Root Endophytic Fungal Community and Carbon and Nitrogen Stable Isotope Patterns Differ among Bletilla Species (Orchidaceae)

Orchids of the genus Bletilla are well-known ornamental plants and sources of traditional medicine in Asia that rely on the symbiotic relationship with root endophytic fungi throughout their whole life cycle. However, little is known about their fungal partners, infection pattern, and pathways of carbon gain. We investigated carbon and nitrogen stable isotope patterns in different organs of three Bletilla species, identified the root endophytic fungal community composition, and determined mycorrhizal colonization rates. The three Bletilla species were comprised by a polyphyletic group which belongs to different trophic modes, such as saprotroph, pathotroph, and symbiotroph; however, the dominant species and their abundances varied among Bletilla spp. Mycorrhizal infection rates also varied among Bletilla species, with B. striata (65% ± 25%) being significantly higher than those of B. formosana (35% ± 16%) and B. ochracea (22% ± 13%). Compared with surrounding autotrophic plants, all Bletilla spp. were significantly enriched in 13C with B. striata to a significantly higher level than other two Bletilla species. Among different organs, stems had higher δ13C values, while leaves and flowers had higher δ15N and total N content values across all three species. Our results indicate that the symbiotic relationship of Bletilla and its root endophytic fungi is not strictly specific. Although mycorrhizal infection rates were highly variable, the three Bletilla species had the same infection pattern with hyphae penetrating the cortex cell by the pathway cell. Different Bletilla species have different strategies for C allocation among plant organs. These findings provide new insights into the ecological adaptation of orchids and will contribute to Bletilla germplasm conservation and sustainable utilization.

Farklı Mikoriza Türleri, Kompost ve Fosfat Kayacı Uygulamalarının Sorgum Bitkisinin Gelişimine Etkileri

The aim of this study was to determine the effects of different compost and rock phosphate doses applications with different mycorrhizal species inoculation on sorghum plant growth and nutrient uptake. Dosages 0, 20, 40 gr compost kg-1 soil as were used as compost, and 0, 2, 4 gr rock phosphate/soil as rock phosphate sources. Funnelformis mosseae, F. caledonius, Claroideoglomus etunicatum, Rhizophagus clarus, indigenous and cocktail (mixture) mycorrhizae were used. Shoot and root dry matter, P and Zn concentration and root percentages of mycorrhizal infection were determined. According to the research findings, mycorrhiza inoculation significantly increased the shoot and root dry matter, P, Zn and root infection of sorghum plant more than rock phosphate and compost applications. F. caledonius was determined as the most effective species. While 20 and 40 g compost kg-1 soil doses applications were most effective, it was concluded that the shortage of cultivation time resulted that the rock phosphate may not be sufficiently dissolved for nutrient supply to plant growth.

Effects of Nitrogen Fertilization on Mycorrhizal Infection, Nodulation and Growth of Phaseolus vulgaris L.

SummaryThe common bean (Phaseolus vulgaris L.) has been the subject of a number of studies and has been described by many authors as a legume with low nitrogen fixing potential compared to other legume species. The first objective of this study is to assess the development and growth of P. vulgaris L. var. Djedida and its yield at different developmental stages (vegetative, flowering and fruiting), in the presence and absence of nitrogen fertilizer (Urea 46) in an arid climate and in a soil containing high level of CaCO3 and assimilable phosphorus. The second objective is to carry out a survey on nodulation, number of spores and mycorrhizal infection under these conditions. The results showed that the nitrogen fertilizer amendment is mainly significant (p< 0.0001) for various morphological parameters. Indeed, great improvement was observed on the majority of the morphological parameters with considerable percentages. However, no significant effect was reported for the roots length. In addition, a negligible number of nodules were obtained in the plot without fertilization and no significant effect on the number of spores was recorded. Furthermore, the effect of fertilization on the arbuscular intensity (a%, A%) and on mycorrhizal colonization (M%, m%) of the roots was found to be significant. The soil in the arid region of Biskra showed significant mycorrhizogenic potential, although the conditions in this region were not very favorable for their development, which could constitute an effective biological resource to improve the tolerance of host plants to biotic and abiotic constraints.

Respons Pertumbuhan Bibit Salak (Salacca edulis L.) terhadap Dosis Spora Endomikoriza pada Media Pembawa Pasir Vulkanik dan Pasir Laut

Response of Salak Seedling (Salacca edulis L.) Growth to Endomycorrhizal Spore Doses on Volcanic Sand and Sea Sand Carrier. Endomycorrhizae has several benefits, such as increase water absorption and nutrients of plant, protect plants from root pathogens and toxic substances, play a role in improving soil structure, and increase nutrient solubility. This study aimed to determine the growth response of salak seedlings to doses of endomycorrhizal spores in media carrying volcanic sand and sea sand. This study used a factorial randomized block design (RBD) with two treatments i.e. the number of spores and the type of carriers media. The results showed no significant interaction between the number of mycorrhizal spores doses with the carrier media on all observed variables. Spores doses significantly affected stem diameter and root mycorrhizal infection variables. The highest stem diameter was achieved at the dose of 75 spores that was 1.60 cm which was significantly different from control (1.28 cm), whereas the highest root infection was obtained at the dose of 225 spores (92.50%) which significantly higher than control (36.25%). At the observation of 4 MAP (month after planting) the highest root infection was obtained at the dose of 150 spores (82.50%) compared to control (10.00%). Volcanic sand carrier media produced the highest plant dry weight (3.59 g) which was significantly different to the sea sand (2.43 g).

