scholarly journals A Nonhuman Primate Model for Urinary Bladder Regeneration Using Autologous Sources of Bone Marrow-Derived Mesenchymal Stem Cells

Stem Cells ◽  
2011 ◽  
Vol 29 (2) ◽  
pp. 241-250 ◽  
Author(s):  
Arun K. Sharma ◽  
Matthew I. Bury ◽  
Andrew J. Marks ◽  
Natalie J. Fuller ◽  
Jay W. Meisner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Urinary Bladder ◽  
Nonhuman Primate ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Bladder Regeneration

scholarly journals Repair of Infarcted Myocardium Mediated by Transplanted Bone Marrow-Derived CD34+Stem Cells in a Nonhuman Primate Model

Stem Cells ◽  
2005 ◽  
Vol 23 (3) ◽  
pp. 355-364 ◽  
Author(s):  
Toru Yoshioka ◽  
Naohide Ageyama ◽  
Hiroaki Shibata ◽  
Takanori Yasu ◽  
Yoshio Misawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Nonhuman Primate ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Infarcted Myocardium

scholarly journals A Nonhuman Primate Model of Lung Regeneration: Detergent-Mediated Decellularization and Initial In Vitro Recellularization with Mesenchymal Stem Cells

2012 ◽  
Vol 18 (23-24) ◽  
pp. 2437-2452 ◽  
Author(s):  
Ryan W. Bonvillain ◽  
Svitlana Danchuk ◽  
Deborah E. Sullivan ◽  
Aline M. Betancourt ◽  
Julie A. Semon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nonhuman Primate ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Lung Regeneration

Maternal High-Fat Diet Persistently Alters Bone Marrow Immune Cell Proportions and Function in a Nonhuman Primate Model

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 222-OR
Author(s):  
MICHAEL J. NASH ◽  
TAYLOR K. SODERBORG ◽  
RACHEL C. JANSSEN ◽  
ERIC M. PIETRAS ◽  
JACOB E. FRIEDMAN
Keyword(s):  
Bone Marrow ◽  
Nonhuman Primate ◽  
High Fat Diet ◽  
Immune Cell ◽  
High Fat ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
And Function

C53 HAIR FOLLICLE STEM CELLS AND BONE MARROW MESENCHYMAL STEM CELLS IN BLADDER REGENERATION

2010 ◽  
Vol 9 (6) ◽  
pp. 632-633
Author(s):  
R. Joachimiak ◽  
A. Bajek ◽  
Z. Cieslak ◽  
T. Drewa ◽  
M. Gagat ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Marrow Mesenchymal Stem Cells ◽  
Hair Follicle Stem Cells ◽  
Bladder Regeneration ◽  
Follicle Stem Cells

Use of Allogeneic Bone Marrow Labeled with Neomycin Resistance Gene to Examine Bone Marrow-Derived Chimerism in Experimental Organ Transplantation

1997 ◽  
Vol 6 (4) ◽  
pp. 369-376 ◽  
Author(s):  
J. P. Smith ◽  
J. Kasten-Jolly ◽  
L. Rebellato ◽  
Carl E. Haisch ◽  
Judith M. Thomas
Keyword(s):  
Bone Marrow ◽  
Resistance Gene ◽  
Nonhuman Primate ◽  
Antithymocyte Globulin ◽  
Molecular Techniques ◽  
Allogeneic Bone ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Neomycin Resistance Gene ◽  
Neomycin Resistance

Posttransplant infusion of viable donor bone marrow cells (DBMC) has been shown in our previous studies to promote acceptance of incompatible kidney allografts in rhesus monkeys after treatment with polyclonal antithymocyte globulin to deplete peripheral T-lymphocytes. In this nonhuman primate model, the infusion of the DBMC is requisite for the induction of functional graft tolerance and specific MLR and CTLp unresponsiveness, although the relevant role and fate of bone marrow-derived chimeric cells is uncertain. Standard immunological and molecular techniques applied to this monkey model are unable to differentiate between chimeric cells derived from the infused DBMC and those derived from allograft-borne passenger leukocyte emigrants. To distinguish chimerism due to infused DBMC, we transduced DBMC with a functional neomycin resistance gene (Neor) using the retroviral vector pHSG-Neo. Neor-Mransduced BMC were infused into recipients approximately 2 wk after kidney transplantation and treatment with rabbit antithymocyte globulin. No maintenance immunosuppressive drugs were given. Genomic DNA isolated from peripheral blood leukocytes was used to monitor the presence of Neor-positive cells. Tissue samples obtained at necropsy also were assessed for Neor-positive chimeric cells. The presence of DBMC-derived chimerism was assessed by polymerase chain reaction using Neor sequence-specific primers (PCR-SSP). Chimerism was detectable in recipient tissues at various times for up to 6 mo after DBMC infusion. These studies using gene transduction methodology indicate that a stable genetic marker can provide capability to examine DBMC-derived chimerism for prolonged periods in a nonhuman primate model. This approach should facilitate future studies in preclinical models to study the role and type of chimeric cell lineages in relation to functional allograft tolerance.

