Allium test I: A 2–3 day plant test for toxicity assessment by measuring the mean root growth of onions (allium cepa L.)

1993 ◽  
Vol 8 (4) ◽  
pp. 461-470 ◽  
Author(s):  
Geirid Fiskesjó
Keyword(s):  
Root Growth ◽  
Allium Cepa ◽  
Toxicity Assessment ◽  
Allium Test ◽  
Allium Cepa L ◽  
Plant Test ◽  
The Mean

scholarly journals Phyto-toxic Influence of an Atrazine-based Selective Herbicide – Arda-force® on Onions (Allium cepa L)

2020 ◽  
pp. 40-46
Author(s):  
Doris Fovwe Ogeleka ◽  
Esther Obasi
Keyword(s):  
Root Growth ◽  
Growth Inhibition ◽  
Allium Cepa ◽  
Environmental Protection Agency ◽  
Toxic Effects ◽  
Root Growth Inhibition ◽  
Allium Cepa L ◽  
Test Concentration ◽  
Maximum Root ◽  
Selective Herbicide

Introduction: The constant impact on the environment occasioned by pollution, indiscriminate application of agricultural chemicals, security challenges and crisis in the Niger Delta ecological area of Nigeria has caused severe damage to plants, soil organisms and humans. Aim and Methodology: In this research, onions (Allium cepa L) was exposed to varying concentrations of an atrazine-based selective herbicide Arda-force® to estimate the phyto-toxic effects on the plant species using the Organization for Economic Co-operation and Development, (OECD) protocol #208. Results: The mean effective concentration (EC50) using root growth inhibition produced indications of phyto-toxicity to the exposed species at a concentration of 0.55 ± 0.06 mg/L. Similarly, the maximum root growth inhibition efficiency relative to the control was 65% as recorded in the highest test concentration of 1.25 mg/L. Discussion: The study indicated that constant application / indiscriminate use of the herbicide Arda-force® could cause deleterious influence on these plant and vegetable species, daily consumed by humans as a rich source of anti-oxidants. Conclusion: This study concluded that atrazine-based herbicide Arda-force® used in this assessment resulted in phyto-toxic effects to Allium cepa L. At the exposed concentrations of the herbicide to non-target specie – Allium cepa L. that are integral parts of the ecosystems, the ‘‘harmless’’ status of atrazine acclaimed by the United State Environmental Protection Agency (USEPA) is still very much in doubt.

Relationship between apoplastic ascorbate regeneration and the stimulation of root growth in Allium cepa L.

Plant Science ◽  
1994 ◽  
Vol 100 (1) ◽  
pp. 23-29 ◽  
Author(s):  
JoséA. González-Reyes ◽  
Francisco J. Alcaín ◽  
JoséA. Caler ◽  
Antonio Serrano ◽  
Francisco Córdoba ◽  
...  
Keyword(s):  
Root Growth ◽  
Allium Cepa ◽  
Allium Cepa L ◽  
Ascorbate Regeneration ◽  
Stimulation Of

Inhibition Effect on the Allium cepa L. Root Growth When Using Hexavalent Chromium-Doped River Waters

2009 ◽  
Vol 82 (6) ◽  
pp. 767-771 ◽  
Author(s):  
F. R. Espinoza-Quiñones ◽  
N. Szymanski ◽  
S. M. Palácio ◽  
A. N. Módenes ◽  
M. A. Rizzutto ◽  
...  
Keyword(s):  
Root Growth ◽  
Hexavalent Chromium ◽  
Allium Cepa ◽  
River Waters ◽  
Inhibition Effect ◽  
Allium Cepa L

scholarly journals Relations between boiling water test, standard germination test and field emergence of leek (Allium porrum L.) and onion (Allium cepa L.) seeds

2012 ◽  
Vol 62 (2) ◽  
pp. 173-178
Author(s):  
Ismail Guvenc ◽  
Haluk C. Kaymak ◽  
Sibel Duman
Keyword(s):  
Allium Cepa ◽  
Seed Viability ◽  
Boiling Water ◽  
Allium Porrum ◽  
Germination Test ◽  
Reliable Technique ◽  
Allium Cepa L ◽  
Water Test ◽  
The Mean ◽  
Field Emergence

