scholarly journals Spatiotemporal extracellular matrix modeling for in situ cell niche studies

Stem Cells ◽  
2021 ◽  
Author(s):  
Kim Olesen ◽  
Sergey Rodin ◽  
Wing Cheung Mak ◽  
Ulrika Felldin ◽  
Cecilia Österholm ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Modeling ◽  
Cell Niche

scholarly journals Publisher Correction: Quantitative and organisational changes in mature extracellular matrix revealed through high-content imaging of total protein fluorescently stained in situ

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Gill Holdsworth ◽  
Hélène Bon ◽  
Marianne Bergin ◽  
Omar Qureshi ◽  
Ross Paveley ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Total Protein ◽  
High Content Imaging

scholarly journals Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering

2017 ◽  
Vol 17 (3) ◽  
pp. 435-444 ◽  
Author(s):  
Rebecca A. Wachs ◽  
Ella N. Hoogenboezem ◽  
Hammad I. Huda ◽  
Shangjing Xin ◽  
Stacy L. Porvasnik ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Tissue ◽  
In Situ Gelling

scholarly journals Quantitative and organisational changes in mature extracellular matrix revealed through high-content imaging of total protein fluorescently stained in situ

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Gill Holdsworth ◽  
Hélène Bon ◽  
Marianne Bergin ◽  
Omar Qureshi ◽  
Ross Paveley ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Total Protein ◽  
High Content Imaging

scholarly journals Extracellular matrix and the neural stem cell niche

2011 ◽  
Vol 71 (11) ◽  
pp. 1006-1017 ◽  
Author(s):  
Ilias Kazanis ◽  
Charles ffrench-Constant
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Neural Stem Cell ◽  
Stem Cell Niche ◽  
Neural Stem Cell Niche ◽  
Cell Niche

In situ Peptide Self-assembly on Ionic Nanochannel for Dynamic Monitoring of MMPs in Extracellular Matrix

2021 ◽  
pp. 113671
Author(s):  
Lin Wang ◽  
Hao Li ◽  
Liu Shi ◽  
Li Li ◽  
Fengjie Jia ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Self Assembly ◽  
Dynamic Monitoring

Tenascin in rat lung development: in situ localization and cellular sources

1995 ◽  
Vol 269 (4) ◽  
pp. L482-L491 ◽  
Author(s):  
Y. Zhao ◽  
S. L. Young
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Epithelial Cells ◽  
Lung Tissue ◽  
Lung Development ◽  
Alveolar Epithelial Cells ◽  
Lung Fibroblasts ◽  
Rat Lung ◽  
Alveolar Epithelial

Tenascin (TN) is a hexameric extracellular matrix glycoprotein that may play an important role during lung development. TN protein is temporally and spatially restricted during lung organogenesis. The temporo-spatial and cellular expression of TN mRNA in lung remains unclear. Localization of message expression of TN in rat lung tissue was first investigated by using in situ hybridization performed with an antisense RNA probe. TN mRNA was present primarily within the mesenchyme of day 16 gestational age fetal rat lung tissue, whereas immunoreactive TN protein was found along the basement membrane. In postnatal day 3 rat lung tissue, TN mRNA was detected along alveolar septal walls and was concentrated at secondary septal tips. Expression of TN message was consistent with localization of immunoreactive TN protein. Accumulation of TN mRNA in alveolar septal tips suggests that mesenchyme may be the major source of TN mRNA. To investigate the cellular source of TN in rat lung, we studied the expression of TN in cultured rat lung fibroblasts, endothelial cells, and alveolar epithelial cells. Two TN isoforms having molecular mass of 230 and 180 kDa were in conditioned medium and in cellular extracts of lung fibroblasts and endothelial cells. TN was secreted and deposited in the extracellular matrix closely associated with the surface of lung fibroblasts and endothelial cells. Lung alveolar epithelial cells showed undetectable or barely detectable amounts of TN. These studies demonstrated that TN isoforms are expressed not only by lung fibroblasts but also by lung endothelial cells. The unique spatial localization of TN mRNA during lung development and expression of TN by different lung cell types suggested TN may be involved in matrix organization and cell-cell interactions during lung development.

In Situ Drug Delivery to Breast Cancer-Associated Extracellular Matrix

2018 ◽  
Vol 13 (10) ◽  
pp. 2825-2840 ◽  
Author(s):  
Jodie M. Fleming ◽  
Susan T. Yeyeodu ◽  
Ashley McLaughlin ◽  
Darren Schuman ◽  
Darlene K. Taylor
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Extracellular Matrix

Abstract A22: In situ image-based monitoring of extracellular matrix mechanics in 3D pancreatic tumor-stroma co-culture models to inform therapeutic development

2016 ◽  
Author(s):  
Dustin P. Jones ◽  
William Hanna ◽  
Gwendolyn M. Cramer ◽  
Hamid El-Hamidi ◽  
Jonathan P. Celli
Keyword(s):  
Extracellular Matrix ◽  
Pancreatic Tumor ◽  
Tumor Stroma ◽  
Matrix Mechanics ◽  
Therapeutic Development ◽  
Culture Models

In Situ Hierarchical Strain Transfer From the Articular Cartilage Surface to the Chondrocyte Nucleus

2012 ◽  
Author(s):  
Jonathan T. Henderson ◽  
Corey P. Neu
Keyword(s):  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Cartilage Surface ◽  
Strain Transfer ◽  
Nuclear Mechanics ◽  
Articular Cartilage Surface ◽  
Matrix Interactions ◽  
Wear And Tear ◽  
Extracellular Matrix Interactions

Osteoarthritis (OA) is a disabling disease, commonly thought of as the “wear and tear” of articular cartilage, afflicting 27 million Americans [1]. Multiple (e.g. biomechanical and biochemical) factors [2] contribute to maintenance of healthy joints through chondrocyte and extracellular matrix interactions. Interestingly, volumetric contractions of nuclei exhibit a zonal dependence [3], suggesting that nuclear mechanics may play a key role in the maintenance of healthy tissue by mechanically-mediated pathways.

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