Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli
2009 ◽
Vol 75
(22)
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pp. 7291-7293
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Keyword(s):
E Coli
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ABSTRACT Putative hopanoid genes from Streptomyces peucetius were introduced into Escherichia coli to improve the production of squalene, an industrially important compound. High expression of hopA and hopB (encoding squalene/phytoene synthases) together with hopD (encoding farnesyl diphosphate synthase) yielded 4.1 mg/liter of squalene. This level was elevated to 11.8 mg/liter when there was also increased expression of dxs and idi, E. coli genes encoding 1-deoxy-d-xylulose 5-phosphate synthase and isopentenyl diphosphate isomerase.
2007 ◽
Vol 103
(6)
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pp. 575-577
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1999 ◽
Vol 181
(15)
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pp. 4499-4504
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2009 ◽
Vol 73
(1)
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pp. 186-188
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2016 ◽
Vol 11
(9)
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pp. 1934578X1601100
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2019 ◽
2002 ◽
Vol 290
(2)
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pp. 869-873
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