Cloning, Characterization, and Heterologous Expression of cDNAs for Farnesyl Diphosphate Synthase from the Guayule Rubber Plant Reveals That This Prenyltransferase Occurs in Rubber Particles

1996 ◽  
Vol 332 (1) ◽  
pp. 196-204 ◽  
Author(s):  
Zhiqiang Pan ◽  
Lisa Herickhoff ◽  
Ralph A. Backhaus
Keyword(s):  
Heterologous Expression ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Rubber Plant ◽  
Rubber Particles

scholarly journals Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli

2009 ◽  
Vol 75 (22) ◽  
pp. 7291-7293 ◽  
Author(s):  
Gopal Prasad Ghimire ◽  
Hei Chan Lee ◽  
Jae Kyung Sohng
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Streptomyces Peucetius ◽  
E Coli ◽  
Expression Of Genes ◽  
Genes Encoding

ABSTRACT Putative hopanoid genes from Streptomyces peucetius were introduced into Escherichia coli to improve the production of squalene, an industrially important compound. High expression of hopA and hopB (encoding squalene/phytoene synthases) together with hopD (encoding farnesyl diphosphate synthase) yielded 4.1 mg/liter of squalene. This level was elevated to 11.8 mg/liter when there was also increased expression of dxs and idi, E. coli genes encoding 1-deoxy-d-xylulose 5-phosphate synthase and isopentenyl diphosphate isomerase.

Molecular Cloning, Characterisation, and Heterologous Expression of Farnesyl Diphosphate Synthase from Sanghuangporus baumii

2020 ◽  
Vol 62 (2) ◽  
pp. 132-141 ◽  
Author(s):  
Xutong Wang ◽  
Tingting Sun ◽  
Jian Sun ◽  
Shixin Wang ◽  
Yisha Ma ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Molecular Cloning ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase

scholarly journals Inhibitors of Leishmania Major Farnesyl Diphosphate Synthase: Crystallographic and Calorimetric Studies

2011 ◽  
Vol 100 (3) ◽  
pp. 218a-219a
Author(s):  
Srinivas Aripirala ◽  
Sandra Gabelli ◽  
Eric Oldfield ◽  
Dolores Gonzalez Pacanowska ◽  
Mario Amzel
Keyword(s):  
Leishmania Major ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Calorimetric Studies

scholarly journals Farnesyl Diphosphate Synthase Inhibitors from In Silico Screening

2013 ◽  
Vol 81 (6) ◽  
pp. 742-748 ◽  
Author(s):  
Steffen Lindert ◽  
Wei Zhu ◽  
Yi‐Liang Liu ◽  
Ran Pang ◽  
Eric Oldfield ◽  
...  
Keyword(s):  
In Silico ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
In Silico Screening

Production of alarm pheromone starts at embryo stage and is modulated by rearing conditions and farnesyl diphosphate synthase genes in the bird cherry-oat aphid Rhopalosiphum padi

2019 ◽  
Vol 109 (6) ◽  
pp. 821-830 ◽  
Author(s):  
C.-X. Sun ◽  
Z.-X. Li
Keyword(s):  
Alarm Pheromone ◽  
Quantitative Polymerase Chain Reaction ◽  
Rhopalosiphum Padi ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Nymphal Stage ◽  
Rearing Conditions ◽  
Old Adult ◽  
Embryo Stage ◽  
Aphid Alarm Pheromone

AbstractThe major component of aphid alarm pheromone is (E)-β-farnesene (EβF), but the molecular mechanisms of EβF synthesis are poorly understood. Here we established a biological model to study the modulation of EβF synthesis in the bird cherry-oat aphid Rhopalosiphum padi by using quantitative polymerase chain reaction, gas chromatography/mass spectrometry and RNA interference. Our results showed that the rearing conditions significantly affected the weight of adult and modulated EβF synthesis in a transgenerational manner. Specifically, the quantity of EβF per milligram of aphid was significantly reduced in the individually reared adult or 1st-instar nymphs derived from 1-day-old adult reared individually, but EβF in the nymph derived from 2-day-old adult that experienced collective conditions returned to normal. Further study revealed that the production of EβF started in embryo and was extended to early nymphal stage, which was modulated by farnesyl diphosphate synthase genes (RpFPPS1 and RpFPPS2) and rearing conditions. Knockdown of RpFPPS1 and RpFPPS2 confirmed the role played by FPPS in the biosynthesis of aphid alarm pheromone. Our results suggested that the production of EβF starts at the embryo stage and is modulated by FPPS and rearing conditions in R. padi, which sheds lights on the modulatory mechanisms of EβF in the aphid.

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