Computational Identification of MicroRNA-targeted Nucleotide-Binding Site-Leucine-Rich Repeat Genes in Plants

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

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Molecular characterization and functional analysis of CzR1, a coiled-coil-nucleotide-binding-site-leucine-rich repeat R-gene from Curcuma zedoaria Loeb. that confers resistance to Pythium aphanidermatum

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2013.05.003 ◽

2013 ◽

Vol 83 ◽

pp. 59-68 ◽

Cited By ~ 13

Author(s):

Basudeba Kar ◽

Satyabrata Nanda ◽

Pradeep Kumar Nayak ◽

Sanghamitra Nayak ◽

Raj Kumar Joshi

Keyword(s):

Functional Analysis ◽

Binding Site ◽

Molecular Characterization ◽

Coiled Coil ◽

Nucleotide Binding ◽

Pythium Aphanidermatum ◽

Nucleotide Binding Site ◽

R Gene ◽

Leucine Rich Repeat ◽

Curcuma Zedoaria

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Importance of OsRac1 and RAI1 in signalling of nucleotide‐binding site leucine‐rich repeat protein‐mediated resistance to rice blast disease

New Phytologist ◽

10.1111/nph.15816 ◽

2019 ◽

Vol 223 (2) ◽

pp. 828-838 ◽

Cited By ~ 5

Author(s):

Zhuangzhi Zhou ◽

Zhiqian Pang ◽

Shengli Zhao ◽

Lingli Zhang ◽

Qiming Lv ◽

...

Keyword(s):

Binding Site ◽

Rice Blast ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Rice Blast Disease ◽

Blast Disease ◽

Leucine Rich Repeat ◽

Repeat Protein ◽

Leucine Rich Repeat Protein

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Long-Term Evolution of Nucleotide-Binding Site-Leucine-Rich Repeat Genes: Understanding Gained from and beyond the Legume Family

PLANT PHYSIOLOGY ◽

10.1104/pp.114.243626 ◽

2014 ◽

Vol 166 (1) ◽

pp. 217-234 ◽

Cited By ~ 54

Author(s):

Zhu-Qing Shao ◽

Yan-Mei Zhang ◽

Yue-Yu Hang ◽

Jia-Yu Xue ◽

Guang-Can Zhou ◽

...

Keyword(s):

Binding Site ◽

Nucleotide Binding ◽

Long Term Evolution ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Term Evolution

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Dm3 Is One Member of a Large Constitutively Expressed Family of Nucleotide Binding Site—Leucine-Rich Repeat Encoding Genes

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2002.15.3.251 ◽

2002 ◽

Vol 15 (3) ◽

pp. 251-261 ◽

Cited By ~ 62

Author(s):

Katherine A. Shen ◽

Doris B. Chin ◽

Rosa Arroyo-Garcia ◽

Oswaldo E. Ochoa ◽

Dean O. Lavelle ◽

...

Keyword(s):

Binding Site ◽

Downy Mildew ◽

Point Mutations ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Intron Splice ◽

Major Cluster ◽

Wide Range ◽

Encoding Genes

The major cluster of resistance genes in lettuce cv. Diana contains approximately 32 nucleotide binding site—leucine-rich repeat encoding genes. Previous molecular dissection of this complex region had identified a large gene, RGC2B, as a candidate for encoding the downy mildew resistance gene, Dm3. This article describes genetic and transgenic complementation data that demonstrated RGC2B is necessary and sufficient to confer resistance with Dm3 specificity. Ethylmethanesulphonate was used to induce mutations to downy mildew susceptibility in cv. Diana (Dm1, Dm3, Dm7, and Dm8). Nineteen families were identified with a complete loss of resistance in one of the four resistance specificities. Sequencing revealed a variety of point mutations in RGC2B in the six dm3 mutants. Losses of resistance were due to single changes in amino acid sequence or a change in an intron splice site. These mutations did not cluster in any particular region of RGC2B. A full-length ge-nomic copy of RGC2B was isolated from a lambdaphage library and introduced into two genotypes of lettuce. Trans-genics expressing RGC2B exhibited resistance to all isolates expressing Avr3 from a wide range of geographical origins. In a wildtype Dm3-expressing genotype, many of the RGC2 family members are expressed at low levels throughout the plant.

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Fine Mapping and Identification of Nucleotide Binding Site/Leucine-Rich Repeat Sequences at the MER Locus in Populus deltoides ‘S9-2’

Phytopathology ◽

10.1094/phyto.2001.91.11.1069 ◽

2001 ◽

Vol 91 (11) ◽

pp. 1069-1073 ◽

Cited By ~ 27

Author(s):

J. Zhang ◽

M. Steenackers ◽

V. Storme ◽

S. Neyrinck ◽

M. Van Montagu ◽

...

Keyword(s):

Binding Site ◽

Genetic Map ◽

Positional Cloning ◽

Populus Deltoides ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Aflp Markers ◽

Interspecific Crosses ◽

Leucine Rich Repeat ◽

Starting Point

Melampsora larici-populina is the most damaging leaf pathogen for poplar in Europe. Previous genetic analyses have revealed both qualitative and quantitative resistance to this fungus. As a starting point for positional cloning of the gene or genes conferring qualitative resistance to M. larici-populina races E1, E2, and E3, a local genetic map of the Melampsora resistance (MER) locus was constructed based on amplified fragment length polymorphism (AFLP) markers. Eleven AFLP markers were identified by bulked segregant analysis. These markers were used to identify 17 recombinants at the MER locus, from a total of 512 progenies derived from three interspecific crosses involving the same resistant female parent, Populus deltoides ‘S9-2’. The local genetic map covered a 3.4-centimorgan interval encompassing the target locus. Sequence analysis of these AFLP markers revealed similarities to the nucleotide binding site/leucine-rich repeat class of disease resistance genes.

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