Comparison and evolutionary analysis of Brassica nucleotide binding site leucine rich repeat (NLR) genes and importance for disease resistance breeding

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

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Faculty Opinions recommendation of A gain-of-function mutation in an Arabidopsis Toll Interleukin1 receptor-nucleotide binding site-leucine-rich repeat type R gene triggers defense responses and results in enhanced disease resistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1011118.178464 ◽

2003 ◽

Author(s):

John W Mansfield

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Nucleotide Binding ◽

Defense Responses ◽

Nucleotide Binding Site ◽

R Gene ◽

Leucine Rich Repeat ◽

Repeat Type ◽

Gain Of Function

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A Gain-of-Function Mutation in an Arabidopsis Toll Interleukin1 Receptor–Nucleotide Binding Site–Leucine-Rich Repeat Type R Gene Triggers Defense Responses and Results in Enhanced Disease Resistance

The Plant Cell ◽

10.1105/tpc.005348 ◽

2002 ◽

Vol 14 (12) ◽

pp. 3149-3162 ◽

Cited By ~ 185

Author(s):

Yumiko Shirano ◽

Pradeep Kachroo ◽

Jyoti Shah ◽

Daniel F. Klessig

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Nucleotide Binding ◽

Defense Responses ◽

Nucleotide Binding Site ◽

R Gene ◽

Leucine Rich Repeat ◽

Repeat Type ◽

Gain Of Function

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Deletion of a Disease Resistance Nucleotide-Binding-Site Leucine-Rich- Repeat-like Sequence Is Associated With the Loss of the Phytophthora Resistance Gene Rps4 in Soybean

Genetics ◽

10.1534/genetics.104.032037 ◽

2004 ◽

Vol 168 (4) ◽

pp. 2157-2167 ◽

Cited By ~ 70

Author(s):

Devinder Sandhu ◽

Hongyu Gao ◽

Silvia Cianzio ◽

Madan K. Bhattacharyya

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Resistance Gene ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Phytophthora Resistance

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Genome-wide identification and characterization of nucleotide binding site leucine-rich repeat genes in linseed reveal distinct patterns of gene structure

Genome ◽

10.1139/gen-2012-0135 ◽

2013 ◽

Vol 56 (2) ◽

pp. 91-99 ◽

Cited By ~ 8

Author(s):

Sandip M. Kale ◽

Varsha C. Pardeshi ◽

Vitthal T. Barvkar ◽

Vidya S. Gupta ◽

Narendra Y. Kadoo

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Regulatory Element ◽

Nucleotide Binding ◽

Defense Responses ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Quantitative Expression ◽

Domain Arrangement ◽

Gene Structures

Plants employ different disease-resistance genes to detect pathogens and to induce defense responses. The largest class of these genes encodes proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) domains. To identify the putative NBS–LRR encoding genes from linseed, we analyzed the recently published linseed genome sequence and identified 147 NBS–LRR genes. The NBS domain was used for phylogeny construction and these genes were classified into two well-known families, non-TIR (CNL) and TIR related (TNL), and formed eight clades in the neighbor-joining bootstrap tree. Eight different gene structures were observed among these genes. An unusual domain arrangement was observed in the TNL family members, predominantly in the TNL-5 clade members belonging to class D. About 12% of the genes observed were linseed specific. The study indicated that the linseed genes probably have an ancient origin with few progenitor genes. Quantitative expression analysis of five genes showed inducible expression. The in silico expression evidence was obtained for a few of these genes, and the expression was not correlated with the presence of any particular regulatory element or with unusual domain arrangement in those genes. This study will help in understanding the evolution of these genes, the development of disease resistant varieties, and the mechanism of disease resistance in linseed.

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