Potential of Native Arbuscular Mycorrhizal Fungi, Rhizobia, and/or Green Compost as Alfalfa (Medicago sativa) Enhancers under Salinity

Salinity is one of the devastating abiotic stresses that cause reductions in agricultural production. The increased salinization affects alfalfa growth, metabolism, and rhizobium capacity for symbiotic N2 fixation negatively. This study was undertaken to investigate the efficiency of green compost (C; made from green waste), arbuscular mycorrhizal fungi (M; field-sourced native consortium), and/or rhizobium (R; a salt-tolerant rhizobium strain) individually or in combination as an effective strategy to improve alfalfa productivity under non-saline and high-saline (120 mM NaCl) conditions. In addition, we aimed to understand the agro-physiological and metabolic basis as well as glomalin content in the soil of biofertilizers-induced salt tolerance in alfalfa. Here, we show that mycorrhizal infection was enhanced after MR inoculation, while C application decreased it significantly. Salinity reduced growth, physiological functioning, and protein concentration, but the antioxidant system has been activated. Application of the selected biofertilizers, especially C alone or combined with M and/or R improved alfalfa tolerance. The tri-combination CMR mitigated the negative effects of high salinity by stimulating plant growth, roots and nodules dry matters, mineral uptake (P, N, and K), antioxidant system, synthesis of compatible solutes, and soil glomalin content, sustaining photosynthesis-related performance and decreasing Na+ and Cl− accumulation, lipid peroxidation, H2O2 content, and electrolyte leakage.

Uji Efektifvitas Cendawan Mikoriza Lokal Gerogak Lahan Kering dan Komersial Pada Tanaman Jagung (Zea mays L.)

Dryland in Indonesia has been dominated by acid soils, so that availability of phosphate (P) becomes the main obstacle in improving outcomes and only 10-30% of fertilizer P can be utilized by plants. One of the solution to meet the needs of phosphate is by utilization of mycorrhizal biofertilizer. These experiments were divided into several phases: 1) Looking for phases of mycorrhizal, 2) the laboratory analysis, 3) Testing phase on plants that were carried out by using polybags with sterile soil. The experiment was designed with a complete randomized block design (RAKL) by using a factor that consists of 6 treatments that are: 1) Without mycorrhizal inoculum (M0), 2) Mycorrhizal inoculum 5 g + 50 Spores (M5), 3) Mycorrhizal inoculum 10 g + 50 spores (M10), 4) Mycorrhizal inoculum 15 g + 50 spores (M15), 5) Mycorrhizal inoculum 20 g + 50 spores (M20), 6) Commercial mycorrhizal 10 g (Mk). All treatments were repeated four times so that the total 24 pot experiment. The experiment results show that mycorrhizal treatment given on the corn crop has very significant (P <0.01) to the number of leaf age of 35, 49, 63 days after, leaf wide have age 49 days after plant, fresh weight, oven-dry weight, absorb P, number of spores, mycorrhizal infection and significant effect (P <0.05) to plant height age of 35, 49, 63 day after plant, 63 days after plant, leaf wide, and P-available. Based on the results of this study will be suggested, in order to enhance the growth of corn plants can be used locally mikroiza a dose of 10 g + 50 spores.

Kombinasi Pupuk Kandang Sapi, Asam Humat Dan Mikoriza Terhadap Infeksi Akar Bermikoriza Tanaman Cabai Dan Ketersediaan Unsur Hara Tanah Udipsamments

This research is an experimental study that aims to examine the combination of cattle manure, humic acid, and mycorrhizae to increase mycorrhizal infections in the roots of chili plants in the Udipsamments soil in Puger District, Jember Regency, which was carried out in May-November 2018. The research design used was a factorial complete randomized block design with 3 factors that were repeated 3 times. The first factor is cattle manure (0 tons / ha, 10 tons / ha, 20 tons / ha), the second factor is humic acid (0 ppm / ha, 200 ppm / ha), and the third factor is mycorrhiza (0 g / plant , 30 g / plant). The results showed that the combination of cattle manure, humic acid, and mycorrhiza inoculants to chili plant roots was able to increase the root mycorrhizal infection of chili plants and increase the nutrients available in the soil by 191.84% for nitrogen and 392.93% for phosphorus.