High-performance thin-layer chromatography: An economical alternative for the quality control of medicinal plants and derived products

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

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Reproducible reversed-phase high-performance thin-layer chromatography-based quality-control method for the endangered medicinal plantPicrorhiza kurroaRoyle ex Benth.

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/1006.2015.28.3.11 ◽

2015 ◽

Vol 28 (3) ◽

pp. 256-261 ◽

Cited By ~ 2

Author(s):

Dinesh Kumar ◽

Rakesh Kumar ◽

Bikram Singh ◽

Paramvir Singh Ahuja

Keyword(s):

Quality Control ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Control Method ◽

Reversed Phase ◽

Quality Control Method ◽

Layer Chromatography

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Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

Biomolecules ◽

10.3390/biom9110731 ◽

2019 ◽

Vol 9 (11) ◽

pp. 731 ◽

Cited By ~ 5

Author(s):

Juszczak ◽

Zovko-Končić ◽

Tomczyk

Keyword(s):

Liquid Chromatography ◽

Medicinal Plants ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Analytical Methods ◽

High Performance ◽

Biological Effects ◽

Immunomodulatory Activity ◽

Layer Chromatography

Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quantitative determination of luteolin and its C- and O-derivatives including orientin, isoorientin, luteolin 7-O-glucoside and others. It provides a systematic overview of chromatographic analytical techniques including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), liquid chromatography (LC), high performance liquid chromatography (HPLC), gas chromatography (GC) and counter-current chromatography (CCC), as well as the conditions used in the determination of luteolin and its derivatives in plant material.

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