong Q. Wang ◽  
David Ribnicky ◽  
Xian H. Zhang ◽  
Ilya Raskin ◽  
Yongmei Yu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture

scholarly journals Bioactives of Artemisia dracunculus L. ameliorates insulin sensitivity by attenuating inflammatory signalling in human skeletal muscle culture (LB333)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Bolormaa Vandanmagsar ◽  
Kimberly Haynie ◽  
Shawna Wicks ◽  
Estrellita Bermudez ◽  
Tamra Mendoza ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Insulin Sensitivity ◽  
Human Skeletal Muscle ◽  
Artemisia Dracunculus ◽  
Muscle Culture ◽  
Inflammatory Signalling

Does impaired mitochondrial function affect insulin signaling and action in cultured human skeletal muscle cells?

2008 ◽  
Vol 294 (1) ◽  
pp. E97-E102 ◽  
Author(s):  
Audrey E. Brown ◽  
Matthias Elstner ◽  
Stephen J. Yeaman ◽  
Douglass M. Turnbull ◽  
Mark Walker
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Insulin Signaling ◽  
Mitochondrial Respiration ◽  
Respiratory Function ◽  
Insulin Action ◽  
Human Skeletal Muscle ◽  
Human Skeletal Muscle Cells ◽  
Basal Glucose Uptake

Insulin-resistant type 2 diabetic patients have been reported to have impaired skeletal muscle mitochondrial respiratory function. A key question is whether decreased mitochondrial respiration contributes directly to the decreased insulin action. To address this, a model of impaired cellular respiratory function was established by incubating human skeletal muscle cell cultures with the mitochondrial inhibitor sodium azide and examining the effects on insulin action. Incubation of human skeletal muscle cells with 50 and 75 μM azide resulted in 48 ± 3% and 56 ± 1% decreases, respectively, in respiration compared with untreated cells mimicking the level of impairment seen in type 2 diabetes. Under conditions of decreased respiratory chain function, insulin-independent (basal) glucose uptake was significantly increased. Basal glucose uptake was 325 ± 39 pmol/min/mg (mean ± SE) in untreated cells. This increased to 669 ± 69 and 823 ± 83 pmol/min/mg in cells treated with 50 and 75 μM azide, respectively (vs. untreated, both P < 0.0001). Azide treatment was also accompanied by an increase in basal glycogen synthesis and phosphorylation of AMP-activated protein kinase. However, there was no decrease in glucose uptake following insulin exposure, and insulin-stimulated phosphorylation of Akt was normal under these conditions. GLUT1 mRNA expression remained unchanged, whereas GLUT4 mRNA expression increased following azide treatment. In conclusion, under conditions of impaired mitochondrial respiration there was no evidence of impaired insulin signaling or glucose uptake following insulin exposure in this model system.

Susceptibility of human skeletal muscle culture to influenza virus infection

1978 ◽  
Vol 35 (1) ◽  
pp. 43-57 ◽  
Author(s):  
C.L. Armstrong ◽  
A.F. Miranda ◽  
K.C. Hsu ◽  
E.T. Gamboa
Keyword(s):  
Skeletal Muscle ◽  
Influenza Virus ◽  
Virus Infection ◽  
Human Skeletal Muscle ◽  
Influenza Virus Infection ◽  
Muscle Culture

Effect of exercise on insulin action in human skeletal muscle

1989 ◽  
Vol 66 (2) ◽  
pp. 876-885 ◽  
Author(s):  
E. A. Richter ◽  
K. J. Mikines ◽  
H. Galbo ◽  
B. Kiens
Keyword(s):  
Skeletal Muscle ◽  
Glucose Uptake ◽  
Insulin Action ◽  
Human Skeletal Muscle ◽  
Vastus Lateralis ◽  
Femoral Vein ◽  
Glycogen Synthesis ◽  
Vastus Lateralis Muscle ◽  
Glycogen Concentration ◽  
Local Contraction

The effect of 1 h of dynamic one-legged exercise on insulin action in human muscle was studied in 6 healthy young men. Four hours after one-legged knee extensions, a three-step sequential euglycemic hyperinsulinemic clamp combined with arterial and bilateral femoral vein catheterization was performed. Increased insulin action on glucose uptake was found in the exercised compared with the rested thigh at mean plasma insulin concentrations of 23, 40, and 410 microU/ml. Furthermore, prior contractions directed glucose uptake toward glycogen synthesis and increased insulin effects on thigh O2 consumption and at some insulin concentrations on potassium exchange. In contrast, no change in insulin effects on limb exchange of free fatty acids, glycerol, alanine or tyrosine were found after exercise. Glycogen concentration in rested vastus lateralis muscle did not increase measurably during the clamp even though indirect estimates indicated net glycogen synthesis. In contrast, in exercised muscle estimated and biopsy-verified increases in muscle glycogen concentration agreed. Local contraction-induced increases in insulin sensitivity and responsiveness play an important role in postexercise recovery of human skeletal muscle.

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