Optimization of cyclopropane fatty acids production inYarrowia lipolytica

Yeast ◽  
2019 ◽  
Author(s):  
Quentin Czerwiec ◽  
Abdelghani Idrissitaghki ◽  
Nabila Imatoukene ◽  
Maurice Nonus ◽  
Brigitte Thomasset ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cyclopropane Fatty Acids

scholarly journals Enzymatic Synthesis of Cyclopropane Fatty Acids Catalyzed by Bacterial Extracts

1963 ◽  
Vol 238 (4) ◽  
pp. 1242-1248
Author(s):  
H. Zalkin ◽  
John H. Law ◽  
Howard Goldfine
Keyword(s):  
Fatty Acids ◽  
Enzymatic Synthesis ◽  
Cyclopropane Fatty Acids

Total Synthesis of Cardiolipins Containing Chiral Cyclopropane Fatty Acids

2017 ◽  
Vol 82 (15) ◽  
pp. 7832-7838 ◽  
Author(s):  
Shinsuke Inuki ◽  
Ippei Ohta ◽  
Shunichi Ishibashi ◽  
Masayuki Takamatsu ◽  
Koichi Fukase ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Total Synthesis ◽  
Cyclopropane Fatty Acids

scholarly journals Dietary fatty acids promote lipid droplet diversity through seipin enrichment in an ER subdomain

2018 ◽  
Author(s):  
Zhe Cao ◽  
Yan Hao ◽  
Yiu Yiu Lee ◽  
Pengfei Wang ◽  
Xuesong Li ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Lipid Droplet ◽  
Mammalian Cells ◽  
Dietary Fatty Acids ◽  
Fat Storage ◽  
Over Expression ◽  
C Elegans ◽  
Cyclopropane Fatty Acids ◽  
Host Metabolism

AbstractExogenous metabolites from microbial and dietary origins have profound effects on host metabolism. Here, we report that a sub-population of lipid droplets (LDs), which are conserved organelles for fat storage, is defined by metabolites-driven targeting of theC. elegansseipin ortholog, SEIP-1. Loss of SEIP-1 function reduced the size of a subset of LDs while over-expression of SEIP-1 had the opposite effect. Ultrastructural analysis revealed SEIP-1 enrichment in an endoplasmic reticulum (ER) subdomain, which co-purified with LDs. Analyses ofC. elegansand bacterial genetic mutants indicated a requirement of polyunsaturated fatty acids (PUFAs) and microbial cyclopropane fatty acids (CFAs) for SEIP-1 enrichment, as confirmed by dietary supplementation experiments. In mammalian cells, heterologous expression of SEIP-1 promoted lipid droplet expansion from ER subdomains in a conserved manner. Our results suggest that microbial and polyunsaturated fatty acids serve unexpected roles in regulating cellular fat storage by enforcing LD diversity.

Synthesis of biosynthetic inhibitors of the sex pheromone of Spodoptera littoralis. Part II: acetylenic and cyclopropane fatty acids

1992 ◽  
Vol 61 (2) ◽  
pp. 157-167 ◽  
Author(s):  
F. Camps ◽  
S. Hospital ◽  
G. Rosell ◽  
A. Delgado ◽  
A. Guerrero
Keyword(s):  
Fatty Acids ◽  
Sex Pheromone ◽  
Spodoptera Littoralis ◽  
Cyclopropane Fatty Acids

Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria.

1983 ◽  
Vol 18 (6) ◽  
pp. 1370-1377 ◽  
Author(s):  
M A Lambert ◽  
C W Moss
Keyword(s):  
Fatty Acids ◽  
Cyclopropane Fatty Acids ◽  
Acid And Base

A Ring to Rule Them All: The Effect of Cyclopropane Fatty Acids on the Fluidity of Lipid Bilayers

2015 ◽  
Vol 119 (17) ◽  
pp. 5487-5495 ◽  
Author(s):  
David Poger ◽  
Alan E. Mark
Keyword(s):  
Fatty Acids ◽  
Lipid Bilayers ◽  
Cyclopropane Fatty Acids

