As wind power in China is developing more and more rapidly, the characteristics of wind power output such as randomness and volatility have brought great pressure to the system peak load regulation. On the basis of defining negative peak regulation ability, this paper gives out the calculation formula of negative peak regulation ability including wind power and the main factors of influencing the negative peak regulation ability are calculated. Aimed at the regional power grid in the target year 2014, this paper makes some analytical prediction on such main factors and calculates the negative peak regulation ability, and the amount of the acceptance of wind power bound by it, and makes some sensitivity analysis of the negative peak regulation ability and the amount of the acceptance of wind power.