Preparation and characterization of conductive poly-dl-lactic-acid/tetra-aniline conduit for peripheral nerve regeneration

2018 ◽  
Vol 34 (2) ◽  
pp. 190-208 ◽  
Author(s):  
Xing-Lei Guo ◽  
Hai-Xing Xu ◽  
Qun-Di He ◽  
Yun-Xuan Yu ◽  
Xiao-Fei Ming ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Contact Angles ◽  
Nerve Tissue ◽  
Conductive Composite ◽  
Nerve Tissue Engineering

Defected peripheral nerve regeneration is still a challenge in clinical treatment. Conductive polymers show great potential in nerve tissue engineering because of their electrical property based on bioelectricity in vivo. In this study, conductive composite nerve conduit was synthesized with tetra-aniline and poly-dl-lactic acid. Their properties and the differentiation of rat pheochromocytoma 12 (PC12) cells in vitro stimulated with 200 mV for 1 h were investigated. Different amounts of tetra-aniline (0%, 5%, 10%, and 15%) were used to synthesize the conduits with different conductivities (0, 0.00625, 0.01, and 0.025 s/m, respectively), tensile strengths (2.45, 3.40, 4.45, and 5.50 MPa, respectively), and contact angles (80°, 78.5°, 62.5°, and 61.5°, respectively). The percentage of neurite-bearing cells and the median neurite length increased with an obvious raise of the content of tetra-aniline. In addition, the conduit with subcutaneous implantable experiments in vivo showed less inflammatory response. These promising results illustrated that this poly-dl-lactic acid/tetra-aniline conductive composite conduit had potential for nerve tissue engineering.

scholarly journals Nerve tissue engineering using blends of poly(3-hydroxyalkanoates) for peripheral nerve regeneration

2015 ◽  
Vol 15 (6) ◽  
pp. 612-621 ◽  
Author(s):  
Lorena R. Lizarraga-Valderrama ◽  
Rinat Nigmatullin ◽  
Caroline Taylor ◽  
John W. Haycock ◽  
Frederik Claeyssens ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering

Electrofabrication of flexible and mechanically strong tubular chitosan implants for peripheral nerve regeneration

2021 ◽  
Author(s):  
Hongyu Liu ◽  
Yanan Zhao ◽  
Jun Tong ◽  
Xiaowen Shi ◽  
Yun Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
New Type

The development of peripheral nerve tissue engineering requires safe and reliable methodology to construct biodegradable conduits. Herein, a new type of chitosan-based nerve-guide hydrogel conduit (CNHC) with enhanced mechanical flexibility...

In Vitro Evaluation of the Growth and Migration of Schwann Cells on Electrospun Silk Fibroin Nanofibers

2011 ◽  
Vol 175-176 ◽  
pp. 220-223 ◽  
Author(s):  
Ai Jun Hu ◽  
Bao Qi Zuo ◽  
Feng Zhang ◽  
Qing Lan ◽  
Huan Xiang Zhang
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Silk Fibroin ◽  
Nerve Tissue ◽  
Nerve Tissue Engineering ◽  
And Migration ◽  
Silk Fibroin Nanofibers

Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.

In vivo evaluation of poly(l-lactic acid) porous conduits for peripheral nerve regeneration

Biomaterials ◽  
1999 ◽  
Vol 20 (12) ◽  
pp. 1109-1115 ◽  
Author(s):  
G.R.D. Evans ◽  
K. Brandt ◽  
M.S. Widmer ◽  
L. Lu ◽  
R.K. Meszlenyi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
In Vivo Evaluation

scholarly journals Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hui Liu ◽  
Peizhen Lv ◽  
Yongjia Zhu ◽  
Huayu Wu ◽  
Kun Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Nerve Injury ◽  
Peripheral Nerve Regeneration ◽  
Immunohistochemical Analysis ◽  
Neuroprotective Effects

Abstract Salidriside (SDS), a phenylpropanoid glycoside derived from Rhodiola rosea L, has been shown to be neuroprotective in many studies, which may be promising in nerve recovery. In this study, the neuroprotective effects of SDS on engineered nerve constructed by Schwann cells (SCs) and Poly (lactic-co-glycolic acid) (PLGA) were studied in vitro. We further investigated the effect of combinational therapy of SDS and PLGA/SCs based tissue engineering on peripheral nerve regeneration based on the rat model of nerve injury by sciatic transection. The results showed that SDS dramatically enhanced the proliferation and function of SCs. The underlying mechanism may be that SDS affects SCs growth through the modulation of neurotrophic factors (BDNF, GDNF and CNTF). 12 weeks after implantation with a 12 mm gap of sciatic nerve injury, SDS-PLGA/SCs achieved satisfying outcomes of nerve regeneration, as evidenced by morphological and functional improvements upon therapy by SDS, PLGA/SCs or direct suture group assessed by sciatic function index, nerve conduction assay, HE staining and immunohistochemical analysis. Our results demonstrated the significant role of introducing SDS into neural tissue engineering to promote nerve regeneration.

scholarly journals Tissue Engineering Strategies for Peripheral Nerve Regeneration

2021 ◽  
Vol 12 ◽  
Author(s):  
Yin Li ◽  
Zhenjiang Ma ◽  
Ya Ren ◽  
Dezhi Lu ◽  
Tao Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Injury ◽  
Peripheral Nerve Regeneration ◽  
Neural Tissue ◽  
Nerve Tissue ◽  
Neural Tissue Engineering ◽  
Clinical Problems

A peripheral nerve injury (PNI) has severe and profound effects on the life of a patient. The therapeutic approach remains one of the most challenging clinical problems. In recent years, many constructive nerve regeneration schemes are proposed at home and abroad. Nerve tissue engineering plays an important role. It develops an ideal nerve substitute called artificial nerve. Given the complexity of nerve regeneration, this review summarizes the pathophysiology and tissue-engineered repairing strategies of the PNI. Moreover, we discussed the scaffolds and seed cells for neural tissue engineering. Furthermore, we have emphasized the role of 3D printing in tissue engineering.

scholarly journals Author Correction: Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hui Liu ◽  
Peizhen Lv ◽  
Yongjia Zhu ◽  
Huayu Wu ◽  
Kun Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Schwann Cells ◽  
Peripheral Nerve Regeneration ◽  
Engineering Strategy

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