Reproducible reversed-phase high-performance thin-layer chromatography-based quality-control method for the endangered medicinal plantPicrorhiza kurroaRoyle ex Benth.

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

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Front Cover: High-performance thin-layer chromatography: An economical alternative for the quality control of medicinal plants and derived products

Separation Science Plus ◽

10.1002/sscp.201870007 ◽

2018 ◽

Vol 1 (2) ◽

pp. NA-NA

Author(s):

Dinesh Kumar ◽

Upendra Sharma

Keyword(s):

Quality Control ◽

Medicinal Plants ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Front Cover ◽

Layer Chromatography ◽

Economical Alternative

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Preparative reversed-phase high-performance thin-layer chromatography for analysis of anthocyanins

JPC - Journal of Planar Chromatography - Modern TLC ◽

10.1556/jpc.19.2006.6.10 ◽

2006 ◽

Vol 19 (112) ◽

pp. 463-466 ◽

Cited By ~ 2

Author(s):

Agnieszka Skalska ◽

Anna Matysik ◽

Marek Gerkowicz ◽

Magdalena Wójciak-Kosior

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Reversed Phase ◽

Layer Chromatography

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Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

Scientific Reports ◽

10.1038/s41598-019-53468-9 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Beata Polak ◽

Adam Traczuk ◽

Sylwia Misztal

Keyword(s):

Amino Acid ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Amino Acid Derivatives ◽

Solute Retention ◽

Micellar Systems ◽

Layer Chromatography

AbstractThe problems with separation of amino acid mixtures in reversed-phase mode are the result of their hydrophilic nature. The derivatisation of the amino group of mentioned above solutes leads to their solution. For this purpose, 9-fluorenylmethoxycarbonyl chloroformate (f-moc-Cl) as the derivatisation reagent is often used. In our study, the separation of some f-moc- amino acid derivatives (alanine, phenylalanine, leucine, methionine, proline and tryptophan) with the use of micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) is investigated. The effect of surfactant concentration, its type (anionic, cationic and non-ionic) and mobile phase buffer pH on the discussed above solute migration distances are presented. Our work reveals that the increase of sodium dodecylsulphate concentration in the mobile phase has a different effect on solute retention in HPTLC and PPEC. Moreover, it also affects the order of solutes in both techniques. In PPEC, in contrast to the HPTLC technique, the mobile phase pH affects solute retention. The type of surfactant in the mobile phase also impacts solute retention and migration distances. A mobile phase containing SDS improves system efficiency in both techniques. Herein, such an effect is presented for the first time.

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