The advances in nerve tissue engineering: From fabrication of nerve conduit to
            in vivo
            nerve regeneration assays

Defected peripheral nerve regeneration is still a challenge in clinical treatment. Conductive polymers show great potential in nerve tissue engineering because of their electrical property based on bioelectricity in vivo. In this study, conductive composite nerve conduit was synthesized with tetra-aniline and poly-dl-lactic acid. Their properties and the differentiation of rat pheochromocytoma 12 (PC12) cells in vitro stimulated with 200 mV for 1 h were investigated. Different amounts of tetra-aniline (0%, 5%, 10%, and 15%) were used to synthesize the conduits with different conductivities (0, 0.00625, 0.01, and 0.025 s/m, respectively), tensile strengths (2.45, 3.40, 4.45, and 5.50 MPa, respectively), and contact angles (80°, 78.5°, 62.5°, and 61.5°, respectively). The percentage of neurite-bearing cells and the median neurite length increased with an obvious raise of the content of tetra-aniline. In addition, the conduit with subcutaneous implantable experiments in vivo showed less inflammatory response. These promising results illustrated that this poly-dl-lactic acid/tetra-aniline conductive composite conduit had potential for nerve tissue engineering.

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Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration

Engineering in Life Sciences ◽

10.1002/elsc.201400151 ◽

2015 ◽

Vol 15 (6) ◽

pp. 612-621 ◽

Cited By ~ 29

Author(s):

Lorena R. Lizarraga-Valderrama ◽

Rinat Nigmatullin ◽

Caroline Taylor ◽

John W. Haycock ◽

Frederik Claeyssens ◽

...

Keyword(s):

Tissue Engineering ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Nerve Tissue ◽

Nerve Tissue Engineering

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In Vitro Evaluation of the Growth and Migration of Schwann Cells on Electrospun Silk Fibroin Nanofibers

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.175-176.220 ◽

2011 ◽

Vol 175-176 ◽

pp. 220-223 ◽

Cited By ~ 2

Author(s):

Ai Jun Hu ◽

Bao Qi Zuo ◽

Feng Zhang ◽

Qing Lan ◽

Huan Xiang Zhang

Keyword(s):

Tissue Engineering ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Schwann Cells ◽

Silk Fibroin ◽

Nerve Tissue ◽

Nerve Tissue Engineering ◽

And Migration ◽

Silk Fibroin Nanofibers

Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.

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Electrofabrication of flexible and mechanically strong tubular chitosan implants for peripheral nerve regeneration

Journal of Materials Chemistry B ◽

10.1039/d1tb00247c ◽

2021 ◽

Author(s):

Hongyu Liu ◽

Yanan Zhao ◽

Jun Tong ◽

Xiaowen Shi ◽

Yun Chen ◽

...

Keyword(s):

Tissue Engineering ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Nerve Tissue ◽

Nerve Tissue Engineering ◽

New Type

The development of peripheral nerve tissue engineering requires safe and reliable methodology to construct biodegradable conduits. Herein, a new type of chitosan-based nerve-guide hydrogel conduit (CNHC) with enhanced mechanical flexibility...

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Improved preparation of acellular nerve scaffold and application of PKH26 fluorescent labeling combined with in vivo fluorescent imaging system in nerve tissue engineering

Neuroscience Letters ◽

10.1016/j.neulet.2013.10.021 ◽

2013 ◽

Vol 556 ◽

pp. 52-57 ◽

Cited By ~ 9

Author(s):

Bin Zhao ◽

Xiaolei Sun ◽

Xiulan Li ◽

Qiang Yang ◽

Yanjun Li ◽

...

Keyword(s):

Tissue Engineering ◽

Imaging System ◽

Fluorescent Labeling ◽

Fluorescent Imaging ◽

Nerve Tissue ◽

Nerve Tissue Engineering

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Genetically modified canine Schwann cells—In vitro and in vivo evaluation of their suitability for peripheral nerve tissue engineering

Journal of Neuroscience Methods ◽

10.1016/j.jneumeth.2009.11.023 ◽

2010 ◽

Vol 186 (2) ◽

pp. 202-208 ◽

Cited By ~ 21

Author(s):

Ruth Schmitte ◽

Andrea Tipold ◽

Veronika M. Stein ◽

Henning Schenk ◽

Cornelia Flieshardt ◽

...

Keyword(s):

Tissue Engineering ◽

Peripheral Nerve ◽

Schwann Cells ◽

Genetically Modified ◽

Nerve Tissue ◽

In Vivo Evaluation ◽

Nerve Tissue Engineering

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Tailoring Nano-Porous Surface of Aligned Electrospun Poly (L-Lactic Acid) Fibers for Nerve Tissue Engineering

International Journal of Molecular Sciences ◽

10.3390/ijms22073536 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3536

Author(s):

Hongyun Xuan ◽

Biyun Li ◽

Feng Xiong ◽

Shuyuan Wu ◽

Zhuojun Zhang ◽

...

Keyword(s):

Tissue Engineering ◽

Lactic Acid ◽

Bacterial Adhesion ◽

Bacterial Colonization ◽

Cellular Responses ◽

Surface Structures ◽

Porous Surface ◽

Nerve Tissue ◽

Proliferation And Differentiation ◽

Nerve Tissue Engineering

Despite the existence of many attempts at nerve tissue engineering, there is no ideal strategy to date for effectively treating defective peripheral nerve tissue. In the present study, well-aligned poly (L-lactic acid) (PLLA) nanofibers with varied nano-porous surface structures were designed within different ambient humidity levels using the stable jet electrospinning (SJES) technique. Nanofibers have the capacity to inhibit bacterial adhesion, especially with respect to Staphylococcus aureus (S. aureus). It was noteworthy to find that the large nano-porous fibers were less detrimentally affected by S. aureus than smaller fibers. Large nano-pores furthermore proved more conducive to the proliferation and differentiation of neural stem cells (NSCs), while small nano-pores were more beneficial to NSC migration. Thus, this study concluded that well-aligned fibers with varied nano-porous surface structures could reduce bacterial colonization and enhance cellular responses, which could be used as promising material in tissue engineering, especially for neuro-regeneration.

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