XI. Yeast sequencing reports. Sequence of a 20·7 kb region of yeast chromosome XI includes theNUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAS for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins

Citrus psorosis virus (CPsV) causes a citrus disease occurring worldwide. Isolate CPV 4 has a genome with three single-stranded RNAs. The complete sequence of RNA 2 (1643 nucleotides) is reported here. Northern blot hybridization with strand-specific probes showed that most of the encapsidated RNA 2 is of negative polarity, although a small amount of the complementary strand may also be present in particles. The RNA 2 complementary strand contained a single open reading frame encoding a protein of 476 amino acids, which includes a motif resembling a nuclear localization signal. The sequence of this putative protein shows no significant similarity to any other in the databases. In the 3′-terminal untranslated region there is a putative polyadenylation signal. No subgenomic RNAs derived from RNA 2 were detected.

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XI. Yeast sequencing reports. Sequence of a 28·6 kb region of yeast chromosome XI includes theFBA1 andTOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins

Yeast ◽

10.1002/yea.320100008 ◽

1994 ◽

Vol 10 (S1994A) ◽

pp. S63-S68 ◽

Cited By ~ 17

Author(s):

Søren W. Rasmussen

Keyword(s):

Storage Proteins ◽

Open Reading Frame ◽

Reading Frame ◽

Yeast Chromosome ◽

Plant Storage

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Cloning and Sequencing of theSphingomonas (Pseudomonas)paucimobilis Gene Essential for the O Demethylation of Vanillate and Syringate

Applied and Environmental Microbiology ◽

10.1128/aem.64.3.836-842.1998 ◽

1998 ◽

Vol 64 (3) ◽

pp. 836-842 ◽

Cited By ~ 45

Author(s):

Seiji Nishikawa ◽

Tomonori Sonoki ◽

Tatsuhide Kasahara ◽

Takahiro Obi ◽

Shoko Kubota ◽

...

Keyword(s):

Open Reading Frame ◽

Cosmid Library ◽

Sequencing Analysis ◽

Cloning And Sequencing ◽

Significant Similarity ◽

Reading Frame ◽

Formyltetrahydrofolate Synthetase ◽

Dna Fragment ◽

Pseudomonas Paucimobilis ◽

Dimeric Model

ABSTRACT Sphingomonas (Pseudomonas)paucimobilis SYK-6 is able to grow on 5,5′-dehydrodivanillic acid (DDVA), syringate, vanillate, and other dimeric model compounds of lignin as a sole carbon source. Nitrosoguanidine mutagenesis of S. paucimobilis SYK-6 was performed, and two mutants with altered DDVA degradation pathways were isolated. The mutant strain NT-1 could not degrade DDVA, but could degrade syringate, vanillate, and 2,2′,3′-trihydroxy-3-methoxy-5,5′-dicarboxybiphenyl (OH-DDVA). Strain DC-49 could slowly assimilate DDVA, but could degrade neither vanillate nor syringate, although it could degrade protocatechuate and 3-O-methylgallate. A complementing DNA fragment of strain DC-49 was isolated from the cosmid library of strain SYK-6. The minimum DNA fragment complementing DC-49 was determined to be the 1.8-kbp insert of pKEX2.0. Sequencing analysis showed an open reading frame of 1,671 bp in this fragment, and a similarity search indicated that the deduced amino acid sequence of this open reading frame had significant similarity (60%) to the formyltetrahydrofolate synthetase ofClostridium thermoaceticum.

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