Efficient Transduction of Common Marmoset (Callithrix jacchus) Hematopoietic and Embryonic Stem Cells Using Foamyvirus Vectors.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5530-5530
Author(s):  
Peter A. Horn ◽  
Melanie Wurm ◽  
Ryo Kurita ◽  
Tomoko Yokoo ◽  
Rainer Blasczyk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Transfer ◽  
Nonhuman Primate ◽  
Embryonic Stem ◽  
Common Marmoset ◽  
Transduction Efficiency ◽  
Nonhuman Primate Model ◽  
Primate Model ◽  
Efficient Gene

Abstract Preclinical animal models are important for evaluating the safety and therapeutic efficacy of new therapeutic modalities such as gene therapy. From the different large animal models, nonhuman primate models have emerged over the last decades as highly desirable experimental systems from both a pathophysiologic and pharmacokinetic viewpoint and the study of nonhuman primates has provided important information on the efficacy and safety of gene therapy systems in vivo prior to human trials. The common marmoset (Callithrix jacchus) has the advantage that it is a small, and thus relatively inexpensive nonhuman primate model. Currently, very little data on the transduction efficiency of foamyviral vectors for gene transfer into marmoset stem cells exists. We therefore performed a direct comparison using identically designed gammaretroviral, lentiviral and foamyviral vector constructs expressing the enhanced green fluorescent protein (EGFP) from the spleen focus forming virus (SFFV) promoter pseudotyped with either the modified human foamy virus (HFV) envelope EM140 or the G-protein of vesicular stomatitis virus (VSV-G) for the transduction of common marmoset embryonic stem cells (CMES) as well as marmoset CD34+ hematopoietic progenitor cells. Virus stocks of these vectors were prepared by polyethyleneimine-mediated transfection of 293T cells and concentrated approximately 10-fold by centrifugation for 4 hours at 10.000 g at 4°C. Three different target cell populations were transduced: common marmoset embryonic stem cells (CMES) or cryopreserved CD34-enriched cells from bone marrow of a common marmoset either after a two-day prestimulation in the presence of IL-6, FLT3L, cSCF and TPO at a concentration of 100 ng/mL each, or after overnight incubation with 100 ng/mL SCF only. Equal numbers of cells were exposed to the four different vector preparations for 14 hours in 12-well dishes coated with CH-296. The read-out was based on fluorescence microscopy of colonies plated in methyl cellulose as well as flow cytometry (FACS). Foamyviral vectors with the foamyviral envelope were the most efficient gene transfer tool for marmoset hematopoietic CD34-positive cells with stable transduction rates of over 80% as assessed by flow cytometry at both 2 or 7 days after the end of transduction and on average 88% transduction efficiency into colony forming cells (CFU-C). Transduction of CFU-C with all other vector preparations was below 60%. In CMES, initial gene transfer rates of over 80% were achieved with the VSV-G pseudotype lentiviral vector, however, expression decreased to 13% after 7 days. In contrast, the foamyviral vector pseudotyped with the foamyviral envelope decreased only from 49% to 24% after 7 days. In conclusion, we achieved stable viral gene transfer and expression in CMES cells as well as highly efficient gene transfer into common marmoset hematopoietic CD34 positive cells using foamyviral vectors. These results suggest that foamyviral vectors may be highly feasible vectors for stem cell gene transfer and thus set the stage for a more detailed analysis of this vector system in transplantation studies in this nonhuman primate model.

scholarly journals The Role of Genetically Modified Mesenchymal Stem Cells in Urinary Bladder Regeneration

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138643 ◽  
Author(s):  
Devon C. Snow-Lisy ◽  
Edward C. Diaz ◽  
Matthew I. Bury ◽  
Natalie J. Fuller ◽  
Jessica H. Hannick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Urinary Bladder ◽  
Genetically Modified ◽  
Bladder Regeneration
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