In some species, stable-isotope techniques can provide insights into dietary regimens where there are temporal shifts in trophic level or feeding frequency. We determined stable carbon (δ13C) and nitrogen (δ15N) isotope values for plasma and milk proteins and δ13C values for milk lipids from female polar bears (Ursus maritimus) and cubs to (i) ascertain whether cubs are at a higher trophic level than their mothers as a result of nursing and whether we can determine when weaning occurs, and (ii) determine the impact of seasonal fasting on δ13C and δ15N values. The plasma δ13C values for mothers and cubs were similar to milk-protein δ13C values and were significantly enriched in 13C compared with those for milk lipid. Plasma from cubs of the year (COYs) in spring, when milk was their only diet, was isotopically enriched in 15N by 1.0 over that of their mothers (δ15N = 21.5 ± 0.8 (mean ± SD) for cubs and 20.5 ± 0.5 for mothers) and depleted in 13C by 0.8 (δ13C = 19.6 ± 0.5 for cubs and 18.8 ± 0.8 for mothers). For bears who fasted between summer and fall (34 months), plasma became depleted in 13C by 0.5 and in 15N by 1. Plasma from females, who had fasted from summer to spring (78 months) and given birth to cubs, became enriched in 13C by 0.7 and in 15N by 2. By using stable-isotope analyses we were able to show that (i) young cubs were at a higher trophic level than their mother when milk was their only food source, and (ii) seasonal fasting influenced δ13C and δ15N values. However, we were not able to use stable-isotope analyses to determine the exact time of weaning.
Carbon and nitrogen sources for the bivalve Corbicula japonica in Natori River estimated from stable isotope analyses
