This manual contains methods of chemical analysis of over 100 substances in biological fluids and should, therefore, provide a complete ready-reference source for clinical chemistry laboratories. The range of methods of chemical analysis which are given, extends from routine analysis, such as for bicarbonate, chloride, glucose, etc., to some rather more complicated and specialized techniques, such as for catecholamines, 17-hydroxycorticoids, mucopolysaccharides, etc. There is hardly a test which will be performed in the routine hospital laboratory which is not covered by this manual.