The path of hydrogen in the formation of cyclopropane fatty acids

1963 ◽  
Vol 70 ◽  
pp. 583-585
Author(s):  
Stephen Pohl ◽  
John H. Law ◽  
Ragnar Ryhage
Keyword(s):  
Fatty Acids ◽  
Cyclopropane Fatty Acids

scholarly journals Determination of Cyclopropane Fatty Acids in Food of Animal Origin by 1H NMR

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Veronica Lolli ◽  
Angela Marseglia ◽  
Gerardo Palla ◽  
Emanuela Zanardi ◽  
Augusta Caligiani
Keyword(s):  
Fatty Acids ◽  
Dairy Products ◽  
Cyclopropane Ring ◽  
Food Sources ◽  
Animal Origin ◽  
Animal Fats ◽  
H Nmr ◽  
Cyclopropane Fatty Acids ◽  
Food Of Animal Origin ◽  
Full Scan

Cyclopropane fatty acids (CPFAs) are unusual fatty acids of microbial origin, recently detected in milk and dairy products. CPFAs have been demonstrated to be interesting molecular markers for authentication of dairy products obtained without ensiled feeds. Moreover, they can also be recognized as a new secondary component of human diet. Information is lacking on the presence of cyclic fatty acids in other food sources. Cyclopropane fatty acids have been detected by GC-MS analysis in cheese and other animal fats in concentration ranging from 200 to 1000 mg/kg fat, but in some cases, the complex fatty acid profile and the possible presence of interfering peaks make the separation not straightforward and the quantification uneasy. Therefore, a new reliable 1H NMR method was developed to detect and measure CPFA content in different foods of animal origin, based on the detection of the characteristic signals of cyclopropane ring. The 1H NMR (600 MHz) method showed detection limits comparable with those of full scan GC-MS, and it allowed the identification and quantitation of the cyclopropane fatty acids in different foods.

Characterization of Pseudomonas aeruginosa fatty acid profiles in biofilms and batch planktonic cultures

2010 ◽  
Vol 56 (12) ◽  
pp. 1028-1039 ◽  
Author(s):  
Jerry Chao ◽  
Gideon M. Wolfaardt ◽  
Michael T. Arts
Keyword(s):  
Fatty Acids ◽  
Pseudomonas Aeruginosa ◽  
Fatty Acid ◽  
Saturated Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Bulk Liquid ◽  
Strain Pao1 ◽  
Fatty Acid Chain ◽  
Cyclopropane Fatty Acids ◽  
Gradual Loss

The fatty acid composition of Pseudomonas aeruginosa PAO1 was compared between biofilm and batch planktonic cultures. Strain PAO1 biofilms were able to maintain a consistent fatty acid profile for up to 6 days, whereas strain PAO1 batch planktonic cultures showed a gradual loss of cis-monounsaturated fatty acids over 4 days. Biofilms exhibited a greater proportion of hydroxy fatty acids but a lower proportion of both cyclopropane fatty acids and saturated fatty acids (SAFAs). SAFAs with ≥16 carbons, in particular, decreased in biofilms when compared with that in batch planktonic cultures. A reduced proportion of SAFAs and a decline in overall fatty acid chain length indicate more fluidic biophysical properties for cell membranes of P. aeruginosa in biofilms. Separating the biofilms into 2 partitions and comparing their fatty acid compositions revealed additional trends that were not observed in the whole biofilm: the shear-nonremovable layer consistently showed greater proportions of hydroxy fatty acid than the bulk liquid + shear-removable portion of the biofilm. The shear-nonremovable portion demonstrated a relatively immediate decline in the proportion of monounsaturated fatty acids between days 2 and 4; which was offset by an increase in the proportion of cyclopropane fatty acids, specifically 19:0cyc(11,12). Simultaneously, the shear-removable portion of the biofilm showed an increase in the proportion of trans-monounsaturated fatty acids and cyclopropane fatty acids.

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