The aim of this study was to determine relations occurring between boiling water test, standard germination test and field emergence of leek (<i>Allium porrum</i> L.) and onion (<i>Allium cepa</i> L.) seeds. In this study, seeds of six lots ('Kalem', 'Ala', '&#205;neg&#246;l-A, B, C and D') from three cultivars of leek and seven onion cultivars ('Early Texas Grano' (ETG), 'Panku', 'Storm', 'Banko', 'Aki', 'Kisag&#252;n' and 'Banka') seeds were used as plant material and their viability was evaluated in boiling water test (BWT), standard germination test (SGT) and field emergence (FE). The percentage of field emergence was evaluated at three sowing times: 20 May (FE-I), 10 June (FE-II) and 20 July (FE-III). The mean germination of leek seeds varied from 77.5% to 100.0% and from 36.0% to 61.0% in SGT and BWT, respectively. While the range of results obtained in the boiling water test was from 38.5% to 60.0%, the range of results of the standard germination test was from 81.0% to 100.0% in onion seeds. The range of field emergence was between 18.5% ('Kisag&#252;n', FE-III) and 72.0% (İnegöl-C', FE-II). Besides, the boiling water test was correlated highly significantly with SGT (r = 0.670<sup>**</sup>), FE-I (r = 0.923<sup>**</sup>), FE-II (r = 0.906<sup>**</sup>) and FE-III (r = 0.939<sup>**</sup>) in leek seeds. Similarly, BWT showed positive correlation with SGT (r = 0.568<sup>**</sup>), FE-I (r = 0.844<sup>**</sup>), FE-II (r = 0.933<sup>**</sup>) and FE-III (r = 0.858<sup>**</sup>) in onion seeds. In conclusion, the boiling water test is a new and reliable technique to test seed viability and it has a great potential to test rapidly germination and field emergence of leek and onion seeds at different sowing times.

Estimación de la genotoxicidad del río Sinú mediante un bioensayo con Allium cepa l. en Montería, Córdoba-Colombia

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Teodora Cavadía ◽  
María Roche M ◽  
Rosalyn Romero
Keyword(s):  
Allium Cepa ◽  
Allium Test ◽  
Allium Cepa L ◽  
Del Rio

Las alteraciones son producidas por desechos tóxicos en ambientes contaminados, siendo el ecosistema acuático afectado por vertimientos de estos en su lecho. La implementación de Allium cepa L. como organismo de ensayo es útil para estudios genotóxicos. Esta investigación tuvo como finalidad, estimar la genotoxicidad en muestras de agua del rio Sinú de la ciudad de Montería. Las muestras fueron colectadas en la Vereda Jaraquiel, Avenida Primera y Los Garzones. Luego se realizó el ensayo Allium test, el cual consistió en seleccionar 16 bulbos de cebolla que fueron introducidos en las muestras problema y control. Después de 72 h se cortaron las raíces tiernas y se sumergieron en Aceto-Orceina por 48 h. Posteriormente se llevaron al microscopio, efectuándose un conteo celular y se observaron las anomalías cromosómicas. Se hicieron pruebas de normalidad y análisis de varianza ANOVA. Los índices mitóticos de las fases mostraron diferencias significativas (P=0.0440) y presencia de alteraciones en las muestras problema, presentando Los Garzones el mayor número de alteraciones en la división celular en la etapa anafase con puente, presencia de micronúcleos y otras. Se evidenció genotoxicidad en los puntos estudiados por la influencia de contaminación en el rio Sinú.

scholarly journals Efficiency of the aquatic macrophyte salvinia auriculata in purification of urban effluents, validated by allium test (Allium Cepa L.)

2015 ◽  
Vol 8 (2) ◽  
pp. 29
Author(s):  
Priscila S. Gonçalves ◽  
Camila S. P. Oliveira ◽  
Matheus Prem Mendes ◽  
Marcelo Netto Duarte ◽  
William Costa Rodrigues
Keyword(s):  
Allium Cepa ◽  
Aquatic Macrophytes ◽  
Aquatic Macrophyte ◽  
Sample Treatment ◽  
Allium Test ◽  
Allium Cepa L ◽  
Kolmogorov Smirnov ◽  
Student’S T ◽  
Student’S T Test ◽  
Smirnov Test

This study aimed to evaluate the efficiency of the aquatic macrophytes Salvinia auriculata Aublet in purification of polluted effluents, and evaluate macroscopically the efficiency of macrophytes using the Allium cepa Linnaeus (onion test). Three collections were performed in September 2011, the first analysis was performed with water collected directly from River Santa Catarina, the second was performed seven days after the contact of the effluent with the macrophytes, and the third, fifteen days after the first analyses. The data were analyzed using Student’s t-test (p<0.05). To verify the normality of the data was used the Kolmogorov-Smirnov test (p<0.05), for each sample (treatment). According to the results of the Allium test, the aquatic macrophyte S. auriculata was efficient in the removing of pollutants agents after fifteen days in contact with the effluent.

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