scholarly journals Bone morphogenetic protein signaling regulates Id1-mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module

Stem Cells ◽  
2020 ◽  
Author(s):  
Gaoqun Zhang ◽  
Marco Ferg ◽  
Luisa Lübke ◽  
Masanari Takamiya ◽  
Tanja Beil ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Neural Stem Cell ◽  
Protein Signaling ◽  
Adult Zebrafish ◽  
Bone Morphogenetic Protein Signaling ◽  
Zebrafish Brain ◽  
Morphogenetic Protein ◽  
Stem Cell Quiescence ◽  
Cell Quiescence

scholarly journals Bone morphogenetic protein signaling regulates Id1 mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module

2019 ◽  
Author(s):  
Gaoqun Zhang ◽  
Marco Ferg ◽  
Luisa Lübke ◽  
Masanari Takamiya ◽  
Tanja Beil ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bmp Signaling ◽  
Transgenic Zebrafish ◽  
Deletion Mapping ◽  
Protein Signaling ◽  
Adult Zebrafish ◽  
Specific Expression ◽  
Radial Glial Cells ◽  
Morphogenetic Protein ◽  
Stem Cell Quiescence

AbstractIn the telencephalon of adult zebrafish, the inhibitor of DNA binding 1 (id1) gene is expressed in radial glial cells (RGCs), behaving as neural stem cells (NSCs), during constitutive and regenerative neurogenesis. Id1 controls the balance between resting and proliferating states of RGCs by promoting quiescence. Here, we identified a phylogenetically conserved cis-regulatory module (CRM) mediating the specific expression of id1 in RGCs. Systematic deletion mapping and mutation of conserved transcription factor binding sites in stable transgenic zebrafish lines reveal that this CRM operates via conserved smad1/5 and 4 binding motifs (SBMs) under both homeostatic and regenerative conditions. Transcriptome analysis of injured and uninjured telencephala as well as pharmacological inhibition experiments identify a crucial role of bone morphogenetic protein (BMP) signaling for the function of the CRM. Our data highlight that BMP signals control id1 expression and thus NSC proliferation during constitutive and induced neurogenesis.

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